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All Journal Disease Prevention and Public Health Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Journal Syifa Sciences and Clinical Research (JSSCR) Sasambo Journal of Pharmacy Jurnal Ilmiah Sains Jurnal Yoga Dan Kesehatan Jurnal Novem Medika Farmasi
Hasanuddin, Rusman
Universitas Islam Makassar
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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The Analysis of the Chlorogenic Acid in the Ethanol Fraction of Robusta Coffee Beans and Its Effect on Glucose Levels in Wistar Rats Hasanuddin, Rusman; Jasmiadi, Jasmiadi; Abdillah, Nurliana
Disease Prevention and Public Health Journal Vol 15, No 2 (2021): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v15i2.4705

Abstract

Background: The metabolic disorder caused by high blood glucose levels and pancreatic beta-cell damage is known as diabetes mellitus. Indonesia itself is the 7th country in the world with the number of people with diabetes mellitus. Indonesia occupies rank 7 in the world with the number of people with diabetes mellitus. Apart from that, robusta coffee (Coffea canephora L) is one of the most popular drinks globally, including Indonesia. Chlorogenic acid in coffee beans effectively reduces cell damage due to free radicals, including minimizing excessive glucose release from the liver into the blood. Objective: This study aimed to analyze the effects of chlorogenic acid in the ethanolic fraction of robusta coffee (Coffea canephora L) beans on blood glucose levels in Wistar rats. Method: The researchers applied an experimental study with a randomized pretest-posttest control group design. The beans of robusta coffee were extracted using the Maceration method and then fractionated using a hexane and ethyl acetate solvent. The concentration of the obtained remaining fraction was measured using a spectrophotometer. Furthermore, hyperglycemia testing included 30 Wistar rats induced with 20% glucose for 3 – 4 weeks. They were then given the ethanol fraction of robusta coffee with a dose of 400 mg/kg BW and 500 mg/kg BW. Meanwhile, metformin served as a positive control, and NaCMC served as a negative control. Results: The chlorogenic acid analysis in the ethanol fraction of robusta coffee on a spectrophotometer with a concentration of 37% indicated a decrease of 16.66% on the negative control and 48.06% on the positive control. Meanwhile, the ethanol fraction of each control was 51.53% and 52.16%, respectively. Conclusion: The ethanol fraction of the robusta coffee significantly affects the decrease in blood glucose levels in Wistar rats.
The Analysis of the Chlorogenic Acid in the Ethanol Fraction of Robusta Coffee Beans and Its Effect on Glucose Levels in Wistar Rats Rusman Hasanuddin; Jasmiadi Jasmiadi; Nurliana Abdillah
Disease Prevention and Public Health Journal Vol. 15 No. 2 (2021): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v15i2.4705

Abstract

Background: The metabolic disorder caused by high blood glucose levels and pancreatic beta-cell damage is known as diabetes mellitus. Indonesia itself is the 7th country in the world with the number of people with diabetes mellitus. Indonesia occupies rank 7 in the world with the number of people with diabetes mellitus. Apart from that, robusta coffee (Coffea canephora L) is one of the most popular drinks globally, including Indonesia. Chlorogenic acid in coffee beans effectively reduces cell damage due to free radicals, including minimizing excessive glucose release from the liver into the blood. Objective: This study aimed to analyze the effects of chlorogenic acid in the ethanolic fraction of robusta coffee (Coffea canephora L) beans on blood glucose levels in Wistar rats. Method: The researchers applied an experimental study with a randomized pretest-posttest control group design. The beans of robusta coffee were extracted using the Maceration method and then fractionated using a hexane and ethyl acetate solvent. The concentration of the obtained remaining fraction was measured using a spectrophotometer. Furthermore, hyperglycemia testing included 30 Wistar rats induced with 20% glucose for 3 – 4 weeks. They were then given the ethanol fraction of robusta coffee with a dose of 400 mg/kg BW and 500 mg/kg BW. Meanwhile, metformin served as a positive control, and NaCMC served as a negative control. Results: The chlorogenic acid analysis in the ethanol fraction of robusta coffee on a spectrophotometer with a concentration of 37% indicated a decrease of 16.66% on the negative control and 48.06% on the positive control. Meanwhile, the ethanol fraction of each control was 51.53% and 52.16%, respectively. Conclusion: The ethanol fraction of the robusta coffee significantly affects the decrease in blood glucose levels in Wistar rats.
Isolasi Bakteri Rhizosfer Penghasil Antimikroba Pada Akar Tanaman Jeruk Nipis (Citrus aurantifolia Swingle) rusman hasanuddin
Sasambo Journal of Pharmacy Vol. 1 No. 2 (2020): September
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.244 KB) | DOI: 10.29303/sjp.v1i2.39

