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All Journal JURNAL TEKNOLOGI LINGKUNGAN Dinamika Pendidikan JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Health Notions ANNALES BOGORIENSES Teknologi Indonesia Performance JOURNAL OF RESEARCH IN MANAGEMENT

BAMBANG SUNARKO

Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences-LIPI, Jalan Raya Bogor Km 46, Cibinong 16911, Indonesia

Author-ID : 392672

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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ANALYSIS OF THE EFFECT OF THE IMPLEMENTATION OF TQM ON QUALITY COSTS SUNARKO, BAMBANG; WICAKSONO, BUDIAWAN AJI
JOURNAL OF RESEARCH IN MANAGEMENT Vol 2, No 1 (2019)
Publisher : INDONESIAN RESEARCH SOCIETY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32424/jorim.v2i1.60

The research objective is to analyse the influence of focus on customers, obsession withquality, education and training, benchmarking, teamwork and continuous improvement inquality costs. The hypothesis proposed is Customer Focus, obsession with Quality,Education and Training, Benchmarking, Teamwork and Continuous Improvement affectquality costs. The number of respondents taken in this study was 80 respondents. Theanalytical tool used is using the SPSS application program with techniques. Multipleregression analysis and hypothesis testing using f and t-test.

THE IMPACT OF LONG TERM RELATIONSHIP, PROCESS INTEGRATION, COOPERATION ON SUPPLY CHAIN MANAGEMENT PERFORMANCE AND COMPETITIVE ADVANTAGE SUNARKO, BAMBANG; AFRILIYANI, UCI
JOURNAL OF RESEARCH IN MANAGEMENT Vol 2, No 2 (2019)
Publisher : INDONESIAN RESEARCH SOCIETY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32424/jorim.v2i2.64

This research is conducted on Small and Medium Industries of Gumelem Coconut Sugarlocated in Gumelem, Susukan Subdistrict, Banjarnegara Regency. This study takes the title:"The Influence of Long Term Relationship, Process Integration, Cooperation on SupplyChain Management Performance and Its Impact on Competitive Advantage (Study onGumMem Coconut Sugar IKM)". The purpose of this study was to determine the effect oflong term relationships, process integration, cooperation on supply chain managementperformance and its effect on competitive advantage. The population in this study were allsmall and medium-sized industries of coconut sugar gumelem in Gumelem Kulon. Thenumber of respondents taken in this study were 71 respondents. Sample Randon Samplingis used in determining this respondent. Based on the results of research and analysis ofdata using Multiple Regression Analysis and Simple Regression Analysis shows that: (1)Long term relationships have a positive effect on supply chain management performance,(2) Process integration has a positive effect on supply chain management performance, (3)Cooperation has an effect positive for supply chain management performance, (4) Supplychain management performance has a positive effect on competitive advantage. Theimplications of the above conclusions are in an effort to increase customer satisfaction, theproducers should pay attention to various policies relating to long term relationships,process integration, cooperation. The efforts made are to improve the supply chainmanagement performance in full in accordance with the needs, policies, trust, cooperationrelated to customer satisfaction of coconut sugar gumelem. And also in order to maintaintight competition in the industrial world, companies must improve supply chain managementperformance.

A NEW INDIGENOUS CYANOMETHANE-DEGRADING BACTERIUM ISOLATED FROM GOLD MINING WASTE WATER Sunarko, Bambang; Munandar, Hendra; Sulistinah, Nunik
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3807

