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All Journal Jurnal Kesehatan Masyarakat Jurnal Ilmu Ternak dan Veteriner Universa Medicina
wayan T Artama
Veterinary Medicine Faculty, UGM Yogyakarta
Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Sequence variability of the β Tubulin isotype-1 genes in benzimidazole resistant strains of Haemonchus contortus, a nematode parasite of sheep Haryuningtyas, Dyah; Artama, wayan T; Asmara, Widya
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.925 KB) | DOI: 10.14334/jitv.v11i3.531

Abstract

Resistance to benzimidazole at a population of gastrointestinal nematode, especially Haemonchus contortus is a widespread problem. Therefore, anthelmintic will not effective anymore. Benzimidazole resistance in nematode has been shown to be heritable and defined as an increase in frequency of individual worms able to drug tolerate. In Indonesia resistance to anthelmintic albendazole has been reported on sheep in district of Bogor, Kuningan, Kendal and province of Yogyakarta. Molecular mechanism of resistance to benzimidazole on nematode and fungi related to single gene which code ß tubulin. The aim of this research is to determine a genetics variability of isotype-1 ß tubulin genes from Indonesian strain of H. contortus which resistant to benzimidazole. Sequencing were carried out at a 520 bp fragment of the isotipe-1 ß tubulin gene of six H. contortus resistant strain from four sheep from SPTD Trijaya farm, district of Kuningan, West Java; UPTD Pelayanan Kesehatan Hewan farm, district of Bantul, Yogyakarta respectively. One H. contortus susceptible strains as a control come from farmer from Cicurug, district of Sukabumi, West Java. Results indicated that there are genetics variability of  fragment isotype-1 ß tubulin genes from H. contortus from UPTD Pelayanan Kesehatan Hewan farm, Bantul, Yogyakarta (H1, H2, and C2). There are no genetics variability in the fragment gene of H. contortus resistant strain from SPTD Trijaya farm, Kuningan, West Java (Kn1, Kn2 and K1). Genetic variability occur between 2 isolate from different location. Key Words: Haemonchus contortus, Benzimidazole, Isotype 1 β-tubulin Gene, Sequencing
Effectiveness of Environmentally Friendly Mosquito Trap Contained Sugar Yeast Solution Rosanti, Tutik Ida; Mardihusodo, S Juwono; Artama, Wayan T
Jurnal Kesehatan Masyarakat Vol 12, No 2 (2017): Jurnal KEMAS Vol.12 No.2 : January 2017
Publisher : Jurusan Ilmu Kesehatan Masyarakat Fakultas Ilmu Keolahragaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v12i2.6063

Abstract

Environmentally friendly mosquitoes trap using common daily materials used by community may give hopes in reducing mosquitoes density. This study aims to determine the effectiveness of environmentally friendly mosquitoes trap made from bottle contained sugar yeast solution for reducing the number of trapped mosquitoes. This study consist of two phases and quasi experimental design was used. Mann Whitney test was used to determine the differences the number of trapped mosquitoes indoor and outdoor. The result showed p value 0,000 which was lower than α value (0,05), so there was significant difference between the number of indoor and outdoor trapped mosquitoes. The average Rank score of outdoor mosquitoes trap (42,75) was more than indoor mosquitoes trap (18,25). We concluded that the mosquitoes trapped which contained of sugar-yeast solution was effective for trapping the mosquitoes especially outdoor .
RET single nucleotide polymorphism in Indonesians with sporadic Hirschsprung’s disease Saryono, Saryono; Rochadi, Rochadi; Lestariana, Wiryatun; Artama, Wayan T; Sadewa, Ahmad Hamim
Universa Medicina Vol 29, No 2 (2010)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2010.v29.71-77

Abstract

The tyrosine kinase receptor RET, which is the protein product of the RET gene, is involved in the development of the mammalian nervous system that causes Hirschsprung’s disease (HSCR). RETs are cell surface molecules that are expressed in cells derived from the neural crest. The purpose of this study was to investigate the polymorphism of the RET gene in HSCR in the Yogyakarta population. Genomic DNA was extracted from surgically removed bowel tissues of 54 unrelated HSCR patients. Exon 2 of the RET gene was amplified by polymerase chain reaction (PCR) and analyzed by restriction fragment length polymorphism (RFLP). Molecular results were compared with clinical performance of Hirschsprung patients. RET polymorphism was detected in exon 2 in all of the 54 Indonesian HSCR patients. The allelic distribution of the c135GàA polymorphism in the RET exon 2 indicated that the A allele was more frequent in patients than in control individuals (chi-square test, p= 0.001). Thus the RET variant allele A is over-represented in patients affected with the HSCR phenotype. Polymorphism of exon 2 of the RET gene was found in sporadic Hirschsprung’s disease in the Yogyakarta population, which suggests that the RET gene plays important roles in the pathogenesis of HSCR.
Effectiveness of Environmentally Friendly Mosquito Trap Contained Sugar Yeast Solution Rosanti, Tutik Ida; Mardihusodo, S Juwono; Artama, Wayan T
KEMAS: Jurnal Kesehatan Masyarakat Vol 12, No 2 (2017)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v12i2.6063

Abstract

Environmentally friendly mosquitoes trap using common daily materials used by community may give hopes in reducing mosquitoes density. This study aims to determine the effectiveness of environmentally friendly mosquitoes trap made from bottle contained sugar yeast solution for reducing the number of trapped mosquitoes. This study consist of two phases and quasi experimental design was used. Mann Whitney test was used to determine the differences the number of trapped mosquitoes indoor and outdoor. The result showed p value 0,000 which was lower than α value (0,05), so there was significant difference between the number of indoor and outdoor trapped mosquitoes. The average Rank score of outdoor mosquitoes trap (42,75) was more than indoor mosquitoes trap (18,25). We concluded that the mosquitoes trapped which contained of sugar-yeast solution was effective for trapping the mosquitoes especially outdoor .
Co-Authors Ahmad Hamim Sadewa Dyah Haryuningtyas Rochadi Rochadi, Rochadi S Juwono Mardihusodo, S Juwono Saryono Saryono Tutik Ida Rosanti Widya Asmara Wiryatun Lestariana, Wiryatun