Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

1.829

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Microbiology Indonesia Jurnal Mikologi Indonesia Jurnal Aplikasi Teknologi Pangan MITRA: Jurnal Pemberdayaan Masyarakat

Anastasia Tatik Hartanti

Fakultas Teknobiologi, Universitas Katolik Indonesia Atma Jaya, Jalan Cisauk, BSD City, Tangerang, Banten, 15345

Author-ID : 1769708

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health

Published : 11 Documents Claim Missing Document

Claim Missing Document

Articles

Title

Genotypic Characterization of Rhizopus Spp. Tempeh and Usar: Traditional Inoculum of Tempeh in Indonesia tati barus; Jason Wiranata Sanjaya; Anastasia Tatik Hartanti; Adi Yulandi; Vivitri Dewi Prasasty; David Tandjung
Microbiology Indonesia Vol. 14 No. 3 (2020): September 2020
Publisher : Indonesian Society for microbiology

Abstract. Soybeans tempeh (tempeh) is processed by fermentation using Rhizopus spp. Tempeh is an important source of protein in Indonesia. The traditional inoculum in fermentation locally is known as Usar which is made from the leaves of Hibiscus tiliaceus. However, Rhizopus information from Usar is still limited. Therefore, this study aims to identify and investigate the genetic diversity of Rhizopus species from Usar and tempeh based on the Internal Transcribed Spacer (ITS) sequence and the Random Amplified Polymorphic DNA (RAPD) markers. Twenty-three Rhizopus strains were isolated from Usar and ten Rhizopus strains were isolated from tempeh. Based on ITS sequences, the isolates were similar to R Rhizopus microsporus (30 isolates) and Rhizopus delemar (3 isolates) with 98-99% similarity. The genetics of R. microsporus and R. delemar are varied and different from the genetics of R. microsporus from tempeh. The growth temperature of R. microsporus varies from 33 to 48Â°C and R. delemar can grow to a maximum at 33Â°C. The role of R. microsporus and R. delemar from Usar in determining the quality of tempeh is still limited. Therefore, it needs to be investigated further.

Molecular Diversity of Mold Associated with Gatotan Steffanus Pranoto Hallis; Anastasia Tatik Hartanti; Agustin Wydia Gunawan
Microbiology Indonesia Vol. 15 No. 1 (2021): March 2021
Publisher : Indonesian Society for microbiology

Gatotan is a raw material to make gatot, an Indonesian fermented food, especially in Central Java, made from cassava tubers. Traditionally, the cassava tubers are sun-dried for several days until the black color appears. However, natural fermentation allowed by many types of microorganisms, especially mold in this process could raise concerns about the food safety issues. Previously, the identifications of molds in gatotan were based on morphological observation. Here, we reported the diversity of molds associated with gatotan using molecular identification method. The molecular identification was based on ribosomal DNA internal transcribed spacer (ITS) amplification sequences using combination of ITS4 and ITS5 primers. A total of ten molds were isolated and phylogenetic trees were constructed based on ribosomal DNA sequences. Our results showed that the molds were classified into Lasiodiplodia spp., Trichoderma sp., Aspergillus nomius, Fusarium oxysporum, and Cladosporium sphaerospermum.

Optimization of Rhizopus spp. Production as Mycoprotein using Soymilk Media Dani Muliawan Halim; Anastasia Tatik Hartanti; Stephanie
Microbiology Indonesia Vol. 16 No. 1 (2022): March
Publisher : Indonesian Society for microbiology

Mycoprotein is food with high protein content, fiber, and low in cholesterol made from fungal mycelium. In this research, mycoprotein was produced by spp. isolated fr Rhizopus om tempeh with soymilk as growth media. This research aims to determine the best strain of spp. and op Rhizopus timum carbon to nitrogen ratio for mycoprotein production Two parameters were applied which w . ere inoculum selection and carbon to nitrogen ratio treatment in media. The best inoculum was selected from four strains of Rhizopus spp., ATH 1,ATH 24,ATHÂ 40, and ATH 53. On the other hand, carbon to nitrogen ratio treatment used were as follows 20:1, 20:2, and 40:2. Mycelium dry weight and protein content were measured, as well as reduction sugar, dissolved protein and total volatile base nitrogen concentration in media. The best strain for producing biomass was ATH 24 with 0.6 g of 0 mycelium dry weight per 50 mL of media and the protein content was 0.236 g. The best carbon to nitrogen ratio treatment was 20:1 with 0.57 gram of mycelium dry weight per 50 mLof media and the protein content was 0.20 g. Thus, our data indicate that strain ATH 24 with 20:1 of carbon to nitrogen ratio in media were highly potential for producing mycoprotein. Keywords: fermentation, mycelium, mycoprotein, Rhizopus, soymilk

Co-Authors Adelyne Hanggopertiwi ADI YULANDI Agustin Wydia Gunawan Agustin Wydia Gunawan Agustin Wydia Gunawan Amelia Raharjo Benedicta Evienia Prabawanti Dani Muliawan Halim David Tandjung Dika Putri Salim Dionysius Subali Fransiska Maya Gayuh Rahayu Iman Hidayat Jason Wiranata Sanjaya Meda Canti Steffanus Pranoto Hallis Stephanie Tati Barus Vivitri Dewi Prasasty Warjoto, Renna Eliana

Title Search

Found 3 Documents Search Journal : Microbiology Indonesia

Abstract

Abstract

Abstract

Title

Found 3 Documents
Search
Journal : Microbiology Indonesia