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Polymorphism of growth hormone receptor (GHR) gene in Holstein Friesian dairy cattle Misrianti, Restu; Sumantri, C.; Anggraeni, A.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Growth hormone gene have a critical role in the regulation of lactation, mammary gland development and growth process through its interaction with a specific receptor. Growth hormone (GH) is an anabolic hormone which is synthesized and secreted by somatotrop cell in pituitary anterior lobe, and interacts with a specific receptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested as candidate gene for traits related to milk production in Bovidae. The purpose of this study was to identify genetic polymorphism of the Growth Hormone Receptor (GHR) genes in Holstein Friesian (HF) cattle. Total of 353 blood samples were collected from five populations belonging to Cikole Dairy Cattle Breeding Station (BPPT-SP Cikole) (88 samples), Pasir Kemis (95 samples), Cilumber (98 samples), Cipelang Livestock Embryo Center (BET Cipelang) (40 samples), Singosari National Artificial Insemination Centre (BBIB Singosari) (32 samples) and 17 frozen semen samples from Lembang Artificial Insemination Center (BIB Lembang). Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques then PCR products were genotyped by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. There were two allele dan three genotypes were found namely: allele A and G, Genotype AA, AG and GG repectively. Allele A frequency (0.70-0.82) relatively higher than allele G frequency (0.18-0.30). Chi square test show that on group of BET Cipelang, BIB Lembang and BBIB Singosari population were not significantly different (0.00-0.93), while on group of BET Cipelang, BIB Lembang dan BBIB Singosari population were significantly different (6.02-11.13). Degree of observed heterozygosity (Ho) ranged from 0.13-0.42 and expected heterozygosity (He) ranged from 0.29-0.42. Key Words: Growth Hormone Receptor, Polymorphism, Holstein Friesian Cattle

KANDUNGAN FRAKSI SERAT TEPUNG SILASE AMPAS TEBU YANG DITAMBAH BIOMASA INDIGOFERA SEBAGAI PAKAN TERNAK Arsyadi Ali; Bambang Kuntoro; Restu Misrianti
Jurnal Peternakan Vol 16, No 1 (2019): Februari 2019
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v16i1.6213

Ampas tebu merupakan Iimbah pabrik gula yang banyak ditemukan dan dapat mencemari lingkungan apabila tidak dimanfaatkan. Saat ini belum banyak peternak menggunakan ampas tebu tersebut untuk bahan pakan ternak. Tujuan dari penelitian ini adalah untuk mengetahui mengetahui pengaruh proses ensilase dan penambahan biomas Indigofera terhadap fraksi serat tepung ampas tebu. Penelitian dilakukan secara eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL). Berdasarkan hasil penelitian ini dapat ditarik kesimpulan bahwa penambahan biomasa Indigofera belum mampu menurunkan kandungan neutral detergent fibre (NDF), acid detergent fibre (ADF), dan Acid detergent lignin (ADL) tepung silase ampas tebu.

KERAGAMAN SIFAT KUALITATIF DAN KUANTITATIF SAPI KUANTAN PADA BERBAGAI TINGKATAN UMUR DIKECAMATAN BENAI Restu Misrianti; Rona Perti Mustika; Arsyadi Ali
Jurnal Peternakan Vol 15, No 2 (2018): September 2018
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v15i2.4078

This research aims to know the diversity of the nature of qualitative and quantitative kuantan cows age on many levels in Benai District of Kuantan Singingi regency. The number of samples was observed 88 cows kuantan which consists of 71 cows female and 17 male cows with levels 0-6 months, 6-12 months, 12-18 months and 18-24 months. The parameters observed in the qualitative nature of hair and skin color is, horns shape and color away, while the nature of quantitative measurements of the body namely chest circumference, length, shoulder height, in the chest and hip height. The results showed that the qualitative nature of the cows kuantan females include the dominant hair color that is the color of tanned 35,21% white, curved Horn shape up and short horns and small alike 29.5% and the dominant leg color is 68% white and male cow whereas in kuantan, the dominant hair color that is the color of tanned 35,30% white, horned 53% not horned and short small 29% , and the dominant leg color is white 76%. Quantitative trait cow kuantan females include the dominant length is age 18-24 months 96,28 ± 10.70 cm, chest circumference: 20,71 ± 12.52 cm, in the chest: 43,28 ± 0.14 cm, shoulder height: 96,57 ± 7.25 cm and a height of hips: 101,71 ± 9.94 cm and while the average body size of cow kuantan males include the dominant length is aged 6-12 months 87 ± 6.05 cm, chest circumference of 6-12 months ± 16,52 112,75 cm chest, in 12-18 months 41,33 ± 1.53 cm shoulder height, age 18-24 months orders of 4.16 ± 91,67 cm high hip and age 12-18 months of 6.08 ± 101 cm.

