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All Journal JURNAL TEKNOLOGI LINGKUNGAN Dinamika Pendidikan JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Health Notions ANNALES BOGORIENSES Teknologi Indonesia Performance JOURNAL OF RESEARCH IN MANAGEMENT

BAMBANG SUNARKO

Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Bogor Km 46, Cibinong 16911, Indonesia

Author-ID : 392672

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Penguncilan Gen Penyandi Enzim Nitrilase Enam Isolat Bakteri Unggulan Riffiani, Rini; Sulistinah, Nunik; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Indonesia sebagai negara tropis memiliki biodiversitas yang sangat tinggi. Keanekaragaman hayati ini diperkirakan mencerminkan keanekaragaman kimiawi sekaligus keragaman genetik yang dapat dimanfaatkan untuk mencari biokatalis baru. Enam isolat bakteri yaitu GLB5, LP3, TPIK, MICC, 23A2, dan 23A2 telah diisolasi dari berbagai limbah industri dan mempunyai potensi sebagai pendegradasi nitril.ÃÂ Pengucilan, identifikasi dan purifikasi gen penyandi enzim nitrilase dari keenam isolat bakteri tersebutÃÂ telah dilakukan. Dari kegiatan penelitian ini 3 isolat bakteri unggulan, yaitu GLB5, LP3, dan TPIK teridentifikasi sebagai Rhodococcus pyridinivorans, sedangkanÃÂ MICC teridentifikasi sebagai Bacillus substilis, 23A2 teridentifikasi sebagai Brevibacillus brevis, dan 26A2 teridentifikasi sebagai Microbacterium oxydans. Peta untaian basa nukleutida dari gen penyandi enzim nitrilase dari ketiga isolat yaitu GLB5, LP3, dan TPIK telah terpetakan dengan ukuran gen nitrilase sebesar 960 bp. Hasil analisis dengan BLASTN memperlihatkan bahwa fragmen gen nitrilase yang diamplifikasi dengan primer Nit1101F dan Nit1101R mempunyai homologi yang tinggi terhadap Rhodococcus rhodochrous strain tg1-A6 nitrilase gen dengan persentase kesamaan sebesar 96% .ÃÂ Kata Kunci: Gen, isolasi, nitril, degradasi, enzimÃÂ

Metabolisme Benzonitril oleh Flavobacterium sp. NUB 1 Sulistinah, Nunik; Sunarko, Bambang; Thontowi, Ahmad
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTMetabolism of Benzonitriles by Flavobacterium sp. NUB 1. Flavobacterium sp. NUB 1 was isolated from industrial waste of PT. Petrokimia Gresik. The bacterium was able to utilize benzonitrile and acetonitrile and propionitril as the sole source of carbon and nitrogen. Growth on benzonitrile gave higher growth rate and biomass yield than growth on acetonitrile and propionitrile. When Flavovobacterium sp. NUB1 grew on benzonitril 15 mM , the doubling time is 9 hours 54 minutes and the specific growth rate (?) was 0,07 h-1. Whole cell of Flavobacterium sp. NUB 1 could hydrolyzed aromatic and aliphatic nitriles. The bacteria isolate has ability in metabolism of acetonitrile greater than benzonitrile. Activity of nitrile hydratase and amidase are more dominant than nitrilase in metabolism of benzonitrile.Key words: Biodegradation, benzonitril, Flavobacterium sp. NUB 1, nitrile-hydratase,amidase, nitrilase

Karakteristik Fisiologis Enzim Nitril Hidratase dan Amidase dalam Sel Corynebacteriurn sp. D5 Sulistinah, Nunik; Kaban, Joseva Sudiati; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTPhysiological Characteristics of Nitrile-Hydratase and Amidase From Corynebacteriumsp. D5. Nitrile hydratase (NH-ase) of Corynebacterium sp. D5 is inductive enzyme, butamidase is constitutive enzyme.The best inducer for Nitril hydratase is 2% (vlv) acetonitrille.Nitril hydratase and amidase enzymes showed to be capable of degrading low moleculeweight of aliphatic nitriles and amides. The optimum condition of NH-ase ofCorynebacteriurn sp. D5 were found out at pH 6,6 and 30Â°C while amidase at pH 7,2 & 50 Crespectively. The inhibitor of both enzymes seemed to be ~ gand H~*Key words : Nitrile hydratase, bioconversion, Corynebacterium sp. D5, amidase, acetonitrile,aliphatic nitrile

