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All Journal e-GIGI Jurnal MIPA PHARMACON CHEMISTRY PROGRESS
Jemmy Abidjulu
Universitas Sam Ratulangi
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Mathematics Medicine & Pharmacology Physics

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Uji daya hambat ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Candida albicans secara in vitro Simatupang, Olivia C.; Abidjulu, Jemmy; Siagian, Krista V.
e-GiGi Vol 5, No 1 (2017): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.5.1.2017.14701

Abstract

Abstract: Candida albicans is a normal flora of the mouth, respiratory tract, gastrointestinal tract and the female genital but it becomes pathogen if there is a predisposing factor. Approximately 85-95% of oral candida infections are caused by C. albicans that is usually attached to the labial mucosa, buccal mucosa, the dorsal parts of the tongue and palate. Noni (Morinda citrifolia L.) is already known as a medical plant; one of it parts that has a medicinal effect is the leaf that contains anthraquinon as an antifungal. This study was aimed to determine the effect of noni leaf extract to the growth of C. albicans. This was a true experimental in vitro study with the post test only group design. The Kirby-Bauer method with filter paper was used to evaluate the sensitivity of C. albicans to mengkudu leaf extract. Leaves samples were extracted by using maceration process with ethanol 96%. C. albicans obtained from the pure stock of Laboratory of Microbiology Pharmacy Faculty of Math and Science, University of Sam Ratulangi. The results showed that the inhibition zone diameter of noni leaf extract on the growth of C. albicans was 16 mm (strong inhibition category). Conclusion: Noni leaf extract (Morinda citrifolia L.) had an inhibitory effect on Candida albicans.Keywords: Mengkudu leaf (Morinda citrifolia L.), Candida albicans, inhibition zone Abstrak: Candida albicans merupakan anggota flora normal rongga mulut, saluran pernafasan, saluran pencernaan, dan genital wanita namun dapat menjadi patogen jika terdapat faktor predisposisi. Sekitar 85-95% infeksi kandidiasis oral disebabkan oleh C albicans yang biasanya melekat pada mukosa labial, mukosa bukal, dorsum lidah dan palatum. Tanaman mengkudu (Morinda citrifolia L.) telah dikenal sebagai tumbuhan obat. Salah satu bagian tanaman mengkudu yang memiliki efek obat ialah daunnya karena adanya kandungan antrakuinon yang bersifat antijamur. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun mengkudu terhadap pertumbuhan C. albicans. Jenis penelitian ini ialah eksperimental laboratorik secara in vitro dengan post test only group design. Pengujian menggunakan metode Kirby-Bauer berbahan kertas saring. Sampel daun diekstraksi dengan proses maserasi menggunakan etanol 96%. Jamur C. albicans diambil dari stok biakan jamur murni Laboratorium Farmasi FMIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan diameter zona hambat ekstrak daun mengkudu terhadap pertumbuhan C. albicans 16 mm yang tergolong kriteria zona hambat kuat. Simpulan: Ekstrak daun mengkudu (Morinda citrifolia L.) mempunyai daya hambat terhadap jamur Candida albicans.Kata kunci: daun mengkudu, Candida albicans, zona hambat
GAMBARAN KEBOCORAN TEPI TUMPATAN PASCA RESTORASI RESIN KOMPOSIT PADA MAHASISWA PROGRAM STUDI KEDOKTERAN GIGI ANGKATAN 2005-2007 Mukuan, Theo; Abidjulu, Jemmy; Wicaksono, Dinar A.
e-GiGi Vol 1, No 2 (2013): e-GiGi Juli-Desember 2013
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.1.2.2013.2625

