Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences
I Nengah Budiarsa
Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells Silmi Mariya; Fitriya Nur Annisa Dewi; Irma Herawati Suparto; Gregory K. Wilkerson; J. Mark Cline; . Permanawati; Diah Iskandriati; I Nengah Budiarsa; Dondin Sajuthi
HAYATI Journal of Biosciences Vol. 24 No. 3 (2017): July 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1422.555 KB) | DOI: 10.4308/hjb.24.3.136

Abstract

The mammary gland contains adult stem cells that are capable of self-renewal and are likely target for neoplastic transformation leading to breast cancer. In this study, we developed a cell culture derived from the mammary glands of cynomolgus monkeys (Macaca fascicularis)(MfMC) and furthermore identified the expression of markers for stemness and estrogenreceptor-associated activities. We found that the primary culture can be successfully subcultured to at least 3 passages, primarily epithelial-like in morphology, the cultured cells remained heterogenous in phenotype as they expressed epithelial cell markers CD24, CK18, and marker for fibroblast S1004A. Importantly, the cell population also consistently expressed the markers of mammary stem cells (ITGB1 or CD29 and ITGA6 or CD49f), mesenchymal stem cells (CD73 and CD105) and pluripotency (NANOG, OCT4, SOX2). In addition to this, the cells were also positive for Estrogen Receptor (ER), and ER-activated marker Trefoil Factor 1, suggesting an estrogen responsiveness of the culture model. These results indicate that our cell culture model is a reliable model for acquiring a population of cells with mammary stem cell properties and that these cultures may also serve as a reservoir from which more purified populations of stem cell populations can be isolated in the future.
Co-Authors . Permanawati Dewi, Fitriya Nur Annisa Diah Iskandriati Dondin Sajuthi Gregory K. Wilkerson Irma Herawati Suparto J. Mark Cline Silmi Mariya