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All Journal Jurnal Kimia (Journal of Chemistry)
Darma Asih Yuliana
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Chemistry

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ISOLASI DNA METAGENOMIK DARI TANAH HUTAN MANGROVE PANTAI SUWUNG BALI I Nengah Wirajana; Darma Asih Yuliana; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 1 Januari 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.666 KB) | DOI: 10.24843/JCHEM.2013.v07.i01.p03

Abstract

Metagenomic DNA isolation from mangrove forest soils of Suwung Beach-Bali was conducted to exploit the biocatalytic potential of microbial communities for the discovery of novel cellulase. The whole DNA isolation was conducted by modification of preparation step by Marco (2010) and DNA extraction step by Amorim et al (2008). The results of metagenomic DNA isolation were analyzed by spectrophotometry and agarose gel electrophoresis. The results showed that whole DNA was able be isolated successfully, but protein and humic acid were found as contaminant.
SKRINING SELULASE DARI TANAH HUTAN MANGROVE PANTAI SUWUNG BALI I Nengah Wirajana; Ketut Ratnayani; Darma Asih Yuliana
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 2 Juli 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.478 KB)

Abstract

Mangrove soil has high biodiversity and well known as potential location for enzymes exploration. The aim of direct screening for cellulase of mangrove soil is to find out the cellulase activity from mangrove soil. Mangrove soils were collected from three different locations labelled as A (8o43’38.20”SL), B (8o43’46,18”SL), and C (8o43’37,38”SL). The screening was conducted by Filter Paper Assay and Carboxymethyl Cellulose Assay methods. The results showed that cellulase activities can be measured directly from mangrove soil samples of Suwung Beach-Bali. The highest cellulase activities were 0,866 U/g soil by Filter Paper Assay and4,176 ± 0,630 U/g soil by Carboxymethyl Cellulose Assay, given by soil samples C.
Co-Authors I Nengah Wirajana Ketut Ratnayani