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Journal : Tropical Health and Medical Research Journal

Number of Breast Milk Bacteria in Women with Normal Labor and Caesarean Delivery During Room Temperature Storage Habibah, Siti Noor; Muhlisin, Ahmad; Lutpiatina, Leka; Insana, Aima
Tropical Health and Medical Research Vol. 6 No. 1 (2024): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v6i1.111

Abstract

The presence of microbes in each breast milk sample can be different, one of which is influenced by the method of delivery. The research aimed to analyze the effect of storage time for breast milk from mothers giving birth normally and giving birth by Caesarean on the number of bacteria at room temperature. The independent variable in this study was the storage time for breast milk from mothers giving birth normally and giving birth by Caesarean for 0, 4, 8, and 12 hours at room temperature, while the dependent variable in this study was the number of bacteria in the breast milk. The research samples were breast milk from mothers who gave birth normally and Caesarean section which was treated with storage at room temperature for 0, 4, 8, and 12 hours. The results of the research were that the average number of bacteria in breast milk from mothers giving birth normally, stored at 0, 4, 6, 8, and 12 hours respectively, was 1.33 x 104 CFU/ml; 2.18 x 104 CFU/ml; 5.04 x 106 CFU/ml; 1.68 x 107 CFU/ml. The average number of bacteria in breast milk from mothers giving birth by Caesarean storage 0, 4, 6, 8, and 12 hours respectively 1.83 x 104 CFU/ml; 1.73 x 105 CFU/ml; 1.76 x 107 CFU/ml; 6.67 x 107 CFU/ml. There is a significant difference in the number of bacteria between breast milk from mothers who gave birth normally and breast milk from mothers who gave birth by Caesarean section. Storage time has a positive effect on the number of bacteria in breast milk from mothers giving birth normally and giving birth by Caesarean. It is recommended to store breast milk at room temperature for up to 4 hours for breast milk from women giving birth normally and for breast milk from mothers giving birth by Caesarean, storage for less than 4 hours by paying attention to the cleanliness of the breast milk storage area.
Differences in Thawing Methods in Broiler Chicken Meat on Total Plate Count (TPC) of Bacteria Putri, Noorlaily; Lutpiatina, Leka; Nurlailah; Insana, Aima
Tropical Health and Medical Research Vol. 6 No. 2 (2024): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v6i2.120

Abstract

Total Plate Count (TPC) determines the number of microorganisms, both bacteria and fungi. Thawing is a step in thawing food that was previously stored frozen. This study aims to analyze the differences in the Total Plate Count of bacteria with variations in thawing methods in broiler chicken meat. Thawing methods commonly used include thawing at room temperature, soaking in water, and leaving it in the refrigerator (chiller). The type of research is experimental design with a posttest-only control design. Repetition was carried out five times for each control group KN (negative control) and test groups R0 (fresh chicken), R1 (frozen chicken), R2 (chicken thawed at room temperature), R3 (chicken soaked in water) and R4 (chicken thawed in the chiller) then the number of bacteria that grew was calculated. The average number of R0 colony results ranged from 4.1 x 103 CFU/g, R1 8.6 x 102 CFU/g, R2 1.6 x 104 CFU/g, R3 8.9 x 103 CFU/g and R4 1.2 x 103 CFU/g. The Kruskall-Wallis test results showed an Asymp sig value of 0.000 (<0.05); it can be concluded that there are differences in TPC in broiler chicken meat with variations in thawing methods that are thawed at room temperature, soaked in water and thawing in the chiller. It is recommended to thaw frozen chicken meat using the method of leaving it in the refrigerator because it has the lowest number of germs and can inhibit the growth of germs.