ABSTRACT The gold mining wastewater effluent is potential source for isolating cyanides-degrading bacteria, since cyanide is commonly used in gold extraction process in the mining industry. An indigenous bacterial strain LP3, capable of growing on and utilizing of a high concentration of cyanomethane (up to 1.0 Molar), could be isolated from Cikotok gold mine effluent. Based on 16S rDNA sequence, the strain was identified as Rhodococcus pyridinivorans. During the growth on cyanomethane (CH3CN), ethanamide (CH3ONH2) and ethanoic acid (CH3COOH) were detected in the growth media, indicating that nitrile hydratase and amidase involved in the metabolism of the substrate. The involvement of both enzymes on the conversion of cyanomethane was also proved by our study on cyanomethane biodegradation using whole cells of R. Pyridinivorans LP3. Besides cyanomethane, the R. pyridinivorans LP3 could also utilize various aliphatic, aromatic, heterocyclic nitriles and amides as growth substrates. Base on these results, R. pyridinivorans LP3 is expected to be used as a potential candidate for biological treatment for cyanide-containing wastes, although further research is still needed, before being applied on a field scale. Keywords: biocatalyst, cyanide degrading bacteria, gold mining, Rhodococcus pyridinivorans LP3

Karakterisasi Enzim Nitril Hidratase dan Amidase dari Pseudomonas sp. BP3 dalam Biokonversi Adiponitril menjadi Asam Adipat Sunarko, Bambang; Sulistinah, Nunik
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTCharacterization of Nitrile Hydratase and Amidase of Pseudomonas sp BP3 in Bioconversionof Adiponitrile to Adipic Acid. Adipic acid is a commercially important compound, primarilyused as precursor for the production of nylon 6.6. It is also used for plasticizer, fibers, and foodadditive. Synthesis of adipic acid by chemical means requires large amount of energy andconcentrated acid. It also produces N2O as by product, which is very toxic and suspectedcauses depletion of the ozone layer. The purpose of this research was to study thebioconversion of adiponitrile by Pseudomonas sp. BP3 and to characterize the involved enzymesin the whole cell. Pseudomonas sp. BP3 was able to utilize adiponitrile as the sole source ofcarbon and nitrogen. Itâs doubling time (td) and growth rate constant (?) during the growth inadiponitrile were 2 hours and 0.346/h, respectively. The optimum pH and temperature of nitrilehydratasewere pH 7.0 and 30Â°C, respectively, while those of amidase were pH 6 and 50Â°C.Vmax and Ks of nitrile hydratase were 8.3 nM/ml.min. and 55.56 mM, respectively, and ofamidase were 5,9 nM/ml.min and 50 mM. The rate of adiponitrile consumption was 0.245 mM/h and of adipic acid formation was 0.181 mM/h. The yield of bioconversion of adiponitrile andadipamide were about 50 % and 25%, respectively.Key words: Bioconversion, adiponitrile, adipic acid, Pseudomonas sp. BP3, nitrile hydratase,amidase

THE IMPACT OF CASH RATIO, DEBT TO EQUITY RATIO, RECEIVABLES TURNOVER, NET PROFIT MARGIN, RETURN ON EQUITY, AND INSTITUTIONAL OWNERSHIP TO DIVIDEND PAYOUT RATIO YUNANTO, ARY; SUNARKO, BAMBANG; AFFANDI, FAKHRUN
JOURNAL OF RESEARCH IN MANAGEMENT Vol 1, No 4 (2018)
Publisher : INDONESIAN RESEARCH SOCIETY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32424/jorim.v1i4.53

The purpose of this research is to know the influence of cash ratio, DER, recivable turnover, NPM, ROE, and institutional ownership on dividend payout ratio at manufacturing company. This research was conducted at manufacturing company listed in BEI period 2011 until 2016. The sampling technique used is purposive sampling, which is a sample of 19 companies. Data analysis in this study using classical test, multiple linear regression analysis, F test, adjusted R square, and t test. From the results of the research is known that receivables turnover, return on equity, and institutional ownership have a significant positive effect on dividend payout ratio. While the rest, cash ratio, DER, and NPM did not significantly affect the dividend payout ratio in manufacturing companies in 2011-2016.

