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All Journal Jurnal Teknologi Laboratorium Medical Laboratory Technology Journal Meditory : The Journal of Medical Laboratory Jurnal Skala Kesehatan JCPS (Journal of Current Pharmaceutical Sciences) Jurnal Riset Kefarmasian Indonesia Indian Journal of Forensic Medicine & Toxicology JPEMAS : Jurnal Pengabdian Kepada Masyarakat

leka lutpiatina, leka

Poltekkes Kemenkes Banjarmasin

Author-ID : 616250

Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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CEMARAN Staphylococcus aureus DAN Pseudomonas aerogenosa PADA STETESKOP DIRUMAH SAKIT lutpiatina, leka
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Medical devices that are susceptible to bacteria contaminated one of them is a stethoscope. Nosocomial infections that are problematic in the hospital include Staphylococcus aureus and Pseudomonas aerogenosa. These bacteria can cause mild skin infections to serious life-threatening infections. The purpose of this research is to know the existence of bacteria contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop at Banjarbaru hospital. This research uses descriptive survey method, which is a research method to describe objectively based on facts found. The samples were stethoscope in child care room, internal medicine room, delivery room, post partum room, perinatology room, and post-surgical treatment room at Banjarbaru hospital for 30 stethoscopes.The result of the research was found contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop. The site home condition of the research data was 66.7% cleaned daily, the storage method was placed on the table 70% and the duration of using the set home more than 1 year as much as 70%. The conclusion of stethoscope at Banjarbaru Hospital was contaminated with Staphylococcus aureus by 70% and Pseudomonas aerogenosa by 17%. The suggestion of research can be continued by knowing the existence of Staphylococcus aureus resistant antibiotic and Pseudomonas aerogenous antibiotic resistant at steteskop at Hospital.

Clove (Syzygium aromaticum) Effect on Growth Malassezia Furfur and Aspergillus sp on Media Leka Lutpiatina1 , Pebri Fitria Lestari2 , Dwi Intan Purnama Sari2 , Haitami1 , Rifqoh1 , Nurlailah1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10539

Background: Malassezia furfur and Aspergillus sp are fungi associated with infection in humans. One of the plants that can use as anti fungi is clove (Syzygium aromaticum). Aim: The purpose of this study was to determine the growth barriers of Malassezia furfur and Aspergillus sp on media with the addition of Clove Powder (Syzygium aromaticum). Material and Method: The equipment used by cloves (Syzygium aromaticum) is a part of the reddish green flower. The samples used in this study were Malassezia furfur and Aspergillus sp. Specimens planted on Saboraud Dextrose Agar media with the addition of clove powder (Syzygium aromaticum) in various concentrations. Results: Malassezia furfur in media with clove powder concentration of 0%; 0.05%; 0.1% shows the number of colonies 1330; 1135; 765. Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus on media with clove powder concentration of 0%; 0.05%; 0.2% indicates colony diameter of 6.8 cm; 5 cm; 3.7 cm (Aspergillus niger), 6.7 cm; 5 cm; 4.3 cm (Aspergillus flavus), 7 cm; 5 cm; 2.3 cm (Aspergillus fumigatus). Conclusion: Media with Addition of Clove Powder (Syzygium aromaticum) can inhibit the growth of Malassezia furfur and Aspergillus sp.

Citrus Hystrix D.C Fluid Inhibits the Growth of Escherichia Coli, Pseudomonas aerogenosa, and Bacillus subtilis Haitami1 , Leka Lutpiatina1 , Akhmad Muhlisin1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10782