Abstract

Isolation of rhizosphere bacteria producing antimicrobial soil around the roots of the lime plant (Citrus aurantifolia Swingle). This study aims to obtain rhizosphere bacterial isolates originating from the soil around the roots of the Lime Plant (citrus aurantifolia Swingle) which can be used as antimicrobials. The research method was taken from the soil around the roots of the Lime plant and then diluted with dilutions of 10-1, 10-2, 10-3 to 10-5 dilution, then poured on NA media containing test microbes. The resulting isolates were then purified and coded JS-1, JS-2, JS-3. Microscopic examination results added 2 gram-negative bacterial isolates namely JS-1 and JS-3, while 1 gram-positive bacterial isolate was JS-2. The resulting isolate was then fermented with NB medium which produced supernatant and biomass, the supernatant was tested antimicrobial which produced active compounds of rhizosphere bacteria (JS-1 and JS-3) from the root soil of the Lime plant have the ability as an antimicrobial activity against Staphylococcus aureus, Escherichia Candida albicans coli and fungal test
Pengaruh Pemberian Hard Candy dari Infusa Kopi Hijau Robusta (Coffea canefora L.) Pada Pasien Diabetes Mellitus Rusman Rusman; Nur Alfiah Irfiyanti
Journal Syifa Sciences and Clinical Research Vol 4, No 2 (2022): Volume 4 Edisi 2 2022
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v4i2.14183

Abstract

a disease with chronic metabolic disorders characterized by high blood glucose levels as a result of insulin resistance is known as diabetes mellitus. Indonesia is among the 7th of the top 10 countries which are estimated to have a diabetes mellitus sufferer of 5.4 million in 2045 and have a low blood sugar control rate. Robusta coffee is the most popular drink, and contains chlorogenic acid, which has anti-diabetic mellitus properties. This study aims to examine the hard candy formulation of robusta green coffee bean infusion (Coffea canephora L) on blood glucose levels and creatinine levels in patients with type-2 diabetes mellitus. the research method was conducted observational, with the free and post design method. This research was conducted at the Pajjaiang clinic in September-October 2020 on prolanis patients diagnosed with type 2 diabetes mellitus. A total of 25 patients were given Hard candy formulation from robusta green coffee bean infusion for 3-4 weeks. The results of the study based on the SPSS data analysis showed that there was an effect on reducing blood glucose levels in diabetes mellitus patients, and the results of the GFR calculation showed no significant effect on creatinine function in diabetes mellitus patients.
Phytochemical Screening, Relationship of Total Phenolic with Antioxidant Activity Of Ethanol and Methanol Extracts of Kesambi (Schleichera oleosa (Lour.) Oken) Bark Nur Alim; Tahirah Hasan; Rusman Rusman; Jasmiadi Jasmiadi; Zulfitri Zulfitri
JURNAL ILMIAH SAINS Volume 22 Nomor 2, Oktober 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.689 KB) | DOI: 10.35799/jis.v22i2.40091

