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All Journal HAYATI Journal of Biosciences Indonesian Journal of Agricultural Science Floribunda Dinamika Kerajinan dan Batik : MAJALAH ILMIAH
Alex Hartana
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences

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 1 
Phylogenetic Study of Mangifera laurina and its Related Species Using cpDNA trnL-F Spacer Markers . Fitmawati; Alex Hartana
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.873 KB) | DOI: 10.4308/hjb.17.1.9

Abstract

Phylogenetic study of cpDNA intergenic spacer trnL-F of Mangifera laurina and their related species within the genus Mangifera in Indonesia was conducted using Rutaceae as the outgroup. This study was to reconstruct phylogenetic relationships and to understand infraspecific relationships within Mangifera based on cpDNA trnL-F intergenic spacer sequences. The results showed that Mangifera sp. Hiku (mangga hiku) as the basic cultivar in the clade, and it supported the monophyletic group in Mangifera. And phylogenetic construction indicated that Mangifera sp. Hiku was the progenitor of M. laurina and their related species.
THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE Satoto Satoto; Yuli Sulistyowati; Alex Hartana; Inez H. Slamet -Loedin
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v9n2.2008.p35-43

Abstract

Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2) of fivetransgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. Data  resulted from PCR of F2 population were analysed using a Chi Square test. The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of genetic sources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer or  separately in rice breeding programs whenever the efficacy tests and biosafety requirements have been completed.
GENETIC DIVERSITY OF PANDANUS AND FREYCINETIA FROM JAVA BASED ON ISSR MARKER Sri Endarti Rahayu; Alex Hartana; Tatiek Chikmawati; Kuswata Kartawinata
Floribunda Vol. 3 No. 3-4 (2007)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6154.507 KB) | DOI: 10.32556/floribunda.v3i3-4.2007.76

Abstract

Keragaman genetik pandanus dan Freycinetia dari Jawa berdasarkan penanda ISSR.
DISTRIBUSI KAPULASAN (NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.) DI PULAU JAWA DAN HUBUNGAN KEKERABATAN MORFOLOGINYA Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 5 No. 4 (2016)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.262 KB) | DOI: 10.32556/floribunda.v5i4.2016.133

Abstract

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribution of pulasan [Nephelium  ramboutan-ake (Labill.) Leenh] in Java and Relationship of Their Morphology. Floribunda 5(4): 129–138. — Distribution of pulasan in Java is not known yet. The purposes of this study were to provide information about the locations of pulasan trees in Java, and to analyze relationship of this species based on morphological characters. Pulasan exploration was carried out in four provinces in Java encompassed West Java, Central Java, East Java, and Yogyakarta Special Region. Morphological characteristics of pulasan were observed, then the relationships were analyze using NTSys pc 2.02 program.  Result showed that most of pulasan located in West Java, especially in the area of Bogor.  Based on the dendrogram, it was known that the use of ve-getative dan generative characters could distinguish males and hermaphrodite pulasan trees, while the use of vegetative characters only, could not differentiate the type of pulasan trees. Pulasan trees from different areas can be grouped in the same cluster because of the similarity of their morphological traits.Keywords: Kapulasan, morphological characters, dendrogram, Sapindaceae. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribusi Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Pulau Jawa dan Hubungan Kekerabatan Morfologinya. Floribunda 5(4): 129–138. — Persebaran kapulasan di Pulau Jawa belum diketahui dengan pasti.  Penelitian ini bertujuan memberikan informasi tentang lokasi-lokasi yang masih mempunyai pohon kapulasan di Pulau Jawa, dan menganalisis hubungan kekerabatannya berdasarkan ciri morfologi. Eksplorasi kapulasan dilakukan di empat provinsi di Pulau Jawa yaitu Jawa Barat, Jawa Tengah, Jawa Timur, dan Daerah Istimewa Yogyakarta. Tanaman hasil eksplorasi diamati ciri morfologinya, kemudian dianalisis hubungan kekerabatannya dengan menggunakan program NTSys pc 2.02.  Hasil penelitian menunjukkan bahwa sebagian besar kapulasan terdapat di Jawa Barat terutama di daerah Bogor. Berdasarkan dendrogram yang dihasilkan, diketahui bahwa penggunaan ciri  vegetatif dan generatif dapat membedakan kapulasan jantan dan hermafrodit, sedangkan penggunaan ciri  vegetatif saja, tidak dapat membedakan kedua tipe tanaman tersebut. Tanaman kapulasan dari daerah yang berbeda dapat mengelompok dalam satu grup yang sama karena adanya keserupaan dalam ciri-ciri morfologinya.Kata kunci: Kapulasan, karakter morfologi, dendrogram, Sapindaceae. 
PEMANFAATAN DAUN HARENDONG (Melastoma malabathricum) SEBAGAI PEWARNA ALAMI UNTUK KAIN KATUN Enur Azizah; Alex Hartana
Dinamika Kerajinan dan Batik: Majalah Ilmiah Vol 35, No 1 (2018): Dinamika Kerajinan dan Batik : Majalah Ilmiah
Publisher : Balai Besar Kerajinan dan Batik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22322/dkb.v35i1.3490

Abstract

Meningkatnya kesadaran di kalangan masyarakat tentang efek bahaya dari penggunaan zat pewarna sintetis membuat eksplorasi zat pewarna alami terus dilakukan. Banyak pewarna yang dihasilkan dari tumbuhan dan digunakan untuk pewarnaan tekstil. Penelitian dilakukan untuk mencari potensi dari daun harendong (Melastoma malabathricum) dalam menghasilkan pewarna alami untuk kain katun. Optimalisasi pewarnaan kain dilakukan dengan penambahan mordan berupa tawas [KAl(SO4)2.I2H2O], kapur sirih (Ca(OH)2), dan tunjung (FeSO4) saat proses fiksasi pada kain katun. Kain katun yang telah diwarnai diidentifikasi warnanya menggunakan RGB (Red Green Blue) Color Chart Reader. Kain katun yang diwarnai dengan ekstrak daun harendong menghasilkan warna Banana (#E3CF57). Pemberian mordan saat fiksasi berpengaruh terhadap warna kain. Kain yang difiksasi menggunakan tawas berwarna Khaki 1 (#FFF68F), sedangkan yang difiksasi menggunakan kapur sirih berwarna Lightgoldenrod 1 (#FFEC8B) dan kain yang difiksasi menggunakan tunjung berwarna Sgi Gray 36 (#5B5B5B). Penambahan mordan juga memberikan pengaruh baik terhadap ketahanan luntur warna pada kain katun akibat pengaruh pencucian dan paparan sinar matahari. Daun harendong berpotensi baik dalam mewarnai kain katun.
Co-Authors . Fitmawati Dorly Dorly Enur Azizah Inez H. Slamet -Loedin Kuswata Kartawinata Nina Ratna Djuita Satoto Satoto Sri Endarti Rahayu Tatiek Chikmawati TATIK CHIKMAWATI Yuli Sulistyowati