Karakterisasi Enzim Nitril Hidratase dan Amidase dari Pseudomonas sp. BP3 dalam Biokonversi Adiponitril menjadi Asam Adipat Sunarko, Bambang; Sulistinah, Nunik
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTCharacterization of Nitrile Hydratase and Amidase of Pseudomonas sp BP3 in Bioconversionof Adiponitrile to Adipic Acid. Adipic acid is a commercially important compound, primarilyused as precursor for the production of nylon 6.6. It is also used for plasticizer, fibers, and foodadditive. Synthesis of adipic acid by chemical means requires large amount of energy andconcentrated acid. It also produces N2O as by product, which is very toxic and suspectedcauses depletion of the ozone layer. The purpose of this research was to study thebioconversion of adiponitrile by Pseudomonas sp. BP3 and to characterize the involved enzymesin the whole cell. Pseudomonas sp. BP3 was able to utilize adiponitrile as the sole source ofcarbon and nitrogen. Itâs doubling time (td) and growth rate constant (?) during the growth inadiponitrile were 2 hours and 0.346/h, respectively. The optimum pH and temperature of nitrilehydratasewere pH 7.0 and 30Â°C, respectively, while those of amidase were pH 6 and 50Â°C.Vmax and Ks of nitrile hydratase were 8.3 nM/ml.min. and 55.56 mM, respectively, and ofamidase were 5,9 nM/ml.min and 50 mM. The rate of adiponitrile consumption was 0.245 mM/h and of adipic acid formation was 0.181 mM/h. The yield of bioconversion of adiponitrile andadipamide were about 50 % and 25%, respectively.Key words: Bioconversion, adiponitrile, adipic acid, Pseudomonas sp. BP3, nitrile hydratase,amidase

Pengembangan Sistem Deteksi Senyawa Sianogen dalam Ubi Kayu (Manihot esculenta Crantz) dengan Pendekatan Enzimatis Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.332

Picrate paper test kit for the semiÃ¢â¬âquantitative determination of cyanogenic potential was developed in thisexperiment. The method is relatively simple, easy to use and might be applicable in the field by unskilled person.Paper test was attached on tubes containing sample (100 mg) in aquadest (0,5 mL) and then was immediatelycovered tightly and incubated overnight at room temperature. The colour of picrate paper test changed graduallytowards reddish brown, and its colour was compared with standart colour chart which included 0-800 ppm cyanidethat was also developed in this study. The reddish brown colour of paper test was correlated with cyanideconcentration on the sample. In order to obtain a more accurate detection of cyanogenic compound the paper testwas eluted with 5 mL water or aquadest and the absorbance was measured at 510 nm.Keywords: cyanogenic potential, picrate paper test, semi-quantitative method, simple method, cassava (Manihotesculenta Cranz)

POTENSI Rhodococcus pyridinovorans GLB5 SEBAGAI BIOKATALIS DALAM KONVERSI SENYAWA METHIL SIANIDA DAN PHENIL SIANIDA [Potential of Rhodococcus pyridinovrans GLB5 as Biocatalistin Methyl and Phenyl Cyanide Conversion] Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Nitrile and amide bioconversion have received increasing attention due to their ability to provide a range of commercially important chemicals. The experiment was conducted to investigate the potential of bacterial isolate GLB5 to convert methyl cyanide and phenyl cyanide. The samples were collected from various industrial waste. Selection of isolates to utilize Â these substrates as a sole source of energy, carbon and nitrogen was conducted on 96 whell microtitter plates, based on the growth ability using INT (Iodo nitrotetrazolium chloride) reagent. Based on the growth Â pattern, it showed that the bacterial isolate GLB5 grew well and it was capable of utilizing Â methyl and phenyl cyanide compound as the sole source of carbon and nitrogen. Â The isolate GLB5 was isolated from industrial waste of Batik factory in Cirebon, and Â identified as Rhodococcus pyridinovorans. Bioconversion of methyl cyanide using whole cells of R. pyridinovorans GLB5 showed that ethanamide (C2H5NO) and ethanoic acid (C2H4O2) were detected. Formation of ethanamide and ethanoic acid as the product of bioconversion, indicated that the nitrile hydratase and amidase enzymes Â involved in the bioconversion process. Phenyl carboxamide (C7H7NO) as the product of phenyl cyanide bioconversion was also detected, Â although Â in Â low Â concentration. In this study, R. pyridinovorans GLB5 was capable of completely converting 300 mM methyl cyanide to Â± Â 140 mM ethanoic acid in relatively short times (<60 minutes).