Abstract

Abstract: Composite resin is one of the restoration material which has the advantage in aesthetic due to its colour. This make the composite resin as a material of choice, unfortunately most of patients who have received composite resin treatment doesn’t have a proper careness to its own restoration after treatment. Aim: to study how many people who use composite resin as restoration material and to study if the restoration has any leakage after treatment. Results: the study showed that women have more composite resin restoration than men. The leakage occurred to three different subject in this research which is the most leakage happened to 2007’s student, followed by 2006’s and 2005’s. Class one Black classification is the most restoration to be found in this study followed by class 4, class 5, class 3, and class 2. The leakage which found in this study mostly occurred to molar restorations, followed by premolars while incicivus was found to be the least. Keywords: resin, composite, leakage, restoration.   Abstrak: Resin komposit merupakan salah satu jenis bahan tumpatan yang memiliki keunggulan dalam bidang estetik karena merupakan bahan tumpatan yang sewarna dengan gigi. Hal ini menjadikan resin komposit sebagai bahan pilihan restorasi, namun banyak pasien yang telah mendapatkan perawatan restorasi resin komposit kurang memperhatikan tumpatan pasca restorasi tersebut. Tujuan: penelitian ini bertujuan untuk mengetahui gambaran penggunaan resin komposit sebagai bahan tumpatan gigi pada restorasi kavitas dan mengetahui apakah telah terjadi kebocoran tepi tumpatan resin komposit pada perawatan restorasi kavitas. Hasil penelitian: menunjukkan distribusi penggunaan komposit lebih banyak pada perempuan dibandingkan laki-laki. Terjadi kebocoran tumpatan pada subjek penelitian dari tiga angkatan yang berbeda yaitu terbanyak pada mahasiswa angkatan 2007 kemudian 2006 dan 2005. Tumpatan kavitas kelas I merupakan tumpatan yang paling banyak diikuti kelas 4, kelas 5, kelas 3, dan kelas 2. Kebocoran gigi yang ditemukan pada penelitian ini terjadi paling banyak pada gigi molar kemudian premolar sedangkan insisivus paling sedikit. Kata kunci: resin, komposit, kebocoran, restorasi.
Uji daya hambat ekstrak buah pala (myristica fragrans Houtt) terhadap bakteri penyebab periodontitis porphyromonas gingivalis secara in vitro Kaawoan, Pricillia T.; Abidjulu, Jemmy; Siagian, Krista V.
e-GiGi Vol 4, No 2 (2016): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.4.2.2016.13504

Abstract

Abstract: Periodontal disease is preceded by a buildup of plaque that contains a collection of bacteria. The most common bacteria found in plaques are Porphyromonas gingivalis that cause periodontitis. There are several ways to treat periodontitis inter alia the usage of natural materials. Nutmeg (Myristica fragrans Houtt) contains volatile oil, saponins, and alkaloids known as antibacterials. This study aimed to investigate the inhibitory effect of nutmeg extract on Porphyromonas gingivalis. This was an experimental laboratory study in vitro with a post test only control group design. The testing method used in this study was a modified method of Kirby-bauer. Nutmeg was extracted by using maceration method with ethanol 96%. Porphyromonas gingivalis bacteria were ordered from University of Hasanuddin Makassar and then were rejuvenated in the Laboratory of Microbiology Pharmacy FMIPA University of Sam Ratulangi Manado. The results showed that the average inhibitory zone of nutmeg extract was 13,5 mm. Conclusion: Nutmeg (Myristica fragrans Houtt) extract had an inhibitory effect on the Porphyromonas gingivalis bacteria.Keywords: nutmeg (Myristica fragrans Houtt), inhibition zone, periodontitis, Porphyromonas gingivalisAbstrak: Penyakit periodontal berawal dari penumpukan plak yang mengandung kumpulan bakteri. Bakteri yang paling banyak ditemukan yaitu bakteri Porphyromonas gingivalis yang menyebabkan penyakit periodontitis. Terdapat beberapa cara untuk mengobati periodontitis, salah satunya dengan penggunaan bahan alami. Pala (Myristica fragrans Houtt) memiliki kandungan minyak atsiri, saponin, dan alkaloida yang diketahui berefek antibakteri. Penelitian ini bertujuan untuk mengetahui adanya daya hambat ekstrak buah pala terhadap bakteri penyebab periodontitis Porphyromonas gingivalis. Jenis penelitian ini ialah eksperimental laboratorik secara in vitro dengan post test only control group design. Metode pengujian yang digunakan yaitu modifikasi Kirby-bauer menggunakan sumuran. Sampel buah pala diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Porphyromonas gingivalis yang digunakan dalam penelitian ini dikirim dari Universitas Hasanuddin Makassar yang telah diremajakan di Laboratorium Mikrobiologi Farmasi FMIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan zona hambat ekstrak buah pala sebesar 13,5 mm. Simpulan: Ekstrak buah pala (Myristica fragrans Houtt) mempunyai daya hambat terhadap bakteri penyebab periodontitis Porphyromonas gingivalis.Kata kunci: pala (Myristica fragrans Houtt), zona hambat, periodontitis, porphyromonas gingivalis
AKTIVITAS ANTIOKSIDAN DARI EKSTRAK KULIT NANAS (ANANAS COMOSUS (L) MERR) Hatam, Sri Febriani; Suryanto, Edi; Abidjulu, Jemmy
PHARMACON Vol 2, No 1 (2013): pharmacon
Publisher : PHARMACON

