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Immunohistochemistry Evaluation of TGF-β1, SOX-9, Type II Collagen and Aggrecan in Cartilage Lesions Treated with Conditioned Medium of Umbilical Cord Mesencyhmal Stem Cells in Wistar Mice (Rattus novergicus) Soetjahjo, Bintang; Hidayat, Mohammad; Sujuti, Hidayat; Fibrianto, Yuda
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.898 KB) | DOI: 10.11594/jtls.08.01.05

Abstract

Currently, umbilical cord mesenchymal stem cells have the potential to be used as treatment options for any cartilage lesion. This research aimed to evaluate the effects of conditioned medium from umbilical cord mesenchymal stem cells (UC-MSC) on damaged cartilage through the expression of proteins TGF-β1, SOX-9, type II collagen and aggrecan, which are known to be related to chondrogenesis. UC-MSC were isolated from 19-days-pregnant Wistar mice and were cultured using the standard procedure to obtain 80% confluence. Subsequently, the culture was confirmed through a microscopic examination that was driven to be an embryoid body to obtain a pre-condition medium. This research utilized 3-month-old male Wistar mice and was categorized into 6 groups (3 control and 3 treatment groups). Each animal had surgery performed to create a femur condyle cartilage defect. The treatment groups were administered a dose of stem cells at 1 mL/kg. Next, immunohistochemical (IHC) staining was performed to examine the expression of TGF-β1, SOX-9, type II collagen and aggrecan in the 2nd, 3rd, and 4th month of evaluation. The results were analyzed statistically using ANOVA test. For each of the treatment groups, there was increased expression (p < 0.05) in all proteins TGF-β1, SOX-9, type II collagen and aggrecan when compared with control groups at the 2nd, 3rd, and 4th month of evaluation. Pre-conditioned medium from UC-MSC potentially increases the expression of TGF-β1, SOX-9, type II collagen and aggrecan in the damaged cartilage of Wistar mice.
Secondary Knee Osteoarthritis with Medial Instability and Tibial Defect due to Chronic Abscess Treated by Total Knee Arthroplasty with Metal Augmentation - Case Report Soetjahjo, Bintang; Hancoro, Udi Herunefi; Ermawan, Rieva; Saputra, Rhyan Darma; Akbar, Hillan
Indonesian Journal of Medicine Vol 6, No 2 (2021)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background:Knee osteoarthritis (OA), known as degenerative joint disease, is typically the result of wear and tear and progressive loss of articular cartilage, most common in elderly women and men. Knee OA is a leading cause of disability and increase with an aging and overweight population. Total Knee Arthroplasty (TKA) is a common surgical treatment of OA knee that highly cost-effective procedure. Stable placement of components is difficult and thus it presents technical challenges for the surgeon but various options are available for managing these defects.Case Report: A 58 years-old housewife came to our clinic due to chronic left knee pain with associated instability since two years ago and getting worse in last three months. She already had knee joint supplementation injections and physical therapy but no improvements. Based on our clinical and radiological findings, our assessment was secondary left knee osteo-arthritis with medial instability and tibial defect due to chronic abscess. Patient underwent TKA to correct this condition. A revised implant was used to close the defect in the medial part of the tibia and soft tissue reconstruction procedure was used to correct the instability.Result: On the first postoperative month, the patient underwent rehabilitation and physical therapy to strengthen her lower limb muscles. On the second month, she started to walk using walking assisted device. On the ninth months follow-up, the patient was able to walk normally without assisted device, the pain disappeared and the symptoms of knee instability were reduced.Conclusion:Bone defects are commonly encountered during TKA, which still has no standard treatment to cure. In this case, the use of metal augmentation with revision implant give satisfactory results with good clinical outcome.Keywords: Osteoarthritis, Total Knee Arthro¬plasty, knee instability, tibial defect, metal augmentationCorrespondence:Bintang Soetjahjo. Dr. Moewardi Hospital. Jl. Kolonel Sutarto 132, Jebres, Surakarta, Central Java 57126, Indonesia. Mobile: +628122987359. Email: bjortho@yahoo.comIndonesian Journal of Medicine (2021), 06(02): 168-176https://doi.org/10.26911/theijmed.2021.06.02.06
Masalah Perioperatif pada Kasus Emergensi Ortopedik Selama Pandemi Covid-19: Laporan Kasus Amputasi Darurat Pasien Suspek Covid-19 Soetjahjo, Bintang; Hancoro, Udi Herunefi; Ermawan, Rieva; Saputra, Rhyan Darma; Nugroho, Bagus Jati; Abdulhamid, Muhammad
SENADA : Semangat Nasional Dalam Mengabdi Vol. 1 No. 3 (2021): SENADA: Semangat Nasional Dalam Mengabdi
Publisher : Perkumpulan Dosen Periset Indonesia

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Abstract

Penyakit Coronavirus 2019 (COVID-19), yang disebabkan oleh severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), telah menyebar ke seluruh dunia. Selama epidemi COVID-19 yang sedang berlangsung, banyak rumah sakit yang menunda sebagian besar operasi elektif. Namun, beberapa operasi darurat, terutama untuk pasien trauma, tidak bisa dihindari. Untuk pasien yang dicurigai atau dikonfirmasi COVID-19, protokol standar yang terkait persiapan pra operasi, manajemen intraoperatif, dan pengawasan pasca operasi harus diterapkan untuk menghindari infeksi nosokomial dan memastikan keselamatan pasien juga tenaga Kesehatan yang terlibat. Dalam artikel ini kami melaporkan kasus seorang pasien berusia 65 tahun dengan crush injury tungkai bawah dan suspek covid-19. Pasien direncanakan untuk menjalani CITO debridemen dan amputasi di atas lutut. Artikel ini kemudian membahas pertimbangan perioperatif dan teknis yang penting untuk menangani pasien COVID-19 yang membutuhkan perawatan darurat, tanpa mengesampingkan hasil klinis sembari memastikan keselamatan staf yang ada