PEMANFAATAN BERBAGAI SENYAWA NITRIL DAN PRODUK DEGRADASINYA SEBAGAI SUBSTRAT UNTUK PERTUMBUHAN ISOLAT BAKTERI TP Supriyati, Dyah; Sunarko, Bambang
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Our experiments showed that bacterial isolate TP was able to grow on acetonitrile, butyronitrile and propionitrile as sole sources of carbon, energy and nitrogen, but not on acrylonitrile dan benzonitrile. Besides on nitriles, isolate TP could grow on acetamide,propionamide, benzamide and nicotinamide, but not on acrylamide. However, none of the tested carboxylic acids could be used as growth subtrate for bacterial isolate TP. The best growth substrates of isolate TP were butyronitrile (CH3-CH2-CH2-CN) and propionamide (CH3-CH2-CONH2). When isolate TP grew on butyronitrile, the highest biomass concentration, the doubling-time (td), and the specific growth rate1(n) were 8.99 gram cell dry weight/liter, 4.8 h and 0.144 h , and when grew on propionamide were 4.57 gram cell dry weight/liter, 5.7 h and10.122 h", respectively.

ISOLASI, SELEKSI, DAN KARAKTERISASI MIKROBA PENDEGRADASI ASETONITRIL DARI LIMBAH INDUSTRI Sunarko, Bambang; Adityarini, Adityarini; Tambunan, Usman Sumo F; Sulistinah, Nunik
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A number of microbes which could grow on acetonitrile were isolated and selected from industrial effluents and were studied to characterise the isolate which has the best degrading capability.Cultures were grown on mineral medium with microelements and acetonitrile was added as sole source of energy, carbon, and nitrogen.Isolate D5, identified as Corynebacteriumsp.,was able to grow on high concentration acetonitrile (up to 5 % v/v) and exhibited the highest specific growth rate (j).When Corynebacterium D5 grew on 2 % (v/v) acetonitrile,the doubling time was 6 hours 40 minutes,the specific growth rate (p) was 0.1 h and the acetonitrile decreasing rate was 3.99 mM/h.Increasing of acetonitrile concentration would extend the doubling time, decline the maximum growth and specific growth rate (i), and biomass production of Corynebacterium 05.The products of acetonitrile degradation by Corynebacterium D5 were acetamide, acetic acid, and ammonia.The maximum growth of Corynebacterium D5 showed when /3-aminopropionitrile was used as a substrate.

PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Sulistinah, Nunik; Sunarko, Bambang
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(j was 6 h 40 minutes, the specific growth rate (J) was 0,046 h the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.

ISOLASI DAN SELEKSI JAMUR PENDEGRADASI SENYAWA BENSONITRIL Subowo, YS; Sunarko, Bambang; Gandjar, Indrawati
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on Isolation and selection of benzonitrile degrading fungi were conducted. The aim of this study was to obtain fungi that high potentially on degrading nitrile compounds. Microbial sources were derivat from industrial wastes. fungi-infecting plantand fungi grown on decayed wood. Eighteen isolates were isolated from those samples. Five isolates were capable to grow on benzonitrile. Isolate-AVI which was identified as Fusarium oxysporum was capable of degrading 0.15% (v/v)Â benzonitrile.

Co-Authors Adityarini, Adityarini AFFANDI, FAKHRUN AFRILIYANI, UCI Agus Suroso Ahmad Thontowi ary yunanto Ashari Ashari Atmosukarto, Ines I. C. Atmosukarto, Ines I.C Cut Misni Mulasiwi Ekaningtyas Widiastuti Febyanti, Alisin Febyanti H. Djoko Windu P. Irawan Indraswati, Denok INDRAWATI GANDJAR Indriati, Suci Ismunarti, Nurbani Aulia Kaban, Joseva Sudiati Kaban, Joseva Sudiati Karsidi Karsidi, Karsidi Matswaya, Akrimi Meyer, Ortwin Munandar, Hendra Nieder, Maria Nofiyanti, Nofa NUNIK SULISTINAH Nur Latifah, Nur Oktafianti, Ika Prasetyoputri, Anggia Putri, Anggi Arinta Putri, Gaviota Gilda RETNO WIDURI Rini Riffiani Sri Martini Subowo, YS Suprijandani Supriyati, Dyah Supriyono, Vincentius Suwaryo, Suwaryo Tambunan, Usman Sumo F Tohir Tohir, Tohir Tuharea, Warda WICAKSONO, BUDIAWAN AJI Wijono, Trimawan Heru Wulandari, Ega Surya Yuliaty, Neti

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