POTENSI Rhodococcus pyridinovorans GLB5 SEBAGAI BIOKATALIS DALAM KONVERSI SENYAWA METHIL SIANIDA DAN PHENIL SIANIDA [Potential of Rhodococcus pyridinovrans GLB5 as Biocatalistin Methyl and Phenyl Cyanide Conversion] Sunarko, Bambang; Riffiani, Rini; Sulistinah, Nunik
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Nitrile and amide bioconversion have received increasing attention due to their ability to provide a range of commercially important chemicals. The experiment was conducted to investigate the potential of bacterial isolate GLB5 to convert methyl cyanide and phenyl cyanide. The samples were collected from various industrial waste. Selection of isolates to utilize Â these substrates as a sole source of energy, carbon and nitrogen was conducted on 96 whell microtitter plates, based on the growth ability using INT (Iodo nitrotetrazolium chloride) reagent. Based on the growth Â pattern, it showed that the bacterial isolate GLB5 grew well and it was capable of utilizing Â methyl and phenyl cyanide compound as the sole source of carbon and nitrogen. Â The isolate GLB5 was isolated from industrial waste of Batik factory in Cirebon, and Â identified as Rhodococcus pyridinovorans. Bioconversion of methyl cyanide using whole cells of R. pyridinovorans GLB5 showed that ethanamide (C2H5NO) and ethanoic acid (C2H4O2) were detected. Formation of ethanamide and ethanoic acid as the product of bioconversion, indicated that the nitrile hydratase and amidase enzymes Â involved in the bioconversion process. Phenyl carboxamide (C7H7NO) as the product of phenyl cyanide bioconversion was also detected, Â although Â in Â low Â concentration. In this study, R. pyridinovorans GLB5 was capable of completely converting 300 mM methyl cyanide to Â± Â 140 mM ethanoic acid in relatively short times (<60 minutes).

ISOLASI DAN SELEKSI JAMUR PENDEGRADASI SENYAWA BENSONITRIL Sunarko, Bambang; Gandjar, Indrawati; Subowo, YS
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on Isolation and selection of benzonitrile degrading fungi were conducted. The aim of this study was to obtain fungi that high potentially on degrading nitrile compounds. Microbial sources were derivat from industrial wastes. fungi-infecting plantand fungi grown on decayed wood. Eighteen isolates were isolated from those samples. Five isolates were capable to grow on benzonitrile. Isolate-AVI which was identified as Fusarium oxysporum was capable of degrading 0.15% (v/v)Â benzonitrile.

PEMANFAATAN BERBAGAI SENYAWA NITRIL DAN PRODUK DEGRADASINYA SEBAGAI SUBSTRAT UNTUK PERTUMBUHAN ISOLAT BAKTERI TP Sunarko, Bambang; Supriyati, Dyah
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Our experiments showed that bacterial isolate TP was able to grow on acetonitrile, butyronitrile and propionitrile as sole sources of carbon, energy and nitrogen, but not on acrylonitrile dan benzonitrile. Besides on nitriles, isolate TP could grow on acetamide,propionamide, benzamide and nicotinamide, but not on acrylamide. However, none of the tested carboxylic acids could be used as growth subtrate for bacterial isolate TP. The best growth substrates of isolate TP were butyronitrile (CH3-CH2-CH2-CN) and propionamide (CH3-CH2-CONH2). When isolate TP grew on butyronitrile, the highest biomass concentration, the doubling-time (td), and the specific growth rate1(n) were 8.99 gram cell dry weight/liter, 4.8 h and 0.144 h , and when grew on propionamide were 4.57 gram cell dry weight/liter, 5.7 h and10.122 h", respectively.

