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All Journal HAYATI Journal of Biosciences AGRIVITA, Journal of Agricultural Science
GEDE SUASTIKA
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Identification and Molecular Characterization of Odontoglosum Ringspot Virus (ORSV) from Bogor, Indonesia IRWAN LAKANI; GEDE SUASTIKA; NURHAJATI MATTJIK; TRI ASMIRA DAMAYANTI
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.571 KB) | DOI: 10.4308/hjb.17.2.101

Abstract

Recently, field surveys were conducted in several orchid nurseries in Bogor (West Java), Magelang (Central Java) and Malang (East Java). We found that most of the commercially orchids sampled from Bogor was infected by virus-like disease. The symptoms depend on the orchid species. Thus, the symptoms varied such as mottle, mosaic and necrotic flecks either on the leaves or on the flowers. Similiar symptoms were not found in samples obtained from Central Java and East Java. A Tobamo-like virus was inferred to be possible cause of the viral disease-like symptoms. Serological test of the samples by ELISA showed positive against Odontoglosum Ringspot Virus (ORSV) antibody and was negative against Cymbidium Mosaic Virus (CyMV) antibody. Total RNA was extracted from symptomatic plants and RT-PCR was carried out by using a pair of coat protein (CP) gene primer of ORSV.  It was successfully amplified a 500 bp of DNA fragment and it was directly sequenced. The nucleotide sequence of CP gene had confirmed the identity of ORSV. Phylogenetic analysis based on the CP nucleotide sequences were grouped into only one major cluster.  The ORSV isolate from Bogor (Sd 21) and the other isolates were clustered in the same group and had highest nucleotide homology (99%). These results provide first evidence of ORSV infecting orchids in Bogor, Indonesia.
IDENTIFICATION AND CHARACTERIZATION OF CARMOVIRUS ON CARNATION (Dianthus caryophyllus L.) IN WEST JAVA, INDONESIA Erniawati Diningsih; Gede Suastika; Tri Asmira Damayanti; Slamet Susanto
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i2.523

Abstract

Carnation has a highly economic demand of cut flower in Indonesia.  Field observations in West Java Indonesia was conducted in order to find the typical mottle symptoms that was a suspect caused by a virus disease. Identification of the virus was respectively conducted by performing ELISA test with four anti sera and characterizations held by bioassay, observing of virion particles, detecting of nucleic acid by RT-PCR and nucleotide sequencing. Total of 403 samples were collected from plants with or no virus-like symptoms. Among those all tested, 83% were found to be infected by Carnation mottle virus (CarMV), but negatively against Carnation ringspot virus (CRSV), Carnation laten virus (CLV), and Carnation vein mottle virus (CVMV) antisera. By mechanical inoculation, the virus was able to infect systemically Cenopodium quinoa and locally infect on others. However on Phalaenopsis sp and Gomprena globosa, there was symptompless found. The isometric CarMV particles size was approximately 30 nm. RT-PCR using specific primers of CP gene of CarMV successfully amplified a DNA sized 1000 bp.  CarMV West Java Indonesian (Idn-WJ) isolates possessed the highest nucleotide and amino acid homology with CarMV from Spain and was in the same cluster with CarMV from China, Taiwan and Israel.
Co-Authors Erniawati Diningsih IRWAN LAKANI NURHAJATI MATTJIK Slamet Susanto TRI ASMIRA DAMAYANTI