Polymorphism of growth hormone receptor (GHR) gene in Holstein Friesian dairy cattle Restu Misrianti; C. Sumantri; A. Anggraeni
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 4 (2011): DECEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Growth hormone gene have a critical role in the regulation of lactation, mammary gland development and growth process through its interaction with a specific receptor. Growth hormone (GH) is an anabolic hormone which is synthesized and secreted by somatotrop cell in pituitary anterior lobe, and interacts with a specific receptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested as candidate gene for traits related to milk production in Bovidae. The purpose of this study was to identify genetic polymorphism of the Growth Hormone Receptor (GHR) genes in Holstein Friesian (HF) cattle. Total of 353 blood samples were collected from five populations belonging to Cikole Dairy Cattle Breeding Station (BPPT-SP Cikole) (88 samples), Pasir Kemis (95 samples), Cilumber (98 samples), Cipelang Livestock Embryo Center (BET Cipelang) (40 samples), Singosari National Artificial Insemination Centre (BBIB Singosari) (32 samples) and 17 frozen semen samples from Lembang Artificial Insemination Center (BIB Lembang). Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques then PCR products were genotyped by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. There were two allele dan three genotypes were found namely: allele A and G, Genotype AA, AG and GG repectively. Allele A frequency (0.70-0.82) relatively higher than allele G frequency (0.18-0.30). Chi square test show that on group of BET Cipelang, BIB Lembang and BBIB Singosari population were not significantly different (0.00-0.93), while on group of BET Cipelang, BIB Lembang dan BBIB Singosari population were significantly different (6.02-11.13). Degree of observed heterozygosity (Ho) ranged from 0.13-0.42 and expected heterozygosity (He) ranged from 0.29-0.42. Key Words: Growth Hormone Receptor, Polymorphism, Holstein Friesian Cattle

Determination of Morphological Characteristics in Kuantan Cattle using Multivariate Analysis Restu Misrianti; Jessy Mainidar; Hasrul Bani Asharudin; Yureni Sahril Dedi; Arsyadi Ali; Sony Hartono Wijaya; Cece Sumantri; Jakaria Jakaria
Buletin Peternakan Vol 45, No 3 (2021): BULETIN PETERNAKAN VOL. 45 (3) AUGUST 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i3.66868

The objective of this research was to characterized morphology and estimated genetic distance between intra population of Kuantan cattle. A Total of 213 cattle (44 male and 169 female with age ranging from 2-3 years) were used in this study and collected from extensive ranging systems in Three sub-population (Cerenti, Inuman, and Kuantan Hilir regions) Kuantan Singingi Regency, Riau Province. Five variables were measured that is Body Length (BL)(cm), Wither Height (WH)(cm), Hip Height (HH)(cm), Chest Girth (CG)(cm), and Chest Depth (CD)(cm). Data obtained were descriptive analysis, Principal Components Analysis (PCA) and Hierarchichal Clustering Analysis (HCA) using XLSTAT program. All variables of body measurement in the Kuantan Hilir region were higher than Cerenti dan Inuman, Kuantan Singingi Regency. The first factor in PCA described body measurement contributed 32.77%, and the second factor described body shape contribute 25.83% of total variability. The dendrogram showed there is Three clusters of Kuantan Cattle based on this research.