Abstract

Phenolic content has a role in antioxidant activity. The higher the phenolic content of a test sample, the higher the antioxidant activity, which is indicated by a smaller IC50 value. Methanol and 70% ethanol are solvents that have been proven to be widely used to extract total plant phenolic compounds. This study aims to determine the phytochemical content and the relationship of total phenolic content with antioxidant activity of ethanol and methanol extract of Schleichera oleosa (Lour.) Oken) bark using UV-Vis spectrophotometry. Schleichera oleosa (Lour.) Oken was extracted by maceration using 70% ethanol and methanol as solvent. Qualitative analysis of phytochemical compounds using specific reagents, analysis of total phenolic content, and antioxidant activity test using the DPPH method using UV-Vis spectrophotometry at 764 nm and 515 nm, respectively. The results of the qualitative test showed that the ethanol and the methanol extract was positive for alkaloids, flavonoids, and phenolics. The results of the analysis of total phenolic content and antioxidant activity showed that the 70% ethanol extract had a total phenolic content of 7.6829 mg gallic acid equivalent/g with IC50 28.5240 ppm and the methanol extract had a total phenolic content of  9.2057 mg gallic acid equivalent/g with IC50 1.6191 ppm. From these results, it was concluded that the higher the total phenolic content, the higher the antioxidant activity.Keywords: Antioxidant; Schlechera oleosa (Lour.) Oken.; total phenolic Skrining Fitokimia dan Hubungan Kadar Fenolik Total dengan Aktivitas Antioksidan Ekstrak Etanol dan Metanol Kulit Batang Kesambi (Schleichera oleosa (Lour.) Oken)ABSTRAKKadar fenolik memiliki peran terhadap aktivitas antioksidan. Semakin tinggi kadar fenolik suatu sampel uji maka aktivitas antioksidannya juga semakin tinggi, yang ditunjukkan dengan nilai IC50 yang lebih kecil. Pelarut metanol dan etanol 70% merupakan pelarut yang telah terbukti banyak digunakan untuk menarik senyawa fenolik total tanaman.  Penelitian ini bertujuan untuk menentukan kandungan fitokimia, hubungan kadar fenolik total dengan aktivitas antioksidan ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) menggunakan spektrofotometri UV-Vis. Kulit batang kesambi (Schleichera oleosa (Lour.) Oken) diekstraksi dengan cara maserasi menggunakan pelarut etanol 70% dan pelarut metanol. Analisis kualitatif senyawa fitokimia menggunakan pereaksi spesifik, analisis kadar fenolik total dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri UV-Vis masing-masing pada panjang gelombang 764 nm dan 515 nm. Hasil uji kualitatif menunjukkan bahwa ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) positif mengandung alkaloid, flavonoid dan fenolik. Hasil analisis kadar fenolik total dan uji aktivitas antioksidan menunjukkan bahwa ekstrak etanol 70% memiliki kadar fenolik total sebesar 7,6829  mgGAE/g ekstrak dengan nilai IC50 sebesar 28,5240 ppm dan esktrak metanol memiliki kadar fenolik total sebesar 9,2057 mgGAE/g ekstrak dengan nilai IC50 sebesar 1,6191 ppm. Dari hal tersebut disimpulkan bahwa semakin tinggi kadar fenolik total maka semakin tinggi pula aktivitas antioksidannya.Kata kunci: Antioksidan; fenolik total; Schlechera oleosa (Lour.) Oken
Antipyretic Activity of Ethanol Extract from Beligo (Benincasa hispida (Thunb.) Cogn.) Fruit Flesh in Wistar Albino Rats (Rattus norvegicus) Nur Alim; Rusman Hasanuddin; Jasmiadi Jasmiadi; Musdalifah Musdalifah; A. Ihdinal Hagg; Nur Linda
Jurnal Ilmiah Sains Volume 23 Nomor 2, Oktober 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.v23i2.48443

