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PENGARUH PEMBERIAN DOSIS DOLOMIT TERHADAP PERTUMBUHAN DAN HASIL TANAMAN JAGUNG (Zea mays. L) DI LAHAN GAMBUT Netti Herawati; Nasrez Akhir; Tasya Noor Aina; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 25, No 2 (2023): edisi April
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i2.2756

This research is about the effect of dolomite dosing on the growth and yield of maize (Zea mays L.) on peat soil. This research aimed to obtain the best dose of dolomite on the growth and yield of maize (Zea mays L.) on peat soil. This research was conducted at Matobe Village, Hamlet of Sosoroat, South Sipora District, Mentawai Island. This study used the experimental method in a Randomized Block Design (RBD) with 3 levels of treatment (without using dolomite; 2 ton/ha dolomite; 4 ton/ha dolomite) so that 9 experimental units were obtained. The research data were analyzed statistically using F-test at 5% level. If F count is greater than F table 5%, then proceed with Duncan’s New Multiple Range Test (DNMRT) at the 5% real level. The result showed that the best dose of dolomite on the growth and yield of maize (Zea mays L.) on peat soil was 2 ton/ha dolomite which significantly affected plant height, cob diameter, and the amount of line in each cob.INTISARIPenelitian ini tentang pengaruh pemberian dosis dolomit terhadap pertumbuhan dan hasil tanaman jagung (Zea mays) di lahan gambut. Penelitian ini bertujuan untuk mendapatkan dosis terbaik dari kapur dolomit terhadap pertumbuhan dan hasil tanaman jagung di lahan gambut. Penelitian ini dilaksanakan di Desa Matobe Dusun Sosoroat, Kecamatan Sipora Selatan, Kabupaten Kepulauan Mentawai. Penelitian ini menggunakan metoda percobaan dalam Rancangan Acak Kelompok (RAK) dengan 3 taraf perlakuan (tanpa pemberian kapur dolomit; 2 ton/ha kapur dolomit; 4 ton/ha kapur dolomit) sehingga didapatkan 9 satuan percobaan. Data hasil penelitian dianalisis secara statistik dengan uji F pada taraf 5%. Jika F hitung lebih besar dari F tabel 5%, maka dilanjutkan dengan uji Duncan’s New Multiple Range Test (DNMRT) pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa dosis kapur dolomit terbaik untuk tanaman jagung (Zea mays L.) di lahan gambut adalah dosis 2 ton/ha yang berpengaruh nyata terhadap tinggi tanaman, diameter tongkol, dan jumlah baris per tongkol

PENGARUH DOSIS ARANG AKTIF TERHADAP PERKECAMBAHAN EMBRIO SOMATIK KEDELAI (Glycine max L.) Gustian Gustian; Etti Swasti; Nandita Samaralya Tori; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 25, No 4 (2023): edisi Oktober
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i4.3561

Soybeans (Glycine max L.) are a type of leguminous food crop that is important as a source of protein and vegetable oil, making them a good potential for human growth. Efforts that can be made to increase production and productivity while reducing imports and soybean development include the assembly of superior varieties through tissue culture, with regeneration through somatic embryogenesis. Somatic embryos that have been produced are matured to obtain seedlings by adding activated charcoal. This research aims to determine the effect and the appropriate dosage of activated charcoal for the germination of somatic embryos of soybeans. The research was conducted in the Tissue Culture Laboratory of the Faculty of Agriculture, Andalas University, Padang, from May to August 2023. The research was arranged in a completely randomized design (CRD) with five treatment levels of activated charcoal dosage, namely 1, 1.5, 2, 2.5, and 3 g/l. The data were analyzed using a 5% level F test, and if the calculated F was greater than the Pr(>F), it was followed by a post hoc test using Duncan's Multiple Range Test (DMRT) at a 5% significance level. The results of the research show that the addition of activated charcoal to the media can germinate somatic embryos of soybean plants. In the media containing 2 g/l of activated charcoal, seedlings emerged at 27.15 days after sowing (DAS), and 96.67% of somatic embryos germinated. Keywords: Activated charcoal, germination, somatic embryo, soybean INTISARIKedelai (Glycine max L.) merupakan tanaman pangan jenis polong polongan yang penting sebagai sumber protein dan minyak nabati, sehingga memiliki potensial yang baik untuk pertumbuhan tubuh manusia. Upaya yang dapat dilakukan untuk meningkatkan produksi dan produktivitas dalam menekan jumlah impor dan pengengembangan kedelai yaitu dengan perakitan varietas unggul melalui kultur jaringan yang regenerasinya melalui embriogenesis somatik. Embrio somatik yang telah dihasilkan dilakukan maturasi untuk mendapatkan kecambah dengan metode penambahan arang aktif. Penelitian ini bertujuan untuk mengetahui pengaruh dan dosis arang aktif yang tepat untuk perkecambahan embrio somatik kedelai. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Fakultas Pertanian Universitas Andalas Padang, pada Mei sampau Agustus 2023. Penelitian disususn dalam Rancangan acak lengkap (RAL) dengan 5 taraf perlakukan dengan dosis arang aktif 1, 1,5, 2, 2,5, dan 3 g/l. Data dianalisis menggunakan uji F taraf 5%, jika F hitung lebih besar dari Pr(>F) maka dilanjutkan dengan uji lanjut dengan menggunakan Duncan Multipel Range Test (DMRT) pada taraf 5%. Hasil penelitian menunjukan bahwa pemberian arang aktif pada media dapat mengecambakan embrio somatik tanaman kedelai. Pada media yang mengandung 2 g/l arang aktif menunjukan waktu muncul kecambah pada 27,15 HST dan 96,67% embrio somatik berkecambah. Kata Kunci: Arang aktif, perkecambahan, embrio somatik, kedelai

