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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences International Journal of Applied Mathematics, Sciences, and Technology for National Defense
Fendiyanto, Miftahul Huda
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Environmental Science Mathematics Physics

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Specific function of vitamin D in bone Ramadhani, Dennisa Putri; AD, Hammanda; Azzahra, Salsabila; Rizkyadsyah, Akbar; Ulayya, Anis; Fendiyanto, Miftahul Huda
International Journal of Applied Mathematics, Sciences, and Technology for National Defense Vol 2, No 2 (2024): International Journal of Applied Mathematics, Sciences, and Technology for Natio
Publisher : FOUNDAE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58524/app.sci.def.v2i2.347

Abstract

There are two sources of vitamin D, namely from food and sun exposure. The content of vitamin D is given beneficial in bone and mineral metabolism. Vitamin D helps absorption of calcium in the intestines, thereby accelerating bone mineralization. Bone metabolism is a complex process that occurs throughout life. Very high doses of vitamin D can stimulate an increased rate of bone loss. Vitamin D deficiency causes bone demineralization. Which can cause a decrease in calcium absorption. The functions of these metabolites vary widely in vital metabolic pathways. This review aims to determine the specific function of vitamin D in bone. The search for the data base was carried out by the analytical method.  Vitamin D which is absorbed from food or comes from the skin turns into an active form in the form of 1,25- (OH) 2-D3 which will enter the bloodstream, playing an important role in bone and extra-skeletal health.
Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Rice Cultivars: Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Sindiya, Vita; Fendiyanto, Miftahul Huda; Hamim, Hamim; Miftahudin , Miftahudin
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.11

Abstract

Rice cv. Hawara Bunar is a local rice cultivar tolerant to aluminum (Al) and drought stress. However, the cultivar has inferior characteristics, such as a tall habitus and a small number of tillers, making the cultivar agronomically unattractive. Many genes control plant height and tiller number; one is the TAD1 gene. Analysis of gene expression in two contrasting rice cultivars for both characters is a prerequisite for selecting certain genes for gene editing targets. This study aimed to analyze the gene expression of the TAD1 in rice cv. IR64 and Hawara Bunar, and to construct a phylogenetic tree of genes that regulate rice plant height and tiller number. Gene expression analysis was conducted using the qRT-PCR technique, while the phylogenetic tree was constructed based on the Neighbor-Joining method using PAUP4 software. The results showed TAD1 gene expression in the tillering phase of rice cv. Hawara Bunar is higher than the cv. IR64. The gene expression level in both cultivars corresponds to the plant height and tiller number characters in both rice cultivars. Phylogenetic analysis showed that the TAD1 gene clustered with genes that cause the rice to have a tall habitus with few tillers. The results of a transcriptome meta-analysis reinforced the phylogenetic tree, which shows that the TAD1 gene was found in a group of downregulated genes based on the volcano plots. Therefore, the TAD1 gene can be selected as a target gene for editing in rice cv. Hawara Bunar to obtain superior characters.
Co-Authors AD, Hammanda Azzahra, Salsabila Hamim Hamim Miftahudin , Miftahudin Ramadhani, Dennisa Putri Rizkyadsyah, Akbar Sindiya, Vita Ulayya, Anis