Background: Citrus hystrix D.C is a plant traditionally used for herbs and medicinal plants in Indonesia. Citrus hystrix D.C fluid is proven to inhibit the growth of Staphylococcus aureus. The purpose of this study was to determine the ability of the fluid of Citrus hystrix D.C concentrations of 25%, 50%, 75%, and 100% in inhibiting the growth of Escherichia Coli, Pseudomonas aerogenosa, Bacillus subtilis. Material and Method: This research is experimental with Posttest Only Control Group Design. The material used was local Citrus hystrix D.C, fresh and clean green, obtained from the Astambul area, Banjar Regency, South Kalimantan Indonesia. Results: The results showed that there was a zone of inhibition of Citrus hystrix D.C fluid at a concentration of 25%, 50%, 75%, and 100% on the growth of Escherichia Coli, amounting to 7mm, 10mm, 13mm, 15mm. Pseudomonas aerogenosa, amounting to 6mm, 9mm, 13mm, 14mm. Bacillus subtilis, amounting to 6mm, 8mm, 11mm, 14mm. Conclusion: It concluded that the fluid of Citrus hystrix D.C affected the growth of Escherichia Coli, Pseudomonas aerogenosa, Bacillus subtilis with the most significant inhibition zones respectively 15mm, 14mm, and 14mm.

EFEKTIVITAS EKSTRAK ETANOL DAUN KIRINYUH (CHROMOLAENA ODORATA L) SEBAGAI ANTIBAKTERI SALMONELLA TYPHI DAN STAPHYLOCOCCUS AUREUS Fadia, Fadia; Nurlailah, Nurlailah; Helmiah, Tini Elyn; Lutpiatina, Leka
Jurnal Riset Kefarmasian Indonesia Vol 2 No 3 (2020): Jurnal Riset Kefarmasian Indonesia
Publisher : APDFI (Asosiasi Pendidikan Diploma Farmasi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33759/jrki.v2i3.104

Salmonella typhi and Staphylococcus aureus are pathogenic bacteria that cause infectious diseases. Treatment of these diseases can be done by giving antibacterial drugs. One of the plants that can be used as an antibacterial drug is the leaves of Chromolaena odorata L. due to the presence of chemical compounds such as flavonoids, tannins, and saponins that have the potential as antibacterial. This study aims to determine the Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) ethanol extract of Chromolaena odorata L. leaves on the growth of Salmonella typhi and Staphylococcus aureus. This research is the initial stage of the development of herbal medicines to treat Salmonella typhi and Staphylococcus aureus infections. The study consisted of 5 treatments, namely concentrations of 20%, 40%, 60%, 80%, and 100% ethanol extract of Chromolaena odorata L. leaves with five repetitions. The antibacterial activity test was carried out by MIC and MBC methods. The research material uses leaves from Chromolaena odorata L. in the Sungai Besar area, Banjarbaru, South Kalimantan, Indonesia. The results showed that the average MIC yield of ethanol extract of Chromolaena odorata L. leaves against Salmonella typhi: 20% and Staphylococcus aureus: 20%. While the average yield of MBC values â€‹â€‹against Salmonella typhi: 40% and Staphylococcus aureus: 40%. Based on the results obtained, it can be concluded that the ethanol extract of Chromolaena odorata L. leaves has an inhibitory effect on the growth of Salmonella typhi and Staphylococcus aureus. Chromolaena odorata L. leaves has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

Lime Soap as Bacteria Reducer Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Some people in South Kalimantan have used river water to fulfill their daily needs include washing the utensils. Wash the cutlery use river water can cause disease transmission. Squeezed lime has an antibacterial effect in inhibiting bacterial growth. Antibacterial dishwashing soap is effective in reducing pathogenic bacteria. This research is to know the difference of bacterial amount on the variation of contact time with soap. Know the difference of bacteria on the variation of concentration of lime soap and soap without lime. The study used the experimental method with pretest and posttest with control design. The independent variables are a lime soap with a concentration of 40%, 50%, 60% and variable of contact time 30 second, 60 second and 90 seconds. The dependent variable is the number of bacteria on the dinnerware of a dinner plate. The data obtained in this study is the data of measurement of the number of bacteria on the dinner plate. Data analyzed by Kruskal-Wallis test. MPN coliform examination of river water showed results >2400 CFU/ml sample. There were no significant differences in treatment with contact time variation of 30 seconds, 60 seconds and 90 seconds. The significance value is 0.796 (> 0.05). There were significant differences in the treatment of lime soap with concentration variations of 40%, 50%, 60%, and soap without lime. The value of significance is 0,000 (<0.05).