Penguncilan Gen Penyandi Enzim Nitrilase Enam Isolat Bakteri Unggulan Riffiani, Rini; Sulistinah, Nunik; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Indonesia sebagai negara tropis memiliki biodiversitas yang sangat tinggi. Keanekaragaman hayati ini diperkirakan mencerminkan keanekaragaman kimiawi sekaligus keragaman genetik yang dapat dimanfaatkan untuk mencari biokatalis baru. Enam isolat bakteri yaitu GLB5, LP3, TPIK, MICC, 23A2, dan 23A2 telah diisolasi dari berbagai limbah industri dan mempunyai potensi sebagai pendegradasi nitril.ÃÂ Pengucilan, identifikasi dan purifikasi gen penyandi enzim nitrilase dari keenam isolat bakteri tersebutÃÂ telah dilakukan. Dari kegiatan penelitian ini 3 isolat bakteri unggulan, yaitu GLB5, LP3, dan TPIK teridentifikasi sebagai Rhodococcus pyridinivorans, sedangkanÃÂ MICC teridentifikasi sebagai Bacillus substilis, 23A2 teridentifikasi sebagai Brevibacillus brevis, dan 26A2 teridentifikasi sebagai Microbacterium oxydans. Peta untaian basa nukleutida dari gen penyandi enzim nitrilase dari ketiga isolat yaitu GLB5, LP3, dan TPIK telah terpetakan dengan ukuran gen nitrilase sebesar 960 bp. Hasil analisis dengan BLASTN memperlihatkan bahwa fragmen gen nitrilase yang diamplifikasi dengan primer Nit1101F dan Nit1101R mempunyai homologi yang tinggi terhadap Rhodococcus rhodochrous strain tg1-A6 nitrilase gen dengan persentase kesamaan sebesar 96% .ÃÂ Kata Kunci: Gen, isolasi, nitril, degradasi, enzimÃÂ

Metabolisme Benzonitril oleh Flavobacterium sp. NUB 1 Sulistinah, Nunik; Sunarko, Bambang; Thontowi, Ahmad
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTMetabolism of Benzonitriles by Flavobacterium sp. NUB 1. Flavobacterium sp. NUB 1 was isolated from industrial waste of PT. Petrokimia Gresik. The bacterium was able to utilize benzonitrile and acetonitrile and propionitril as the sole source of carbon and nitrogen. Growth on benzonitrile gave higher growth rate and biomass yield than growth on acetonitrile and propionitrile. When Flavovobacterium sp. NUB1 grew on benzonitril 15 mM , the doubling time is 9 hours 54 minutes and the specific growth rate (?) was 0,07 h-1. Whole cell of Flavobacterium sp. NUB 1 could hydrolyzed aromatic and aliphatic nitriles. The bacteria isolate has ability in metabolism of acetonitrile greater than benzonitrile. Activity of nitrile hydratase and amidase are more dominant than nitrilase in metabolism of benzonitrile.Key words: Biodegradation, benzonitril, Flavobacterium sp. NUB 1, nitrile-hydratase,amidase, nitrilase

Co-Authors Adityarini, Adityarini AFFANDI, FAKHRUN AFRILIYANI, UCI Agus Suroso Ahmad Thontowi ary yunanto Ashari Ashari Atmosukarto, Ines I. C. Atmosukarto, Ines I.C Cut Misni Mulasiwi Ekaningtyas Widiastuti Febyanti, Alisin Febyanti Indraswati, Denok INDRAWATI GANDJAR Indriati, Suci Irawan, Djoko Windu P. Ismunarti, Nurbani Aulia Kaban, Joseva Sudiati Kaban, Joseva Sudiati Karsidi Karsidi, Karsidi Matswaya, Akrimi Meyer, Ortwin Munandar, Hendra Nieder, Maria Nofiyanti, Nofa NUNIK SULISTINAH Nur Latifah, Nur Oktafianti, Ika Prasetyoputri, Anggia Putri, Anggi Arinta Putri, Gaviota Gilda RETNO WIDURI Rini Riffiani Sri Martini Subowo, YS Suprijandani, Suprijandani Supriyati, Dyah Supriyono, Vincentius Suwaryo, Suwaryo Tambunan, Usman Sumo F Tohir Tohir, Tohir Tuharea, Warda WICAKSONO, BUDIAWAN AJI Wijono, Trimawan Heru Wulandari, Ega Surya Yuliaty, Neti

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