Hubungan Pemahaman Tentang Bioteknologi Dengan Strategi Coping Stress Mahasiswa Tadris Biologi IAIN Kerinci Dalam Menerima Vaksin Covid-19 Silvia Permata Sari; Dharma Ferry; Emayulia Sastria
Symbiotic: Journal of Biological Education and Science Vol. 4 No. 1 (2023): Symbiotic: Journal of Biological Education and Science Vol. 4 No. 1 April 2023
Publisher : Fakultas Tarbiyah dan Ilmu Keguruan, Institut Agama Islam Negeri Kerinci

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32939/symbiotic.v4i1.100

The government's plan to vaccinate the Indonesian population for Covid-19 has encountered obstacles due to the emergence of hoaxes regarding the Covid-19 vaccine. Several students of Biology Department of IAIN Kerinci who have contracted biotechnology courses, that some of these students have not dared to be vaccinated against Covid-19 because they were affected by the Covid-19 vaccine hoax. The research was conducted on students who had contracted biotechnology courses at the Biology Department of IAIN Kerinci, using quantitative methods and correlational approaches. From the results of the study, it was found that the level of understanding of students' biotechnology was in a low category, namely 42.85% with a frequency of 27 respondents. Meanwhile, students' stress coping strategies in receiving the Covid-19 vaccine were in the high category, namely 84.12% with a frequency of 53 respondents. The results of the correlation analysis show a significance value of 0.007 and it means that there is a relationship between the two variables in this study, but the interpretation of the correlation is categorized as low with a correlation coefficient value of 0.335. This means that increasing understanding of biotechnology will affect the improvement of students' stress coping strategies in receiving the Covid-19 vaccine.

PERTUMBUHAN DAN HASIL TANAMAN SELADA MERAH (Lactuca sativa L.) DENGAN PEMBERIAN FUNGI MIKORIZA ARBUSKULA PADA ULTISOL Netti Herawati; Musliar Kasim; Ririn Anggraini; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 26, No 1 (2024): Edisi APRIL
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i1.4447