Pemanfaatan Tepung Biji Alpukat (Persea americana Mill.) dalam Ransum terhadap Performa Ayam Ras Pedaging Khalida M. N. Harahap; Edi Erwan; Restu Misrianti
Jurnal Peternakan Sriwijaya Vol 8, No 2 (2019): JURNAL PETERNAKAN SRIWIJAYA
Publisher : Department of Animal Sciences, Faculty of Agriculture, Sriwijaya University, Indonesia

Biji alpukat (Persea americana Mill.) merupakan limbah yang mengandung nutrisi diantaranya protein kasar, energi metabolis yang dijadikan digunakan sebagai bahan pakan unggas. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan tepung biji alpukat (TBA) di dalam ransum terhadap performa ayam ras pedaging yang meliputi konsumsi ransum, pertambahan bobot badan, konversi ransum dan income over feed cost. Penelitian ini telah dilaksanakan pada bulan Maret sampai April 2019 di laboratorium UIN Agriculture Research and Development Station (UARDS) Fakultas Pertanian dan Peternakan UIN Suska Riau. Penelitian ini menggunakan 80 ekor DOC strain cobb yang diberi ransum basal dengan penambahan TBA yang dipelihara selama 5 minggu. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 5 ulangan. Perlakuan yang diberikan terdiri dari T0 (ransum basal dengan TBA 0%), T1 (ransum basal dengan TBA 5%), T2 (ransum basal dengan TBA 10%), dan T3 (ransum basal dengan TBA 15%). Hasil penelitian ini menunjukkan bahwa penggunaan tepung biji alpukat dalam ransum sampai 15% tidak berpengaruh nyata (P>0,05) terhadap konsumsi ransum namun berpengaruh sangat nyata (P<0,01) menurunkan pertambahan bobot badan, konversi ransum dan income over feed cost ayam ras pedaging. Kesimpulan dari penelitian ini adalah tepung biji alpukat dapat digunakan hingga 5% dalam ransum ayam ras pedaging.

IDENTIFIKASI UKURAN TUBUH DAN KUALITAS SEMEN SAPI KUANTAN JANTAN Yendraliza Yendraliza; Husnul Abadi; Restu Misrianti; Arsyadi Ali; Anwar Effendi
JURNAL ILMIAH PETERNAKAN TERPADU Vol 7, No 1 (2019)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Kuantan cattle is one of the Indonesia's local cattle originating from Riau Province. This research were aimed to determine the average body size and test the fresh semen quality of Kuantan cattle. Measurements of Kuantan cattle body were carried out in 16 adult bulls by observing body length, chest circumference, and shoulder height. Semen quality testing was carried out on four years old bulls with ±150kg weight with the parameters are odor, color, pH, volume, consistency and degrees of viscosity, motility, viability and abnormality. The results showed that the average body size of cattle in Kuantan included body length (cm), chest circumference (cm) and shoulder height (cm) of Kuantan cattle were 88.45 ± 3.92, 119.77 ± 16.18 and 98.94 ± 10.28, while motility, viability, abnormalities were 75.00%, 81.33%, 6.8%. The conclusions of this study were Kuantan cattle body size in small size and the semen feasible to be diluted into frozen semen. Keywords: Body Size, Semen Quality, Kuantan Cattle

Keragaman Gen Myxovirus (Mx) pada Itik di Kabupaten Indragiri Hulu Provinsi Riau Restu Misrianti; Elfawati Elfawati; Apri Irianto
Musamus Journal of Livestock Science Vol 5 No 1 (2022): Musamus Journal of Livestock Science
Publisher : Faculty of Agriculture, Musamus University

Show Abstract | Download Original | Original Source | Check in Google Scholar

The Mx gene (Myxovirus) is a gene that functions to control the immunity of poultry against viral diseases. This gene is a native antiviral and is able to respond to influenza virus infections such as avian influenza. This study aims to identify the diversity of the Mx gene in ducks using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) molecular technique. The number of samples used were thirteen ducks from Indragiri Hulu Regency (Riau Province). The restriction site of the Mx gene using the Rsa1 enzyme is in the base sequence GTN|NAC. There are two alleles namely A and G, and three genotypes, AA, AG and GG. The results showed that the Mx gene in ducks was monomorphic. The frequency of the resistant allele (A) is 0%, while the frequency of the susceptible allele (G) is 100%.

