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Early Detection and Serotyping of Dengue Viruses Clinical Isolates Using Reverse Transcription Polymerase Chain Reaction (RT-PCR) 2 Primers Abdul Rahman Siregar; Tri Wibawa; Nastiti Wijayanti
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (620.448 KB) | DOI: 10.22146/ijbiotech.7836

Abstract

Recently several methods for confirming Dengue Virus have been developed involve virus isolation, detection of virus antigen, and nucleic acid using PCR. It has been reported that rapid detection method for confirming DHF by Multiplex RT-PCR had been successfully developed. It was more effective than the other methods with a high sensitivity and specivicity were 100% at the early phase (day 1-3). This study was designed to develop rapid detection and serotyping methods for Dengue Virus using RT-PCR 2 primers (Dcon and preM) with annealing temperature was 57oC. The whole blood samples were collected from suspected dengue fever patients that had been confirmed with NS1 kit from R.S. Persahabatan DKI Jakarta and R.S. Prof. Dr. Sardjito DI Yogyakarta during Februari-August 2009. The PCR products showed that in 12 samples, 100 % were postitive with different pattern among the serotypes especially for DEN1 and DEN2, but not for DEN3 and Den4.  This method was also able to confirm the double infection DEN2-DEN3, but not for the other ones because of the unspecific pattern. From the results, it indicated that the 2 primers can be a promising early detection and serotyping method of Dengue Virus which infected the DHF patients. Key words: Dengue Virus, DHF, early detection, serotyping, RT-PCR 2 primers.
ANALISIS YURIDIS KEWENANGAN OTORITAS JASA KEUANGAN DALAM PENGATURAN DAN PENGAWASAN TERHADAP LEMBAGA PEMBIAYAAN KONSUMEN ABDUL RAHMAN SIREGAR
PREMISE LAW JURNAL Vol 14 (2015): Volume XIV Tahun 2015
Publisher : PREMISE LAW JURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.522 KB)

Abstract

A consumer financial institution is a part of business activities in the industry of financial services and does business activities to finance the provisions of goods based on consumers’ needs as to finance motor vehicles, home equipment, electronic goods and housing. The consumer financial institution in legal institution in Indonesia is arranged in Book III of the Civil Codes on Agreement, Law No.21/2011 on Authority of Financial Service, PMK No.84/PMK.012/206 on Financing Company, Presidential Regulation No.9/2009 on Financing Institution and Regulations of Authority of Financing Service which arrange business  activities of financial institutions. The regulating forms of Authority of Financing Service are the rulings of regulations and policies to business activities to finance consumers and in the form of direct and indirect supervisions of authority of financial service. Breaking the regulations/conditions and the Authority of Financing Service does not fulfill the conditions, the financing company will be given a legal sanction, namely an administrative sanction. Keywords        :                      Authority of Financing Service, Regulating and Supervising, Consumer Financial Institution
Design of Laboratory Scale Oil Distillation Machine Dhimas Satria; Imron Rosyadi; Ade Irman; Rina Lusiani; Haryadi Haryadi; Abdul Rahman Siregar
World Chemical Engineering Journal VOLUME 1 NO. 3 DECEMBER 2016
Publisher : Chemical Engineering Department, University of Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36055/wcej.v1i3.945

Abstract

In Indonesia there are 4,000 old oil wells are not exploited again by Pertamina becauseit is not economical, some old wells exploited and processed in the traditional way.Their traditional oil processing has not significantly give a role to the income of localcommunities, as the mining process that is not managed by modern technologyresulting in a weak bargaining position. This study aims to participate in the welfare ofthe community in areas rich in oil resources and also support the energy security bydeveloping technologies distillationof crude oil into diesel by exploiting oil wellsparents who are not exploited again by Pertamina that will improve the bargainingposition (bargaining power), increase revenue, reduce unemployment and poverty andhas created a system of social safety nets and insurance against society.The methodused is the method of Pahl and Beitz, which consists of the Planning and TaskClarification, Conceptual Design, Embodiment Design, and Detail Design.The results ofthe research are the best variant for the design of machines fractional distillation ofpetroleum laboratory scale variant 1 (Boiler cylinder - 0.5 inch Pipe - Tubes distillationcylinder - gas stove), by reason of the appropriate size pipe diameter and more securethan the variance other variants. While the dimensions obtained from the draft is 1.31m 2 for a total dimension of the tower area (AT) and 408 mm for diameter Total Tower(DT), and 3 mm for the reactor tube wall thickness. In addition, the test results of oilthat has been processed using distillation machine laboratory scale show still makesdiesel specifications in accordance with standards established by the Government,namely the specifications for specific gravity, viscosity, water content, maximumheating and visual appearance.