Potensi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Menghambat Pertumbuhan Candida albicans Annisa Rahmi; Erpan Roebiakto; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Candida albicans infection is the cause of candidiasis. Candidiasis treatment can be done with a variety of antifungal drugs, one of them is rhizome of kencur (Kaempferia galanga L.). The Rhizome of kencur is selected as a traditional medicine because it contains chemical compounds such as flavonoids, tannins, saponins and essential oil that serves as an antifungal. This study aimed to determine the minimal inhibitory and minimal killing power and also an influence of kencur rhizome extract on the growth of Candida albicans in vitro. This research was true experimental design with posttest only control group design with tube dilution method. Results of Minimal Inhibitory Concentrations (MICs) research showed there was no clarity at concentration of 20 mg/mL, 30 mg/mL, 40 mg/mL, and it shows clarity at concentration of 50 mg/mL and 60 mg/mL. Results of Minimum Bactericidal Concentrations (MBCs) showed the number of colonies at concentration of 20 mg/mL were 84 colonies, concentration of 30 mg/mL were 48 colonies, concentration of 40 mg/mL were 27 colonies, concentration of mg/mL were 12 colonies and concentration of 60 mg/mL were 0 colony. Based on linear regression test, the result showed significance value of 0.000

Mutu Bakteriologis Saus Tomat Pentol di Banjarbaru Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 2 No. 1 (2016): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstract: The food was used as a complementary flavor sauces include snacks bulb. Dressing materials according to SNI 01-7388-2009 must comply with the requirements of the parameter Staphylococcus aureus, MPN (Most probable number) Coliform, TPC (total plate count) and molds. This study aims to determine the percentage of tomato sauce snacks bulb in the area around the intersection of four Banjarbaru who do not qualify SNI 01-7388-2009 of parameters MPN Coliform and Staphylococcus aureus. The results showed MPN Coliform tomato sauce ranges from 0-240 and Staphylococcus aureus 5 x 101 to 6.5 x 103. Concluded percentage tomato sauce snacks bulb that does not qualify SNI 01-7388-2009 parameter is 60% MPN Coliform and Staphylococcus aureus 80%. Suggestions to determine the value of TPC (total plate count) and molds and other chemical parameters of tomato sauce.

Pewarnaan Gram Buffy Coat untuk Deteksi Awal Pasien Bakteremia leka lutpiatina
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstract: Bacteremia is one public health problem. Bacteremia was ranked as the tenth leading cause of death in the United States. Gram staining of buffy coat can be used for early detection of patients with bacteremia. Rapid diagnostic tests with these Gram staining can be interpreted within an hour and the research that has been conducted Richmond et al. 2002, have a sensitivity of 75% and specificity of 79%. This study aimed to study the sensitivity and specificity of Gram buffy coat with the gold standard blood culture media biphasic fever suspected patients. This study was an observational study with cross sectional study. The study population was suspected fever patients in hospitals Ratu Zalecha Martapura months from March to May 2013. The samples were suspect fever patients in hospitals Queen Zalecha Martapura March-May 2013 were taken by purposive with the inclusion criteria: Patients District General Hospital Ratu Zalecha Martapura, aged ≥10 years, had fever ≥ 5 days, had symptoms of abdominal pain, willing to follow the study. Gram staining microscopic results were analyzed by application epicalc. Microscopic results buffy coat Gram staining Gram-negative rods are found as many as seven (26%) and were not found Gram-negative rods as many as 20 (74%). Culture results of this study are Gram negative bacterial growth as much as 6 (22%) and no growth as many as 21 (78%). Conclusion The results of microscopic Gram stain buffy coat has a value of 67% sensitivity and 86% specificity.

