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All Journal Titian Ilmu: Jurnal Ilmiah Multi Sciences Meditory : The Journal of Medical Laboratory Jurnal Kesehatan Prima Jurnal Analis Medika Biosains (JAMBS) Jurnal Gizi Prima
Ari Khusuma
Poltekkes Kemenkes Mataram
Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Nursing Public Health

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Variasi Temperatur Boiling pada Amplifikasi Gen inhA M.tuberculosis Metode PCR Fihiruddin Fihiruddin; Hanifa Falahul Ilmi; Ari Khusuma
Titian Ilmu: Jurnal Ilmiah Multi Sciences Vol. 14 No. 2 (2022): July Edition
Publisher : Universitas Nurul Huda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30599/jti.v14i2.1661

Abstract

Polymerase Chain Reaction (PCR) adalah metode yang direkomendasikan oleh world health organization (WHO) untuk pemeriksaan infeksi tuberkulosis dengan sensitivitas dan spesifisitas yang tinggi. Biaya yang murah, proses yang mudah, dan tehnik ekstraksi DNA yang cepat sangat diperlukan untuk mendapatkan DNA template. Salah satu cara untuk mendapatkan DNA template adalah metode boiling, dimana metode ini dapat meningkatkan permeabilitas dan merusak dinding sel hanya dengan pemanasan dalam waktu relatif singkat. Penelitian ini bertujuan untuk mengetahui pengaruh variasi suhu boiling 85°C, 90°C, dan 95°C masing-masing selama 30 menit, 10 menit dan 5 menit terhadap hasil amplifikasi gen inhA M. tuberculosis menggunakan metode PCR. Penelitian ini merupakan penelitian True Experiment dengan rancangan posttest only control design. Sampel yang digunakan dalam penelitian ini adalah dahak penderita TB yang di ekstraksi dengan metode boiling dan dibandingkan dengan metode ekstraksi kit. Hasil amplifikasi di elektroforesis pada gel agarosa 1% yang diamati dengan alat UV transiluminator. Hasil penelitian menunjukkan bahwa ekstraksi sampel sputum dengan boiling pada suhu  850C selama 30 menit, 900C selama 10 menit dan 950C selama 5 menit masing-masing ditemukan pita DNA gen inhA M.tuberculosis dengan panjang 465 bp. Ekstraksi sampel sputum dengan metode boiling dapat digunakan untuk pemeriksaan gen inhA M.tuberculosis metode PCR.
Intervention Of Ambon Banana Blossom As An Alternative Food To Reduce Blood Glucose Levels In Diabetic Patients In The Working Area Of Kemalo Abung Health Center Ari Khusuma; Annisa Agata; Arini Pradita Roselyn; Annisa Mulia Annasis
Jurnal Gizi Prima (Prime Nutrition Journal) Vol 8, No 1 (2023): Jurnal Gizi Prima (Prime Nutrition Journal)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jgp.v8i1.385

Abstract

Background: The prevalence of diabetes in Indonesia based on the results of the Riskesdas in 2013 is 2.1%; the figure is higher compared with the year 2007 (1.1%). Complications in people with diabetes occur due to uncontrolled blood glucose increases. The necessity of controlling glucose is easily obtainable and cheap, such as granting high fiber with Ambon's Banana as in Pringsewu Regency. This research aims to know the influence of consuming refined Ambon's Banana blossom against the decrease in blood glucose levels in people with diabetes. Research Methods: Types of quantitative research and analysis by a quasi-experimental approach. The population in this research is a citizen who lived in the region of clinics Abung Kemalo Kab. North Lampung affected by diabetes mellitus amounted to 116 people. Sample 10 people. Analysis of a t-test using the bivariate test. Research Result: The results showed the average blood glucose level before the banana blossom given to the intervention group was 224.4. While in the group, the control was 280.4. After the banana blossom preparations were given in the intervention group, average blood glucose levels were 172. At the same time, the control group was 251.0. Conclusion: There is a difference between consuming the refined Ambon's banana blossom and not consuming the refined Ambon's banana against the decrease in blood glucose levels in people with diabetes (p-value 0.022). This research is expected to advise health workers should be socialized heart Ambon's banana as an alternative for patients with DM.
Co-Authors Agrijanti Agrijanti Alfiah Novianty Annisa Agata Annisa Agata Annisa Agata Annisa Agata Annisa Mulia Anasis Annisa Mulia Annasis Annisa Yuniarni Arini Pradita Roselyin Arini Pradita Roselyn Arini Pradita Roselyn Dewi, Lale Budi Kusuma Fihiruddin Fihiruddin Hanifa Falahul Ilmi Kurnia Rizki Meidiana Intan Suryawatie Yomo Ni Putu Winda Siti zaetun Suhartiningsih Suhartiningsih Wiodi Nazhofatunnisa Umami Yudha Anggit Jiwantoro Yuriska Safitri