Abstract

The people of South Sulawesi employ beligo as a plant with fever-reducing properties. The utilized part is the flesh of its fruit. The beligo fruit contains fatty acids, flavonoids, phenolics, and steroids that hold promise as antipyretic agents. The study aimed to determine the antipyretic activity of ethanolic extract from beligo fruit flesh on male Wistar rats induced with peptone solution. The research method encompassed maceration extraction using 70% ethanol solvent and antipyretic activity testing using 15 male Wistar rats divided into five treatment groups. Group I served as the negative control and received SODIUM-CMC 1%, groups II, III, and IV received ethanolic extract of beligo fruit flesh at doses of 250 mg/kg BW, 500 mg/kg BW, and 750 mg/kg BW respectively, and group V, the positive control, received paracetamol tablets. Measurements of initial temperature, temperature after induction, and temperature after treatment were taken at 30-minute intervals for 180 minutes. Data were statistically analyzed using a Completely Randomized Design (CRD) and Duncan test. The research findings revealed a fever reduction percentage of 1,57% for group I, 4,03% for group II, 4,28% for group III, 4,45% for group IV, and 5,25% for group V. Data analysis indicated that ethanolic extract of beligo fruit flesh at doses of 250 mg/kg BW, 500 mg/kg BW, and 750 mg/kg BW exhibited significant antipyretic activity (p < 0.05). The ethanolic extract from beligo fruit flesh possesses antipyretic activity in induced Wistar rats, suggesting its potential use as a fever-reducing medication. Keywords: Antipyretic; Benincasa hispida (Thunb.) Cogn; fever
Uji Teratogenik Ekstrak Etanol Biji Beligo (Benincasa Hispida (Thunb.) Cogn.) Pada Mencit Betina (Mus Musculus) Nur Alim; Sri Sulistyawati Anton; Rusman Hasanuddin; Muhammad Ihsan; Ni Ketut Sinarsih
Jurnal Yoga dan Kesehatan Vol 6 No 2 (2023)
Publisher : UHN IGB Sugriwa Denpasar

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Abstract

Selama kehamilan, janin dapat mengalami efek yang tidak dikehendaki dari penggunaan obat herbal dan kimia, terutama pada fase embrionik organogenesisTujuan penelitian ini adalah untuk mengetahui bagaimana biji beligo (Benincasa hispida (Thunb.) Cogn.) mempengaruhi mencit betina (Mus musculus). Penelitian ini melibatkan pengujian teratogenik menggunakan 16 ekor mencit sebagai hewan uji dan ekstraksi melalui maserasi dengan pelarut etanol 70%. Hewan-hewan ini dibagi menjadi 4 kelompok. Pada kelompok perlakuan, ada tiga betina dan satu jantan dalam setiap kelompok. Kelompok 1 kontrol negatif diberi Na-CMC 1%, kelompok II, III, IV diberi ekstrak etanol biji beligo dengan dosis masing-masing 140 mg/kgBB, 280 mg/kgBB dan 420 mg/kgBB. Semua kelompok diberikan sediaan selama hari ke-6 sampai ke-15 masa kebuntingan. Hasil penelitian menunjukkan bahwa ekstrak etanol biji beligo memberikan efek penuh pada fetus mencit. Hasilnya menunjukkan bahwa ekstrak etanol biji beligo dengan dosis 140 mg/kgBB, 280 mg/kgBB, dan 420 mg/kgBB tidak memiliki efek teratogenik pada fetus mencit.
Pengukuran omega-3 pada ikan penja (Awaous sp.) asal Polewali Mandar Provinsi Sulawesi Barat Rusman Hasanuddin; Nur Alim; Karnidayanti Karnidayanti
Sasambo Journal of Pharmacy Vol. 4 No. 2 (2023): September
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/sjp.v4i2.256