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACT The objectives of this research were to determine antioxidant n activity from pineapple peal extract. Pineapple peel extracted by maceration, soxhlet and reflux using ethanol 80% as a solvent. Phenolics and flavonoids content of Crude extract were analyzed.Evaluation of antioxidant activity using DPPH free radicals method, The results shows that maceration, soxhlet and reflux extract possess phenolics content 16,53; 28,78; 16,02 g/mL respectively, and  flavonoid total content 3,514; 5,115; 4,414 g/mL respectively. Maceration extract with concentration 3000 g/mL possess low free radical scavenging activity, and soxhlet extract possess the highest.Inhibition concentration 50% (IC50) of soxhlet extract were 602,56 g/mL, it?s indicate that soxhlet extract possess high scavenging of 50% free radicals. This result shows that pineapple peel extract possess phytochemical phenolics and flavonoids that potential to be antioxidant and as active compound of sun screen. Keywords: antioxidant, pineapple peel extract.
PENGARUH SUHU TERHADAP STABILITAS SERTA PENETAPAN KADAR TABLET FUROSEMIDA MENGGUNAKAN SPEKTROFOTOMETER UV-VIS Waney, Rekanita; citraningtyas, Gayatri; Abidjulu, Jemmy
PHARMACON Vol 1, No 2 (2012)
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.1.2012.504

Abstract

Stability is the ability of product to maintain its nature and characteristics similar to itsorigin during storage and use period. Furosemide is one of drugs product that works as diuretic.The aim of this research was to study the effect of temperature on furosemide stability. Themethod using in this research is factorial Complete Random design. There are two factors in thisresearch,factor A is temperature (40oC, 50 oC, and 60 oC) and factor B are period (60, 120 and180 minutes). There are three samples in this research. Analysis of furosemide content usingSpectrophotometer UV-Vis at λ = 271 nm. Obtained data were analyzed using spss ver. 20. Thedifferences between variable were statistical analyzed. The result shows that the decreasing offurosemide in tablet starts at temperature 50°Cafter60minutes heating for sample A and C.Sample B decrease at temperature 50°C after 120 minutes heating.Keywords: stability, furosemide, spectrophotometry UV-Vis
DAMPAK LIMBAH PETERNAKAN AYAM TERHADAP KUALITAS AIR SUNGAI SAWANGAN DI DESA SAWANGAN KECAMATAN TOMBULU KABUPATEN MINAHASA Olivianti, Asriani; Abidjulu, Jemmy; Koleangan, Harry
CHEMISTRY PROGRESS Vol 9, No 2 (2016)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.9.2.2016.27986