Pengaruh Variasi Suhu Awal Reagen terhadap Kadar Glukosa Darah Metode Enzimatik Yayuk Kustiningsih; Nastiti Megawati; Jasmadi Joko Kartiko; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 3 No. 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Pre-analysis phase is the first step that determines the quality of the examination results, in this case, is the preparation of glucose work reagent. Glucose is one of the clinical examination clinical parameters that are often done in the laboratory. This glucose examination uses enzymatic methods. Enzyme activity is influenced by several factors, namely temperature, pH, substrate level, enzyme level, and inhibitor. Glucose examination performed at a temperature of 20-25oC or 37oC, but there are still laboratories in the field that is less attention to the initial temperature conditions of this reagent. This study aims to determine the effect of initial temperature variations of reagents on blood glucose levels of enzymatic methods. The research used Quasi Experimental research method with Posttest-Only Control Group Design design. The sample of this research used serum of a respondent who had no history of Diabetes Mellitus disease with 4 replication at each treatment. The results of the study with the initial temperature of 10oC, 13oC, 16oC, 19oC, 22oC and 25oC (control) respectively of 62.71 mg / dL; 65,74 mg / dL; 72.45 mg / dL; 76.91 mg / dL; 89.12 mg / dL and 97.19 mg / dL, the results of this study showed an increase in glucose levels in each treatment. Based on the results of linear regression analysis obtained a significance value of 0.000 with α = 0.05, which means Ho is rejected, so it can be concluded that there is an influence of initial temperature variation of reagent on blood glucose level. For the next researcher can do research about the effect of the initial temperature of reagent 22-38 oC on blood glucose enzymatic method. And the influence of incubation time on blood glucose level of enzymatic method.

Staphylococcus aureus and Methicillin resistant Staphylococcus aureus From The Diabetic Ulcer Leka Lutpiatina; Nisa Nur Agistni Eriana
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Infections of diabetic ulcers may occur because patients with diabetes mellitus have a weak immune system and the presence of high blood sugar into a strategic place for bacterial growth one of them is Staphylococcus aureus. Increased resistance of Staphylococcus aureus to various antibiotics, causing problems for the treatment of this infection. As by Methicillin-resistant Staphylococcus aureus (MRSA) This study aims to assess the presence of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) at diabetic ulcer patient at Banjarmasin city hospital. This research uses descriptive observational research type. The study sample was patients with grade 2 and three diabetic ulcers with a total of 30 samples. The result of the research found 14 Staphylococcus aureus bacteria, Staphylococcus epidermidis nine, Staphylococcus saprophyticus two and 5 Gram-negative bacteria. Of the 14 samples of Staphylococcus aureus, there were eight samples of cefoxitin-resistant samples. The conclusion was 47% of Staphylococcus aureus in patients with a diabetic ulcer at Banjarmasin City Hospital. There was a 57% Methicillin-resistant Staphylococcus aureus in the isolated Staphylococcus aureus found. Need further research on the resistance of antibiotics oxacillin and cefoxitin to other Staphylococcus sp bacteria derived from patient diabetic ulcers.

Co-Authors Abdul Khair Ahmad Muhlisin Annisa Nur Yulianti Annisa Rahmi Anny Thuraidah Dinna Rakhmina Elfira Maulidah Erpan Roebiakto Fadia, Fadia Farah Indah Swari Gina Alia Mawaddah hana nailah Helmiah, Tini Elyn jasmadi Joko Kartiko Muhammad Ma'ruf Nastiti Megawati Nisa Nur Agistni Eriana Noraida Noraida Nur Rizqi Amaliah Nurhilaliah Nurhilaliah Nurlailah Nurlailah, Nurlailah Raimunah Raimunah Ratih Dewi Dwiyanti Rifqoh Rifqoh Syaidatul Aslamiyah wahdah norsiah Wahidah Wahidah Widiyawati Widiyawati Yayuk Kustiningsih

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