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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences International Journal of Animal Science
Pranata, Wahyu Suradi
Fakultas Peternakan, Universitas Persatuan Islam Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Glycerol Utilization as Substrate for Ethanol Production in Escherichia coli Recombinant under an Aerobic Condition Pranata, Wahyu Suradi; Suryadarma, Prayoga; Mangunwidjaja, Djumali
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1142.696 KB) | DOI: 10.11594/jtls.08.01.14

Abstract

The utilization of glycerol as a substrate for ethanol production in E. coli recombinant harboring ethanologenic gens (PDC and ADHB) under aerobic conditions was investigated. This research was conducted by using E. coli BW25113 which compared the growth characteristics on glycerol and glucose as carbon source. E. coli can grow well on both substrates and it consumed glycerol faster than glucose. On glucose, E. coli occurred overflow metabolism that indicated by high acetate accumulation. Meanwhile, on substrate of glycerol the acetate accumulation could be reduced. Using glycerol substrate on E. coli increased piruvate accumulation, comparing with glucose. Therefore, the growth characteristic of glycerol was more effective. In E. coli ∆pta/pHfdh/pTadhB-pdc utilized glycerol substrate was able to accumulate pyruvate as intermediate metabolite for produce 2.18 gL-1 ethanol.
Glycerol Utilization as Substrate for Ethanol Production in Escherichia coli Recombinant under an Aerobic Condition Wahyu Suradi Pranata; Prayoga Suryadarma; Djumali Mangunwidjaja
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.14

Abstract

The utilization of glycerol as a substrate for ethanol production in E. coli recombinant harboring ethanologenic gens (PDC and ADHB) under aerobic conditions was investigated. This research was conducted by using E. coli BW25113 which compared the growth characteristics on glycerol and glucose as carbon source. E. coli can grow well on both substrates and it consumed glycerol faster than glucose. On glucose, E. coli occurred overflow metabolism that indicated by high acetate accumulation. Meanwhile, on substrate of glycerol the acetate accumulation could be reduced. Using glycerol substrate on E. coli increased piruvate accumulation, comparing with glucose. Therefore, the growth characteristic of glycerol was more effective. In E. coli ∆pta/pHfdh/pTadhB-pdc utilized glycerol substrate was able to accumulate pyruvate as intermediate metabolite for produce 2.18 gL-1 ethanol.
Pemanfaatan Gliserol Lemak Hewani untuk Produksi Bioetanol pada Escherichia coli Rekombinan Wahyu Suradi Pranata
International Journal of Animal Science Vol. 5 No. 03 (2023): International Journal of Animal Science
Publisher : Litbang PEMAS Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/asj.v5i03.107

Abstract

Mengoptimalkan pemanfaatan gliserol dari transesterifikasi lemak hewani sebagai substrat untuk produksi etanol dalam etanologenik rekombinan E. coli (PDC dan ADH) dalam kondisi aerobik telah diselidiki. Penelitian ini dilakukan dengan menggunakan E. coli BW25113 yang membandingkan karakteristik pertumbuhan pada substrat gliserol dan glukosa. E. coli dapat tumbuh dengan baik pada kedua substrat tersebut dan gliserolnya lebih cepat dikonsumsi daripada glukosa. Pada glukosa, E. coli terjadi overflow metabolisme yang menandakan tingginya jumlah asetat, sedangkan pada gliserol akumulasi asetat dapat dikurangi. Sehingga karakteristik pertumbuhan gliserol lebih efektif. Pada E. coli ∆pta/pHfdh/pTadhB-pdc produksi etanol dengan substrat gliserol adalah 2,18 gL-1 Angka tersebut dua kali lebih tinggi dibandingkan dengan substrat glukosa dan gliserol sebagai substrat memiliki kondisi optimal dalam menyediakan NADH untuk menghasilkan etanol.
Co-Authors Djumali Mangunwidjaja Djumali Mangunwidjaja Prayoga Suryadarma Sapta Raharja Prayoga Suryadarma dan T. Ikhsan Azmi Ani Suryani