Abstract

ABSTRAK Tujuan dari penelitian ini untuk menganalisis dampak limbah peternakan ayam terhadap kualitas air sungai Sawangan. Lokasi pengambilan sampel dilakukan pada lima titikyaitu pada pipa pembuangan limbah peternakan ayam yang langsung mengarah ke sungai (T3), 25m (T1) dan 10m  (T2)  ke arah hulu sebelum pipa pembuangan serta 10m (T4) dan 25m (T5) ke arah hilir sesudah pipa pembuangan limbah ternak. Hasil penelitian menunjukkan bahwa kualitas air sungai Sawangan yang tidak memenuhi standar baku mutu berada pada T3, semua parameter yang diperika baik pH, nitrit, nitrat, amonium, TDS, TSS, DO, BOD, COD tidak memenuhi ketentuan pemerintah dalam PP No. 82 Tahun 2001 tentang standar kualitas air. Pada lokasi yang lain ( T1, T2, T4 & T5) teramati bahwa kualitas air sungai masih memenuhi ketentuan yang berlaku. ABSTRACTA research had been conducted to study the effect of chicken farming waste on Sawangan river water quality. Sampling location were set at 25 m (T1), 10 m (T2), 0 m (T3) upstream 10 m (T4) and 25 m (T5) downstream. The results showed that at T3, all of the examined parameters pH, nitrite, nitrate, ammonium, TDS, TSS, DO, BOD, COD do not meet the prerequisite ruled by government in PP No. 82 of 2001 about water quality standard. At other locations (T1, T2, T4, & T5), it appears that the river water quality still fulfill the requirement. 
ANALISIS KUALITAS AIR SUNGAI TANOYAN DI KOTA KOTAMOBAGU PROVINSI SULAWESI UTARA Abidjulu, Jemmy
CHEMISTRY PROGRESS Vol 1, No 2 (2008)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.1.2.2008.4960

Abstract

A research has been done about the quality of water from Tonayan river in Kotamobagu city Province ofNorth Sulawesi based on its physics and chemical parameter. The sample was taken on three spot withduplo methods. The first sample was undertaken on the upperstream at Tanoyan village, second wasundertaken on the downstream at Mopait village and the third was undertaken at Kopandakan village.Analysis of water using Turbiditimetry, pH-metri, EDTA, and Spectrophotometer methods. The resultthat had been obtained was compared with the standart of pure water class II based on the governmentrules No.82 year 2001. Based on the research result, the quality of river water at the three spot are: notaste, no flavor, turbidymetry ( 3,1-9 NTU), pH (6,5-7,9), saturated (440-560 mg/1), chloride (0,02-21,02mg/L), ferrum (<0,01-0,036 mg/L), mangannese (0,02-0,071 mg/L), nitrat (<0,5 mg/L), seng (0,02-0,215mg/L), cyanide (0,11-0,01 mg/L), dan sulphat (2-71 mg/L). This research conclude that water inTonayan river Kotamobagu city Province of North Sulawesi for all parameters are in standart quality,accept saturated at first spot and zinc at the third spot Keywords: Quality, water, river, physics parameter, chemical parameter
PENGARUH KONSENTRASI SIANIDA TERHADAP PRODUKSI EMAS Tangkuman, Herling D.; Abidjulu, Jemmy; Mukuan, Hendra
CHEMISTRY PROGRESS Vol 1, No 1 (2008)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.1.1.2008.22

Abstract

The research has been done to study the effect of cyanide concentration on gold production. There are three step to produced gold. First is repulper, second is leaching and the last is burning. The result show that concentration of cyanide give effect in gold production. Cyanide with 100 ppm significantly different compared with cyanide with 200, 400 and 600 ppm in gold recovery. Based on statistical analysis, cyanide with 400 ppm is the best concentration in gold production.
Analisis Fisika-Kimia Air Sumur Di Tempat Pembuangan Akhir Sumompo Kecamatan Tuminting Manado Tambunan, Martin Aprilino; Abidjulu, Jemmy; Wuntu, Audy
Jurnal MIPA Vol 4, No 2 (2015)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.4.2.2015.9114