LIVABILITY AND RECOVERY RATE OF BALI CATTLE SPERMATOZOA DURING PRESERVATION IN TRIS-BASED EGG YOLK DILUENT WITH DIFFERENT SUCROSE LEVELS Yendraliza Yendraliza; Tania Tania; Restu Misrianti; Zumarni Zumarni
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

This study aimed to determine the effect of sucrose addition in tris-based egg yolk diluent in maintaining and protecting spermatozoa during preservation. The design of this study was a completely randomized design (CRD) which consisted of five treatments with ten replications. The treatments were tris-based egg yolk diluent without sucrose (P0, control), tris-based egg yolk diluent with 0.2% sucrose (P1), tris-based egg yolk diluent with 0.3% sucrose (P2), tris-based egg yolk diluent with 0.4% sucrose (P3) and tris-based egg yolk diluent with 0.5% sucrose (P4). The parameters measured were motility, livability, abnormality, intact plasma membrane, and recovery rate. Semen was evaluated 2 times, freshly after being collected and post thawed. The results showed that the increase level of sucrose addition from 0.2% to 0.5% increased the motility value from 43.59% to 48.15%, the livability value from 51.24% to 55.45%, the intact plasma membrane value from 44.66% to 48.21%, the recovery rate value from 54.6% to 60.2 %, and reduce the abnormality value from 13.49%-10.24%. It can be concluded that the addition of 0.2- 0.5% sucrose in tris-based egg yolk diluent could increase motility, livability, intact plasma membrane, recovery rate, and could reduce the abnormalities of Bali cattle spermatozoa during preservation

Profil Nutrisi dan Fraksi Serat Pakan Silase Komplit Berbahan Ampas Tebu dengan Penambahan Legume Indigofera dan Molases Muhamad Rodiallah; Anwar Efendi Harahap; Arsyadi Ali; Triani Adelina; Dewi Ananda Mucra; Bakhendri Solfan; Restu Misrianti; Jepri Juliantoni; Evi Irawati; Bayu Nuari Ramadhan
JURNAL TRITON Vol 14 No 1 (2023): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v14i1.377

Ampas tebu memiliki komponen serat yang tinggi sehingga perlu adanya perbaikan nutrisi dengan penambahan legume indigofera yang diolah menggunakan teknik silase. Tujuan penelitian ini untuk menganalisis profil nutrisi dan fraksi serat ampas tebu yang disilase dengan penambahan legume indigofera dan molases. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) Pola Faktorial (3x2) dengan 3 ulangan. Faktor A terdiri dari: A1 = 100 % ampas tebu; A2 = 75 % ampas tebu + 25 % indigofera; A3 = 50 % ampas tebu + 50 % indigofera, selanjutnya faktor B terdiri dari: B0 = 5 % molases; B1 = 10 % molases. Parameter yang diamati meliputi kandungan nutrient silase (bahan kering, protein kasar, lemak kasar, serat kasar, abu dan Bahan Ekstrak Tanpa Nitrogen) % serta fraksi serat meliput (NDF dan ADF) %. Hasil penelitian ini menunjukkan tidak terdapat interaksi (P>0.05) antara proporsi bahan ampas tebu + legume indigofera dengan penambahan molases terhadap parameter bahan kering, protein kasar, lemak kasar, dan Bahan Ekstrak Tanpa Nitrogen, serat kasar, NDF, dan ADF) %, tetapi faktor perlakuan proporsi bahan ampas tebu + legume indigofera berpengaruh nyata (P<0,05) terhadap bahan kering, protein kasar, abu, serat kasar, NDF dan ADF . Proporsi silase berbahan 50 % ampas tebu + 50 % legume indigofera menghasilkan nilai protein kasar tertinggi serta serat kasar, NDF, dan ADF terendah dibandingkan dengan kombinasi perlakuan lainnya. Selanjutnya penambahan molases hingga 10 % pada proses silase belum memperlihatkan kemampuan memperbaiki kualitas nutrisi dan fraksi serat secara keseluruhan.

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