Red lettuce is a horticultural crop that has high economic value. Besides being a consumption material, red lettuce is also beneficial for health because it contains anthocyanins. Red lettuce cultivation is still constrained by the large amount of marginal land in Indonesia. Therefore, innovation in red lettuce cultivation is needed through the use of arbuscular mycorrhizal fungi (AMF). Arbuscular mycorrhizal fungi will symbiotize with plant roots and help them absorb nutrients and water in the soil. The purpose of this study was to determine the effect of FMA on the growth and yield of red lettuce and to determine the best dose of FMA for plants. This research was conducted from January to March 2023 at the Wire House of the Faculty of Agriculture, Universitas Andalas Padang. This study consisted of 4 treatments: 0 g/plant, 5 g/plant, 10 g/plant, and 15 g/plant. The results showed that the treatment influenced the parameters of plant height, longest leaf length, number of leaves, leaf area, stem diameter, plant fresh weight, percentage of colonized roots, and net assimilation rate. The conclusion of the research is that the application of FMA can increase the growth and yield of red lettuce plants, to increase the growth and yield of red lettuce plant is sufficient by giving a dose of FMA 5 g/plant. Keywords: arbuscular mycorrhizal fungi, marginal land, red lettuce INTISARISelada merah merupakan tanaman hortikultura yang memiliki nilai ekonomis yang tinggi. Selain menjadi bahan konsumsi, selada merah juga bermanfaat bagi kesehatan karena mengandung antosianin. Budidaya selada merah masih terkendala dengan banyaknya lahan marginal di Indonesia. Fungi mikoriza arbuskular akan bersimbiosis dengan akar tanaman dan membantunya menyerap unsur hara dan air dalam tanah. Oleh karena itu, dibutuhkan inovasi budidaya selada merah melalui penggunaan Fungi Mikoriza Arbuskular (FMA). Tujuan penelitian ini adalah mengetahui pengaruh pemberian FMA terhadap pertumbuhan dan hasil selada merah serta menentukan dosis FMA terbaik bagi tanaman. Penelitian dilaksanakan pada Januari – Maret 2023 di Rumah Kawat dan Laboratorium Fisiologi Tumbuhan Fakultas Pertanian Universitas Andalas Padang. Penelitian ini terdiri dari 4 perlakuan: 0 g/tanaman, 5 g/tanaman, 10 g/tanaman, dan 15 g/tanaman. Hasil penelitian ini menunjukkan bahwa pemberian perlakuan berpengaruh terhadap parameter tinggi tanaman, panjang daun terpanjang, jumlah daun, luas daun, diameter batang, bobot segar per tanaman, persentase akar terkolonisasi FMA, dan laju asimilasi bersih. Dapat disimpulkan bahwa pemberian FMA mampu meningkatkan pertumbuhan dan hasil tanaman selada merah dan untuk meningkatkan pertumbuhan dan hasil tanaman selada merah sudah cukup dengan pemberian dosis FMA 5 g/tanaman. Kata kunci: fungi mikoriza arbuskular, selada merah, tanah marginal.

PENGARUH PEMBERIAN IBA DAN KOMPOSISI MEDIA TUMBUH TERHADAP PEMBENTUKAN AKAR DAN TUNAS SETEK TANAMAN MAWAR (Rosa sinensis) Netti Herawati; Benni Satria; Annisa Najla Salsabila Apri; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 26, No 2 (2024): Edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i2.4694

This research aims to determine the interaction between IBA concentration and growth media composition. Apart from that, determine the provision of IBA and the best composition of growing media for the formation of roots and shoots of rose cuttings. This research was carried out for 4 months, starting from March to June 2022 at the UPT Farm Experimental Garden, Faculty of Agriculture, Andalas University, Padang. The design used was a Factorial Design with 2 factors arranged in a Completely Randomized Design (CRD). The first factor is the provision of IBA which consists of 4 levels (0; 50; 100; 150 ppm). The second factor is the composition of the growing medium which consists of 3 levels: (soil + charcoal husk + cow manure; soil + coconut fiber powder (cocopeat); soil + sand + cow manure). The research data were analyzed statistically using the F test at the 5% level. If the calculated F is greater than the F table of 5%, then proceed with the Duncan's New Multiple Range Test (DNMRT) at level 5. The results of the research show that there is no interaction between the concentration of IBA (Indole-3 butyric acid) and the composition of the growing media in root formation and rose cuttings. The best IBA concentration for forming roots and shoots from rose cuttings is a concentration of 100 ppm, while the best growing medium for forming roots and shoots from rose cuttings is soil + sand + cow manure.

SELEKSI IN VITRO KALUS KEDELAI (Glycine max L.) HASIL INDUKSI MUTASI MENGGUNAKAN ETIL METAN SULFONAT TOLERAN KEKERINGAN DAN ALUMINIUM Gustian Gustian; Muhammad Rafli Ibrahim; Benni Satria; Ryan Budi Setiawan; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 26, No 2 (2024): Edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i2.4695

Soybean (Glycine max L.) is a type of nutritious food crop because soybean seeds have high protein amino acid content. Increasing domestic production for soybean self-sufficiency needs to be done by expanding the planting area. The objective of this research is to examine the effect of ethyl methane sulfonate concentration and obtain soybean plants tolerant to drought and aluminum stress through in vitro selection. This research was conducted from November 2023 to January 2024 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University. The research was arranged in a Completely Randomized Design consisting of 12 treatment levels of the combination of concentrations of abiotic selection agents PEG 4000 and AlCl3 induced by EMS mutation and 12 treatment levels of the combination of concentrations of abiotic selection agents PEG 4000 and AlCl3 not induced by EMS mutation with 3 replications. Data analysis was performed using independent sample t-test at a significance level of 5% and interpreted descriptively. The results showed that mutation induction using ethyl methane sulfonate (EMS) did not produce soybean somatic embryo mutants tolerant to drought and aluminum stress. Meanwhile, results from non-mutated callus produced somatic embryos suspected to be potential putative somaclonal candidates tolerant to drought and aluminum after double in vitro selection using 10% PEG 4000 and 150 ppm AlCl3 selection agents.

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