Abstract

Telah dilakukan penelitian tentang kualitas fisika-kimia air sumur di tempat pembuangan akhir sampah (TPA) kecamatan Tuminting, Manado secara kualitatif dan kuantitatif. Kajian yang dilakukan meliputi parameter fisika yaitu penentuan pH, bau, rasa, warna, kekeruhan dan total padatan terlarut (TDT) serta parameter kimia yaitu penentuan nitrat, nitrit, klorida, sulfat, besi dan mangan. Berdasarkan hasil pengujian parameter fisika pada 4 titik pengambilan air ada beberapa titik pengambilan sampel yang tidak sesuai dengan syarat baku mutu kualitas air yaitu tidak berbau dan berasa serta memiliki rentang pH 6,5-9,2 dan memiliki nilai maksimal untuk warna 50, kekeruhan 25, dan TDT 1500. Begitu juga untuk parameter kimia ada beberapa titik pengambilan sampel yang tidak sesuai dengan syarat baku mutu kualitas air yaitu memiliki nilai maksimal untuk klorida 600 mg/L, nitrat 10 mg/L, nitrit 1 mg/L, besi 1 mg/L, mangan 0,5 m/L dan sulfat 400 mg/L.A research on the physico-chemical quality of the well water in the landfill (TPA) district Tuminting, Manado has been done. The study includedphysical parameters, namely pH, odor, taste, color, turbidity and total dissolved solids (TDS) and chemical parameters, namely nitrate, nitrite, chloride, sulphate, iron and manganese. Among the four sampling sites, some sites did not meet the water quality standard based on some physical parameters, those are odorless and tasteless, pH range of 6.5 to 9.2 and maximum value of 50 for color, 25 for turbidity, and 1500 for TDS. Likewise, for chemical parameters, several sampling points are not in accordance with the terms of water quality standard, those aremaximum value of 600 mg/Lfor chlorides, 10 mg/L for nitrate, 1 mg/L for nitrite, 1 mg/L for iron, 0.5 m/L for manganese and 400 mg/L for sulphate.
Isoterm Adsorpsi Rhodamin B Pada Arang Aktif Kayu Linggua Roring, Stero H.; Pitoi, Mariska M.; Abidjulu, Jemmy
Jurnal MIPA Vol 2, No 1 (2013)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.2.1.2013.764

Abstract

Telah dilakukan penelitian adsorpsi rhodamin B pada arang aktif yang dibuat dari kayu linggua. Penelitian ini bertujuan untuk menentukan koefisien adsorpsi arang aktif limbah gergajian kayu linggua terhadap zat warna rhodamin B dengan menggunakan isoterm adsorpsi Langmuir dan Freundlich. Penelitian dilakukan secara batch di mana 0,1; 0,2; 0,4; 0,7 dan 1 gram arang aktif dimasukkan ke dalam labu Erlenmeyer terpisah yang masing-masing telah berisi 100 mL larutan rhodamin B 100 ppm. Campuran kemudian diperlakukan di atas magnetic stirrer selama 50 menit dan selanjutnya konsentrasi larutan rhodamin B dianalisis menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa isoterm adsorpsi rhodamin B pada arang aktif kayu linggua lebih mengikuti isoterm adsorpsi Freundlich dengan koefisien adsorpsi 2,999x10-4 dan nilai intensitas adsorpsi 1,459.Adsorption isotherm of rhodamine B by activated carbon made from linggua wood waste was investigated. The adsorption isotherm was analyzed by Langmuir and Freundlich models. The isotherm was measured by batch experiment where 0,1; 0,2; 0,4; 0,7; and 1,0 grams where added into the separated Erlenmeyer flasks filled with 100 mL of 100 ppm rhodamine B. The mixtures were then treated on magnetic stirrers for 50 minutes followed by rhodamine B analysis using spectrophotometer UV-Vis. The result showed that the isotherm followed the Freundlich model better than Langmuir with the Freundlich adsorption coefficient of 2,999x10-4 and the Freundlich exponent of 1,459.
Co-Authors Audy D. Wuntu Audy Wuntu Dinar A. Wicaksono Edi Suryanto Erling Pasaraeng Erpi Bangol Gayatri Citraningtyas Ghazaly R. Umaternate Harry S. J. Koleangan Harry S. Koleangan Hendra Mukuan Herling D. Tangkuman Jhon Smith Wongkar, Jhon Smith Koleangan, Harry Krista V. Siagian Lidya I. Momuat Mariska M. Pitoi Martin Aprilino Tambunan, Martin Aprilino Maureen Kumaunang Max R. J. Runtuwene Melin T. Kitong Mercy Ngajow Olivianti, Asriani Pricillia T. Kaawoan, Pricillia T. Rekanita Waney Simatupang, Olivia C. Sitti N. Sultan, Sitti N. Sri Febriani Hatam Stero H. Roring Theo Mukuan Triyono Mojo, Triyono Vanda S Kamu Vanda S. Kamu