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Reduksi Gas CO2 oleh Mikroalga Scenedesmus sp. pada Fotobioreaktor Tertutup dengan Variasi Konsentrasi Gas CO2 Nirwawan, Rino; Kussuryani, Yanni; Hanupurti, Dhiti Adiya
Lembaran publikasi minyak dan gas bumi Vol 48, No 1 (2014)
Publisher : PPPTMGB "LEMIGAS"

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (727.201 KB) | DOI: 10.29017/LPMGB.48.1.230

Abstract

Salah satu metode potensial yang dapat digunakan untuk reduksi CO2 adalah memanfaatkan aktivitas mikroalga melalui proses fotosintesis. Mikroalga adalah bioagen yang mampu menangkap CO2 dan mengubahnya menjadi karbohidrat untuk menambah pertumbuhan populasinya. Banyaknya CO2 yang digunakan dapat mencapai hampir dua kali lipat dari berat kering biomassa yang dihasilkan. Tujuan kegiatan ini adalah mengkaji kemampuan mikroalga Scenedesmus sp dalam mereduksi gas CO2 pada suatu fotobioreaktor skala pilot dengan memvariasikan konsentrasi gas CO2 yang diinjeksikan ke dalam sistem. Penelitian dilakukan di Lapangan Gas Subang selama tujuh hari. Komposisi gas CO2 yang digunakan adalah 98%. Sistem operasi adalah sistem batch dan media pertumbuhan yang digunakan adalah media Sederhana 2. Pada penelitian ini digunakan empat rangkaian fotobioreaktor dengan volume operasi masing-masing adalah 60 Liter. Masing-masing fotobioreaktor divariasikan perbandingan jumlah gas CO2 dan udara yang diinjeksikan, yaitu 0:100% (fotobioreaktor 1) yang berfungsi sebagai kontrol, 10:90% (fotobioreaktor 2), 30:70% (fotobioreaktor 3) dan 50:50% (fotobioreaktor 4). Kepadatan sel, optical density (OD), pH, dan berat kering digunakan sebagai parameter pengujian. Hasil penelitian menunjukkan bahwa reduksi gas CO2 tertinggi terdapat pada fotobioreaktor 2 yang terjadi pada hari ke-3 operasi, yaitu sebesar 8,09x10-5 gram dengan nilai kepadatan sel 23,87 x 106 sel/mL. Dari hasil tersebut dapat disimpulkan bahwa penambahan 10% gas CO2 ke dalam fotobioreaktor dapat meningkatkan pertumbuhan mikroalga Scenedesmus sp. One potential method that can be used for the reduction of CO2 is utilizing the microalgae activity through the process of photosynthesis. Microalgae is a bioagen that is able to capture the CO2 and convert it into carbohydrates for the growth of the population. The number of CO2 used can achieve almost double of the dry weight biomass produced. The purpose of this study is to assess the ability of Scenedesmus sp. microalgae in the reduction of CO2 gas at a pilot scale photobioreactor by varying the concentration of CO2 to be injected into the system. The research was done in Subang gas eld for seven days. The composition of the CO2 gas was 98%. The operating system was a batch system and used Sederhana 2as a growth media. In this study we were using four sets of photobioreactor with their respective operating volume of 60 liters. Each photobioreactor has a different ratio of CO2 gas and air to be injected, that is 0: 100% (1st photobioreactor) that serves as the control, 10:90% (2nd photobioreactor), 30:70% (3rd photobioreactor) and 50:50% (4th photobioreactor). Cell density, optical density (OD), pH, and dry weight were used as test parameters. The result showed that the reduction of the highest CO2 gas contained on 2nd photobioreactor which occurs on the 3rd day of the operation, i.e. by 8.09x10-5 gram with cell density of 23.87 x 106 cell/ mL . From these results it can be concluded that the addition of CO2 into the photobioreactor can increase the growth of microalgae Scenedesmus sp.
THE EXTRACTION OF OIL FROM Nannochloropsis sp. MICROALGAE USING ULTRASONIC AND FERMENTATION AS CELL DISRUPTION Hanupurti, Dhiti Adiya; Kristiawan, Onie
Scientific Contributions Oil and Gas Vol 37, No 1 (2014)
Publisher : PPPTMGB "LEMIGAS"

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.37.1.626

Abstract

Algae are a promising source of biofuel but claims about their lipid content can be ambiguous becauseextraction methods vary and lipid quantitation often does not distinguish between particular lipid classes.One of algae types that meet this condition is Nannochloropsis sp. Two different cell disruption methods,i.eultrasonic and fermentation followed by maceration extraction using a mixture of ethanol/hexane (1:1, v/v)as a solvent extraction were studied for their effectiveness in extraction of algae lipids from Nannochloropsissp. Contact time and amplitude were varied in ultrasonic process. Hydrolysis treatment was varied infermentation process, i.e Dillute Acid Pretreatment (DAP) hydrolysis, enzymatic hydrolysis and DAP withenzymatic hydrolysis. The result showed that ultrasonic process followed by maceration extraction wasmore effective for Nannochloropsis sp. lipid extraction. The most ideal treatment was at amplitude 40%and contact time 5 minutes with lipid content of 63.59% of dry weight.