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All Journal Majalah Kedokteran Gigi Indonesia Indonesian Journal of Dental Medicine
Noor, Tengku Natasha Eleena binti Tengku Ahmad
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Dentistry

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Viability of 7F2 pre-osteoblast after Sulawesi stingless bee (Tetragonula biroi) 1% propolis nanoemulsion extraction Sitalaksmi, Ratri Maya; Amalia, Nadya Rafika; Nugraha, Alexander Patera; Budhy, Theresia Indah; Ramadhani, Nastiti Faradilla; Situmorang, Putri Cahaya; Noor, Tengku Natasha Eleena binti Tengku Ahmad; Shariff, Khairul Anuar
Majalah Kedokteran Gigi Indonesia Vol 10, No 2 (2024): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.98860

Abstract

Produced by a stingless bee (Tetragonula biroi) from Sulawesi Island, propolis is an active compound that requires further studies to determine its herbal-based medicinal applications. In addition, propolis is well known as a potential natural ingredient for herbal medicine with minimal side effects. The stingless honeybee, native to Sulawesi, produces propolis, a resin. Propolis from Trigona stingless bees in Sulawesi has the highest flavonoid content compared to Apis mellifera bees. Flavonoids are the most common compounds contained in propolis. Propolis from stingless bees may have the potential to stimulate osteoblast cell proliferation and be responsible for bone regeneration. The objective of this study is to investigate the viability of the 7F2 pre-osteoblast cell line after administration of the stingless bee’s 1% propolis nanoemulsion extract (PNE). A 1% PNE was formulated by maceration methods and diluted into several concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78%. A particle size analysis was done to examine the particle size and zeta potential of the 1% PNE. A Tetrazolium Technique assay was carried out to examine 1% PNE application on a 7F2 pre-osteoblast cell line on days 1, 3, and 7. The highest viability of 7F2 pre-osteoblast cells was at 0.78% concentration on day 7, and the lowest viability was observed at 100% concentration on day 1, with significant differences among groups (p < 0.05). A 1% PNE with a particle size of 151.28 to 182.2 nm and a zeta potential of −32.76 mV was successfully formulated. The 1% PNE was non-toxic to 7F2 pre-osteoblast cells, with a cell toxicity limit at a concentration of 1.56%.
Knowledge Enhancement about Preventive Orthodontic Treatment for Malocclusion and Stunting in Elementary School Children after Community Empowerment Nugraha, Alexander Patera; Narmada, Ida Bagus; Noor, Tengku Natasha Eleena binti Tengku Ahmad
Indonesian Journal of Dental Medicine Vol. 6 No. 2 (2023): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v6i2.2023.66-69

Abstract

Background:  Nutritional insufficiency and stunting during infant growth and development also causes a delay in the ossification centers and may lead to poor skeletal and dental forms resulted in malocclusion. Early interceptive orthodontics can eliminate or reduce the severity of a developing malocclusion, the complexity of orthodontic treatment, the overall treatment time, and the cost. Proper oral and dental health knowledge and a favorable attitude toward oral and dental health are critical in preventing numerous dental issues such as malocclusion. Purpose: the aim of this community empowerment is to enhance the knowledge of preventive orthodontic treatment for malocclusion and stunting in elementary school children. Methods:  The data was collected at Miftahul Ulum Melirang's Madrasah Ibtidaiyah, or elementary school, in Melirang Village, Bungah District, Gresik, East Java. For the community empowerment program assessment, participants were requested to fill out a Google form for the pre- and post-test to identify the enhancement of knowledge about or preventative orthodontic treatment for malocclusion and stunting in primary school children. Results: The majority of the community empowerment participants understood the presentation on oral and dental health empowerment. The post-test percentage was greater (91.5%) than the pre-test rate (53.75%). Conclusion:  The dental and oral health empowerment program about preventive orthodontics, malocclusion, and stunting was effectively completed, enhancing the knowledge level of elementary school children.
Dental caries status among elementary students at Medowo III Elementary School, Kandangan, Kediri, East Java Pramusita, Adya; Triwardhani, Ari; Putranti, Nurul Aisyah Rizky; Rahmawati, Dwi; Nugraha, Alexander Patera; Devitha, Maria; Megantara, Riizko Wira Artha; Noor, Tengku Natasha Eleena binti Tengku Ahmad
Indonesian Journal of Dental Medicine Vol. 7 No. 1 (2024): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v7i1.2024.1-5

Abstract

Background: Dental caries is frequently observed in children. Increasing the awareness of oral health among school-age children is anticipated to decrease the occurrence of dental caries. Purpose: The aim of this study was to determine the prevalence of dental caries among students in Medowo III elementary school using dmft/DMFT index. Methods: A cross-sectional descriptive study was carried out among the entire student at Medowo III elementary school. The dental caries of each student were assessed using the dmft and DMFT indices. The assessment of variable distribution was conducted using the Shapiro-Wilk test. The dmft/DMFT values were compared between different age groups and genders using either the Student's t-test or the Mann-Whitney test. The data was processed using a significance level of p < 0.05. Results: The study included a total of 43 students. The average caries index in primary dentitions was markedly greater than in permanent dentitions. According to WHO, the prevalence of dental caries was very high in the primary dentition of students aged 7-9 years. No notable disparities were detected between female and male students in relation to the caries index of both primary and permanent teeth. However, the average caries index of primary teeth was considerably higher in middle-childhood students compared to preadolescence students. Conclusion: The dental caries prevalence among students in Medowo III elementary school were still high. Therefore, oral health prevention program was needed in order to enhance the community's awareness toward dental caries.
Co-Authors Alexander Patera Nugraha Amalia, Nadya Rafika Ari Triwardhani DWI RAHMAWATI Ida Bagus Narmada Maria Devitha, Maria Megantara, Riizko Wira Artha Nastiti Faradilla Ramadhani Pramusita, Adya Putranti, Nurul Aisyah Rizky Ratri Maya Sitalaksmi Shariff, Khairul Anuar Theresia Indah Budhy Sulisetyawati