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Murhadi .
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Antibacterial Activity Of (Syzygium Polyanta And Amaryllifolius) Leaf Extracts Murhadi .; Susilawati .
Jurnal Teknologi dan Industri Pangan Vol. 18 No. 1 (2007): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The objectives of this research were to study antibacterial activities of syzgium polyanta (“Salam”) and Pandanus amaryllifolius (“Pandan”) leaf extracts and the effect of wet heating (1000, up to 60 min) on their antibacterial activities against staphylococcus aureus, bacillus subtillis, pseudomonas aeruginosa and Escherichia coli. Salam and pandan leaves powder was extracted using hot water (700C, 2 h), ethanol, ethanol/ethylacetate (1:1, v/v), and ethlacetate bt soxhlet (3x8 h) separately. Each residue was further extracted using the same solvent by shaker (250 rpm, 24 h). finally filtrates were mixed and evaporated to produce the extract. Salam leaf ethanol extract (yield 11.50%) showed highest antibacterial activity especially towards P. aeruginosa (diameter of inhibitor 6.5 mm/mg) and B. subtilis (6.3 mm/mg). Pandan leaf erhanol/ethylacetate extract (yield 15.61 %) also showed antibacterial activity towards P. aeruginosa (4.25 mm/mg) and B. subtilis (3.2 mm/mg). In general, salam leaf extracts showed higher antibacterial activity than pandan leaf extracts. Pandan and salam leaf water extracts had no antibacterial activity. Escerichia coli was more resistant to the extracts compared Staphylococcus aureus, bacillus subtilis, and pseudomonas aeruginosa. Antibacterial activity of salam leaf ethylacetate extract decreased 6.55%, lower than that of pandan leaf ethylacetate extract (18.48%) after heating 1000C for 10up to 60 min. Key words : salam, pandan, antibacterial activity.
Spectroscopic Characteristics of Antibacterial Component of Atung (Parinarium glaberrimum Hassk) Seeds Murhadi .; Soewarno T S; Betty S.L Jennie; Anton Apriyantono; Sedarnawati Yasni
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Relative purity assay on antibacterial components (isolates) of “atung” seeds using HPLC system (linier gradient of water-methanol, detection on UV at 280/254 nm) showed that the isolate-9 had the highest purity (94%), followed by isolate-6 (69%), isolate-7 (66%), and isolate-12 (66%) respectively; while the other isolates only had 50% of purity. The UV-Visible spectrophotometer analysis on the isolate-9, showed that the λmax (in methanol) were at 213 and 269 nm. From IR spectrum of the isolate, no more information can be obtained except the presence of C-H stretching. Furthermore, the MS spectrum showed that the fragment ion series of 44 (100%), 57, 69, 83, 97, 115, maybe predicted as aliphatic amine compounds. Key word : Relative purity, methanol, C-H Stretching, fragment ion series, aliphatic amine compounds.
Isolation and Tentative Identification of Volatile Compound of Atung(Parinarium glaberrimum Hassk) Seed Murhadi .; Soewarno T S.; Betty S.L Jennie.; Anton Apriyantono.; Sedarnawati Yasni
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The extraction of volatile components from “atung” seed was carried out by Likens Nickerson extraction apparatus with diethyl eter for 90 minutes, then continued with separation, while volatile isolates identification was done by GC-MS (Shimadzu Qp 5000). The data obtained indicated that volatile components of “atung” seed tentatively are as follow: aldehydes is the largest (34%), alkanes (17%), alkenes (11%), alcohols (7%), and ketones (6%). However, most aldehyde groups were predicted as derivative compounds resulted from the decomposition of fats and/or fatty acids due to a high temperature extraction process. Key words : Volatile components, “atung” seeds, Likens Nickerson, GC-MS, aldehydes.
Co-Authors Anton Apriyantono Anton Apriyantono. Betty S.L Jennie Betty S.L Jennie. SEDARNAWATI YASNI Soewarno T S Soewarno T S. Susilawati .