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Kaban, Joseva Sudiati
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Biochemistry, Genetics & Molecular Biology

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KARAKTERISTIK FISIOLOGIS ENZIM NITRIL HIDRATASE DAN AMIDASE DALAM SEL CORYNEBACTERIURN SP. D5 Sulistinah, Nunik; Kaban, Joseva Sudiati; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3323

Abstract

ABSTRACTPhysiological Characteristics of Nitrile-Hydratase and Amidase From Corynebacteriumsp. D5. Nitrile hydratase (NH-ase) of Corynebacterium sp. D5 is inductive enzyme, butamidase is constitutive enzyme.The best inducer for Nitril hydratase is 2% (vlv) acetonitrille.Nitril hydratase and amidase enzymes showed to be capable of degrading low moleculeweight of aliphatic nitriles and amides. The optimum condition of NH-ase ofCorynebacteriurn sp. D5 were found out at pH 6,6 and 30°C while amidase at pH 7,2 & 50' Crespectively. The inhibitor of both enzymes seemed to be ~ ga'nd H~*'Key words : Nitrile hydratase, bioconversion, Corynebacterium sp. D5, amidase, acetonitrile,aliphatic nitrile
Co-Authors BAMBANG SUNARKO NUNIK SULISTINAH