Various microorganisms such as Streptococcus pneumonia, Haemophilus influenza, Chlamydia spp, and Mycoplasma pneumonia can cause ARI. The ginger flower which contains various phytochemicals that have potential anti-microbial. Recent study was aimed to explore the anti-microbial properties of ginger flower extract. This study was experimental in vitro study with the disc diffusion method. The ethanol extract of Ginger flowers was obtained by maceration method with a ratio of Simplicia to solvent 1:10. Then it concentrated with a rotary evaporator. The ethanol extract of the ginger flower was diluted by DMSO into four concentrations viz. 100%, 75%, 50%, and 25%. The anti-microbial activity assay used three different types of microbes (Streptococcus pneumonia, Haemophilus influenza, and Candida albicans) different in 3 repetitions. There were significant differences each concentration on the width of clear zone among Streptococcus pneumonia (P-value = 0.010), Haemophilus influenza (P-value = 0.011), and Candida albicans (P-value = 0.005). Also, all concentrations of ginger flower extract showed no significant difference between positive controls; however, significant differences were found for negative controls. Hence, It can be concluded that 25% of ginger flower extract has been sufficient to inhibit the growth of various tested microorganisms.