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All Journal HAYATI Journal of Biosciences
AMANDA KRISTANI
Department of Biology, Faculty of Biotechnology, Atma Jaya Catholic University
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Diversity of Amylase-Producing Bacillus spp. from “Tape” (Fermented Cassava) TATI BARUS; AMANDA KRISTANI; ADI YULANDI
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.662 KB) | DOI: 10.4308/hjb.20.2.94

Abstract

Fermented cassava or “Tape” is one of traditional Indonesian fermented food. The quality of “Tape” is determined by microorganisms involved during fermentation process. It was reported that Bacillus subtilis determined the quality of cassava “Tape”. The most common way to identify species is by using 16S rRNA gene.  This gene contains conserved regions as unique sequence which is relative among species. It has been widely used as a reliable molecular marker for phylogeny identification. Therefore, the aim of this research was to study diversity of amylase-producing Bacillus spp. from “Tape” based on 16S rRNA gene sequences. Bacillus spp. were isolated from “Tape” from several area in Indonesia i.e. Jakarta, Bandung, Cianjur, Subang, Rangkas Bitung, and Kediri. Amplification of 16S rRNA gene used 63f and 1387r primers. This research showed that based on 16S rRNA gene sequences, twenty-six of amylase-producing Bacillus spp. isolates were divided into four groups. All isolates were identified as species either B. megaterium, B. subtilis, B. amyloliquefaciens, or B. thuringiensis.
Co-Authors ADI YULANDI Tati Barus