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All Journal Jurnal Ilmu Ternak dan Veteriner Journal of the Indonesian Tropical Animal Agriculture
A. Anggraeni
Indonesian Research Institute for Animal Production , Jl. Veteran III, Desa Banjarwaru, Ciawi, Bogor 16002
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Verification for the control of kappa-casein gene on milk protein yield of friesian-holstein in dairy central region of West Java Anggraeni, A.; Sumantri, C.; Farajallah, A.; Andreas, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.262 KB) | DOI: 10.14334/jitv.v14i2.354

Abstract

Genotipe of BB kappa kasein (ĸ-kasein) gene positively influences high milk protein yield. The aim of this research was to verify the use of the к-casein gene as marker of assisted selecion (MAS) of high protein yield in domestic Holstein-Friesian (HF) cattle. Genotyping blood samples was conducted for lactating cows from BPPT-SP Cikole (82 head) and a number of small holders in KPSBU Lembang (190 head). Genotyping the к-casein gene was also conducted for active and non active AI bulls (HF) from BIB Lembang (25 head) and BBIB Singosari (32 head). Varian genotipes were identified trough DNA migration, whereas frequencies of alleles were calculated by Nei Method (1987). The effects of variant genotypes on protein yield and other milk component yields were studied in lactating cows (56 head in BPPT-SP Cikole and 111 head in KPSBU Lembang) by applying Least Square Means (LSM) method of the General Linear Model (GLM). Genotyping the ĸ-kasein gene resulted in three genotypes, namely AA, AB and BB, with two alleles, namely A and B. It was identified that a very limited number of lactating cows with BB genotype (0-9%). These cows produced higher protein yield around 3.37-3.84% than that of AA cows, while AB cows produced protein yield in between, though these differences were not statistically significant (P>0,05). In contrast, AA cows tended to produced higher milk fat yield than BB cows. Cows BB and AB seemingly produced higher dry matter, while both specific density and pH were not affected by variant genotypes of the к-casein gene.  The results showed that BB genotype of the к-casein gene tended to be quitely consistent in controlling high protein yield, so it would be a good oppurtinity to be used as MAS of milk protein yield in HF domestic. Key words: Protein Yield, Dairy Cattle (HF), к-Casein Gene, Genotype BB
Polymorphism of growth hormone receptor (GHR) gene in Holstein Friesian dairy cattle Misrianti, Restu; Sumantri, C.; Anggraeni, A.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.675 KB) | DOI: 10.14334/jitv.v16i4.620

Abstract

Growth hormone gene have a critical role in the regulation of lactation, mammary gland development and growth process through its interaction with a specific receptor. Growth hormone (GH) is an anabolic hormone which is synthesized and secreted by somatotrop cell in pituitary anterior lobe, and interacts with a specific receptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested as candidate gene for traits related to milk production in Bovidae. The purpose of this study was to identify genetic polymorphism of the Growth Hormone Receptor (GHR) genes in Holstein Friesian (HF) cattle. Total of 353 blood samples were collected from five populations belonging to Cikole Dairy Cattle Breeding Station (BPPT-SP Cikole) (88 samples), Pasir Kemis (95 samples), Cilumber (98 samples), Cipelang Livestock Embryo Center (BET Cipelang) (40 samples), Singosari National Artificial Insemination Centre (BBIB Singosari) (32 samples) and 17 frozen semen samples from Lembang Artificial Insemination Center (BIB Lembang). Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques then PCR products were genotyped by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. There were two allele dan three genotypes were found namely: allele A and G, Genotype AA, AG and GG repectively. Allele A frequency (0.70-0.82) relatively higher than allele G frequency (0.18-0.30). Chi square test show that on group of BET Cipelang, BIB Lembang and BBIB Singosari population were not significantly different (0.00-0.93), while on group of BET Cipelang, BIB Lembang dan BBIB Singosari population were significantly different (6.02-11.13). Degree of observed heterozygosity (Ho) ranged from 0.13-0.42 and expected heterozygosity (He) ranged from 0.29-0.42. Key Words: Growth Hormone Receptor, Polymorphism, Holstein Friesian Cattle
IDENTIFICATION OF GH|ALUI AND GHR|ALUI GENES POLYMORPHISMS IN INDONESIAN BUFFALO Andreas, E.; Sumantri, C.; Nuraini, H.; Farajallah, A.; Anggraeni, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 4 (2010): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.4.215-221

Abstract

Growth hormone (GH) is an anabolic hormone which sintesized and secreted by somatrotop cell inpituitary anterior lobe. GH exert its effect on growth and metabolism by interacting with a specificreceptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested ascandidate gene for traits related to meat production in Bovidae. The objectives of this study were toidentify polymorphism of GH and GHR genes in buffalo. The 452 DNA samples buffalo were collectedfrom five populations in Indonesia (Siborong-Borong-Medan (65), Lebak-Banten (29), Pandeglang-Banten (180), Semarang-Central Java, and Mataram-West Nusa Tenggara (103)). A gene fragment of theGH|AluI gene at 432 bp located on exon 3 and GHR|AluI gene at 298 bp on exon 10 were successfullyamplified by using the techniques of a PCR (polymerase chain reaction) and genotyped by PCR-RFLP(restriction fragment length polymorphism) then -SSCP (single strand conformation polymorphism). Theresults showed no polymorphisms were detected in these genes. All buffaloes tested had LL genotype forlocus GH|AluI and AA genotype for locus GHR|AluI.
Association of Liver X Receptor Alpha (LXRα) gene related to characteristic of carcass, meat quality and fatty acid composition in ducks Gunawan, A.; Azmi, N.; Anggraeni, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 2 (2021): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.2.114-123

Abstract

Liver X Receptor Alpha (LXRα) is a nuclear receptor that play a crucial role in regulating of gene involvedin lipid metabolism. The aim of this research was to identify polymorphisms and association of LXRα gene with charateristic of carcass, meat quality and fatty acid composition in ducks using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). A total sample of 98 Cihateup ducks consisted of 57 females and 41 males with age 12 weeks were used in this study. Product size is 661 bp amplicons. The genotype genes frequencies in CC, GC and GG were 0.21, 0.55 and 0.23 respectively. The chi-square test revealed that LXRα gene (g.3575 C>G) in exon 2 was in Hardy-Weinberg equilibrium. A SNP of LXRα gene in region g.3575 C>G was significantly associated (P<0.05) with duck meat quality and fatty acid content. Several parameters have significant affect (P <0. 05) on meat quality in the breast meat weight, carcass percentage and head percentage, while associated fatty acids were saturated fatty acids (SFA) such as palimitic acid (C16:0); γ-and unsaturated fatty acids (UFA) such as linolenic acid (C18:3n3); cis 11 eicosenoic acid (C20:1) and 11.14 cis-eicosedenoic acid (C20:2). In could be concluded that LXRα gene might be useful as genetic markers to select and produce meat with desirable unsaturated fatty acids.
Co-Authors A. GUNAWAN ACHMAD FARAJALLAH C. SUMANTRI E. Andreas H. Nuraini N. Azmi, N. Restu Misrianti