Abstract

Omega-3 is an essential fatty acid necessary for human health. It has anti-inflammatory properties, reduces the risk of cardiovascular disease, and is needed for brain development. The three main omega-3 fatty acids are alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Penja fish is a typical marine biota of Polewali Mandar, West Sulawesi, which is believed to be rich in omega-3. The research aimed to determine the omega-3 content in penja fish (Awaous sp.) from Polewali Mandar, West Sulawesi Province. Penja fish was extracted using the Soxhlet method, and then the fatty acid content was analyzed by GC-MS. The results of this study show that penja fish extract (Awaous sp.) contains omega-3 consisting of 2.64% linolenic acid, 0.94% eicosapentaenoic acid (EPA), and 1.17% docosahexaenoic acid (DHA). The total omega-3 content in penja fish (Awaous sp.) is 4.75%.  
Aktivitas Antibakteri Ekstrak Etanol Daging Buah Beligo (Benincasa hispida (Thunb.) Cogn.) terhadap Escherichia coli dan Staphylococcus aureus Rusman Hasanuddin; Nur Alim; Jasmiadi; Ahmad Fauzan
Jurnal Novem Medika Farmasi Vol. 1 No. 3 (2023): Volume 1 Issue 3 April 2023
Publisher : Program Studi Farmasi, Universitas Islam Makassar

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Abstract

Antibacterial is a compound that can be used to inhibit or kill bacteria. Ethanol extract of Beligo fruit flesh [Benincasa hispida (Thunb.) Cogn.)] contains flavanoid compounds that can be used as antibacterials. The purpose of this research was to determine the antibacterial activity of the ethanol extract of beligo fruit flesh [Benincasa hispida (Thunb.) Cogn.)] against bacteria Escherichia coli and Staphylococcus aureus. Research methods include macerated extraction using 70% ethanol and testing antibacterial activity against bacteria Escherichia coli and Staphylococcus aureus with diffusion methods using disc paper. The result obtained using concentration of 12,8%; 25,6%; 51,2% each had an inhibitory diamter of 7,34 mm; 8,97 mm; 12,12 mm for Staphyloccous aureus bacteria while the inhibitory diamter is 6,45 mm; 7,33 mm; 9,91 mm for Escherichia coli bacteria. The conclusion of this research is that the ethanol extract of Beligo fruit flesh has antibacterial activity against Escherichia coli and Staphylococcus aureus.
Isolation Bacteria from the Soil Rhizosphere Robusta Coffee Plant (coffea canephora l.) as an Producer Antibacteria: Isolation Bacteria from the Soil Rhizosphere Robusta Coffee Plant (coffea canephora l.) as an Producer Antibacteria Samsu Hardi; Agus sangka Pratama; Rusman Hasanuddin
Jurnal Novem Medika Farmasi Vol. 1 No. 1 (2022): Volume 1 Issue 1 Agustus 2022
Publisher : Program Studi Farmasi, Universitas Islam Makassar

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Abstract

Rhizosphere soil is the soil around plants that has the same content as the plant where it grows. Much of the Rhizosphere soil content can be used as an antibacterial. The purpose of this study was to isolate bacteria from the rhizosphere soil of robusta coffee plants (Coffea canephora L.) as antibacterial. This research method begins with the isolation of rhizosphere bacteria, purification, antibacterial activity screening test, fermentation antibacterial activity test and thin layer chromatography. The results obtained were in the isolation process, 4 isolates were obtained. Isolate 4 had the greatest activity against Escherhicia coli bacteria, so the next step was used with a concentration of 2.5%; 5%; 10% which have inhibition diameters of 6.23mm, 6.29mm and 6.40mm. The results for the identification of thin layer chromatography of the ethyl acetate extract showed no spots (no compound). Based on these data, it can be concluded that a concentration of 10% has the largest inhibitory diameter against Escherhicia coli bacteria, which is 6.40mm but cannot be identified by the TLC method.
Co-Authors A. Ihdinal Hagg Abdillah, Nurliana Agus Sangka Pratama Agus sangka Pratama Ahmad Fauzan Jasmiadi Jasmiadi Jasmiadi Jasmiadi Jasmiadi, Jasmiadi Karnidayanti Karnidayanti Muhammad Ihsan Muhammad Irhas Musdalifah Musdalifah Ni Ketut Sinarsih Nur Alfiah Irfiyanti Nur Alim Nur Alim Nur Linda Nur Riska Rahma Nurliana Abdillah Samsu Hardi Sri Sulistyawati Anton Tahirah Hasan Zulfitri Zulfitri