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All Journal HAYATI Journal of Biosciences Jurnal INKOM
SITI NURHAYATI
Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency of Indonesia
Agriculture, Biological Sciences & Forestry Computer Science & IT Earth & Planetary Sciences

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Liver Histophatological Studies of Mice (Mus musculus sp.) Infected With Gamma Rays Irradiated Plasmodium berghei Strains Anka TUR RAHARDJO; SITI NURHAYATI; DWI RAMADHANI
HAYATI Journal of Biosciences Vol. 20 No. 3 (2013): September 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.115 KB) | DOI: 10.4308/hjb.20.3.144

Abstract

Malaria is a serious global disease and a leading cause of morbidity and mortality in tropical and sub-tropical countries. Radiation attenuated vaccines have been demonstrated to be an effective means of controlling certain parasitic infections such as malaria. Our previous research showed that irradiation dose of 150-175 Gy to Plasmodium berghei were enable to suppress the infected erythrocyte cells and irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on parasitemia index. Aim of this research was to investigate the most suitable irradiation dose to attenuated P. berghei based on the the histological changes in the liver mouse malaria model. Histological changes in mice liver because of Plasmodium is congestion in the sinusoids and masses of hemozoin. Fourty Swiss mice were randomly assigned to four equal groups. First group of mice were inoculated with the unirradiated malaria parasites as a positive control while negative control group represented uninfected normal control. The third group of mice was inoculated with the irradiated malaria parasites with dose 175 Gy. Last group were inoculated with the irradiated malaria parasites with dose 150 Gy. Mice were sacrificed at day 6, 8, 12, 16, 20, and 24 post inoculation for liver histological study. Their livers processed for histomorphometric assessment of sinusoidal area, haemozoin area and hepatosomatic index as a quantitative measure of altered morphology. Hepatic sinusoidal area in mice infected with the unirradiated malaria parasites increased by 104% in day 24 post inoculated compared to day 6 post inoculated, whereas a smaller increase of 46% occurred in mice that were inoculated with the irradiated malaria parasites with dose 150 Gy. The difference between positive control and 150 Gy group was significant (P < 0.05).  The highest average haemozoin area in all days post inoculation was in positive control whereas a smaller occurred in mice that were inoculated with the irradiated malaria parasites with dose 175 Gy, but the different was not significant (P > 0.05). For hepatosomatic index (HSI) at day 24 post inoculation the HSI value in group 150 Gy (5.33) was the smallest compared to other groups. Based on our research it can be concluded that irradiation dose at 150 Gy was more effective compared to 175 Gy in attenuate the parasites based on assessment of sinusoidal area, and HSI value at 24 day post inoculation.
Automated Estimation Parasitemia of Plasmodium berghei Infected Mice using CellProfiler Software Dwi Ramadhani; Siti Nurhayati
INKOM Journal Vol 7, No 2 (2013)
Publisher : Pusat Penelitian Informatika - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/j.inkom.263

Abstract

In this paper, we propose a technique for automatically recording parasitemia of mice infected with Plasmodium berghei by using Cellprofiler. Our purpose is to identify the difference number of parasitemia obtained by CellProfiler and manual assessment. We conducted a T-test analysis with p < 0.05. This value is considered to have a statistically significant different between automatic and manual process. Total of 50 thin blood smear images were analyzed for both automatically using CellProfiler and manual process. Results showed that there were insignificant difference between automatic and manual process (p > 0.05). It can be concluded that based on this research that automated quantification of parasitemia using CellProfiler was comparable but not better than manual.keywords: Automation, CellProfiler, Parasitemia, Plasmodium berghei, Thin blood smearPada tulisan ini, penghitungan parasitemia pada mencit yang diinfeksi dengan Plasmodium berghei dengan CellProfiler diusulkan. Tujuan penelitian yang dilakukan adalah untuk mengetahui apakah terdapat perbedaan antara nilai parasitemia yang diperoleh dengan menggunakan CellProfiler dibandingkan dengan secara manual. Uji T digunakan untuk analisis statistik dengan p < 0, 05 berarti terdapat perbedaan yang signifikan antara penghitungan otomatis dibandingkan dengan manual. Total sebanyak lima puluh citra apusan darah tipis mencit dianalisis secara otomatis menggunakan CellProfiler dan secara manual. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan signifikan antara nilai persentase yang diperoleh secara otomatis dibandingkan dengan manual (p > 0, 05) . Dengan demikian dapat disimpulkan bahwa nilai persentase parasitemia yang diperoleh secara otomatis menggunakan CellProfiler sebanding tetapi tidak lebih baik dibandingkan dengan teknik manual.kata kunci: CellProfiler, Otomatisasi, Parasitemia, Plasmodium berghei, Preparat Apusan Tipis
Co-Authors DWI RAMADHANI TUR RAHARDJO