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All Journal Jurnal Kedokteran Diponegoro Majalah Kedokteran Gigi Indonesia
RR Mahayu Dewi Ariani
Department Of Medical Biology And Biochemistry, Faculty Of Medicine, Diponegoro University, Tembalang, Semarang, Indonesia 50275
Dentistry Medicine & Pharmacology

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THE EFFECT OF GIVING BIT JUICE (Beta vulgaris L.) ON SPERMATOZOA MORPHOLOGY OF WISTAR RATS THAT EXPOSED MOSQUITO COIL SMOKE Septian, Kevin; Ariani, RR Mahayu Dewi; Hermawati, Donna; Nugroho, Trilaksana
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 2 (2020): DIPONEGORO MEDICAL JOURNAL ( Jurnal Kedokteran Diponegoro )
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.542 KB) | DOI: 10.14710/dmj.v9i2.27145

Abstract

Background : Spermatozoa have a limited amount of antioxidants that cause spermatozoa to be susceptible to oxidative stress by reactive oxygen species (ROS). Mosquito repellent contains the active ingredient Pyrethroid which is one example of external factors that cause DNA damage through oxidative stress. This results in the emergence of secondary metabolites that can act as free radicals, then these free radicals follow the circulation of blood throughout the body including the testes which will cause the process of spermatogenesis to be disrupted and will ultimately affect the quality of the spermatozoa produced. Objective :  To find out the effect of giving beetroot juice (Beta vulgaris) on the morphology of spermatozoa of male Wistar rats that exposed to mosquito coil smoke. Method : This study uses an experimental study, with the type of research design is post test only control group design. The population used in this study were male Wistar rats (Rattus novergicus). The research sample was selected by simple random sampling. Analysis of the data used in the study is an analysis with one way anova test. Results : The mean morphological morphology of normal spermatozoa are: Group K (-) = 96.40; Group K (+) = 88,40; P1 group = 90.80; P2 group = 94.40. One Way ANOVA test found significant differences between the five treatment groups. Post-Hoc Test found significant differences between K (-) with K (+), K (+) with P2, P1 with P2, P1 with K (-). While in the other groups, there was no significant difference in the morphological percentage of spermatozoa. Conclusion : The giving of beet juice can affect sperm morphology Wistar rats that exposed to mosquito coil smoke.Keywords : spermatozoa, bit juice, antioxidant, free radical
Effect of temperature and passive ultrasonic irrigation of EDTA 17% in smear layer removal Fitra Nabiila Alfiona; RR Mahayu Dewi Ariani; Nadia Hardini; Gloria Fortuna
Majalah Kedokteran Gigi Indonesia Vol 7, No 1 (2021): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.64026

Abstract

Smear layer removal in root canal treatment is required to aid the diffusion of intracanal medicament and help sealer penetrate into dentinal tubules. The need for irrigants is important to facilitate smear layer removal in the root canal. Various methods have been proposed in some articles, but their application in apical third still challenges many clinicians due to the complexity of the root canal. This study aimed to analyze the effect of temperature and passive ultrasonic irrigation (PUI) activation of EDTA 17% solution in smear layer removal. Twenty-four extracted mandibular premolars were decoronated to standard root length of 14 mm. The preparation of root canals was completed with crown-down technique using ProTaper FHU to file F3 (30/.09). The root canal irrigation was done with NaOCl 5.25% and EDTA 17% solution. The samples were divided into four groups, namely 25 ºC of EDTA 17% solution (group 1), 37 ºC of EDTA 17% solution (group 2), 25 ºC of EDTA 17% solution with PUI activation (group 3), and 37 ºC of EDTA17% solution with PUI activation (group 4). Smear layer removal was observed under scanning electron microscopy (SEM) and scored ordinally from 1-5. Non-parametric Friedman test showed significant results in all the groups (p<0.05). Post hoc Wilcoxon Signed Rank test showed significant results between group 2 and group 3 (p = 0.039), and between group 3 and group 4 (p = 0.038). The combination methods of temperature and PUI activation showed a significant result in smear layer removal.
LIQUID SMOKE SUBMERSIVE EFFECTIVENESS OF ACRYLIC PLATE TO THE GROWTH OF CANDIDA ALBICANS Mochamad Haldi Tri Hutama; Windriyatna Windriyatna; Dwi Ngestiningsih; RR Mahayu Dewi Ariani; Tyas Prihatiningsih
JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL) Vol 10, No 5 (2021): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v10i5.30115

Abstract

Background: Acrylic denture was an alternative to replace a lost tooth, and surrounding tissues have the part of the acrylic plate, which was frequently a place of Candida albicans proliferation. The liquid smoke has a chemical compound of phenol which produced antimicrobial and could hamper or destroy Candida albicans.Objectives: To identify the acrylic plate's effectiveness, soaking into liquid smoke to the growth of Candida albicans.Methods: This research was categorized into experimental research which exerted post-test control group research design. The total sample in this research was 25 samples, four treatment groups, and one control group. This treatment covered acrylic plate soaking into liquid smoke in specific concentration level 1%, 2%, 4%, and 6% to obstruct the growth of Candida albicans. Moreover, the statistic test was conducted through Kruskal-Wallis and continued to Post-Hoc test through Mann-Whitney theory.Findings: This research showed a significant difference between the amount of Candida albicans on the control group and liquid smoke group in concentrations 1%, 2%, 4%, and p-valuable = 0,005). Further, based on the Post-Hoc test result of Mann-Whitney, it referred significant difference between concentrations 1%, 2%, 4%, and 6% within sterile aquades (p-value = 0,008).Conclusions: The liquid smoke shared effective concentration which functioned to hamper Candida albicans on the acrylic plate. The concentration level of 6% was the most effective concentration to interfere Candida albicans on the acrylic plate. Furthermore, it was not found any significant difference between resistibility of liquid smoke to Candida albicans on an acrylic plate with concentration level of 1%-6% which could be the most effective way to obstruct Candida albicans on acrylic plate.Keywords : Acrylic plate, Liquid smoke, Candida albicans 
Co-Authors Djuara P Lubis Donna Hermawati Fitra Nabiila Alfiona Gloria Fortuna Mochamad Haldi Tri Hutama Nadia Hardini Nugroho, Trilaksana Prihatiningsih, Tyas Septian, Kevin Windriyatna Windriyatna