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Dondin Sajuthi
Departemen Klinik, Reproduksi Dan Patologi, Fakultas Kedokteran Hewan, IPB University
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Antioksidan Beluntas Wijaya, C. Hany; Hardjosworo, Peni Suprapti; Sajuthi, Dondin
REKAPANGAN Vol 5, No 1 (2011): REKAPANGAN
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Beluntas  (Pluchea  indica  Less)  is  a  herb  that  commonly  used  as  traditional medicine  and  food.    Its  leaves  have  many  biological  activities,  because  they  contain various  phenolic  compounds.  The  phenol  concentration  of  beluntas  leaves  depends on leaf segment, earth condition, fertilizer and environmental stress.  This research is focused  to  determine   antioxidant  activity  of  three  groups  of    beluntas  leaf  segments that   includes 1-3, 4-6 and > 6.  They were ground, dried at ambient temperature for  7  days, maserated by petroleum eter (1:4 b/v) at ambient temperature for 24 hours and soxhlet  extracted  by  methanol  (1:15  b/v)  at  65oC  for  3   hours.  The  result  shows  that the  extract  contains  phytochemical   compounds  such  as  tannin,  sterol,  phenol hydroquinone  and  flavonoid.  This  extract  has  antioxidant  activity  to  scavenging  free radical  DPPH   (2,2-phenyl-1-picrylhydrazyl).  The extract of  1-3  beluntas  leaf  segment  was   more  potential  antioxidant  source  than  4-6  and  >  6  beluntas  leaf   segments,  because it had the least IC50 (inhibitor concentration) and the  highest total phenol and  total flavonoid. The IC50, total  phenol and flavonoid  total of  it were 3.71 mg/L,  234.65  mg GAE/100 g samples  and 2163.59 mg QE/ 100 g samples, respectively.
Kajian Pakan Bersumber Energi Tinggi pada Pembentukkan Monyet Obes Oktarina, Ria; Mansjoer, Sri Supraptini; Astuti, Dewi Apri; Suparto, Irma Herawati; Sajuthi, Dondin
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.126 KB) | DOI: 10.14203/jbi.v6i3.3145

Abstract

ABSTRACTHigh Energy Diet to Develop Obese Animal Model in Cynomolgus Monkeys (Macacafascicularis). Obesity is a primary predisposition for diseases such as metabolic syndrome(insulin resistance, blood lipid abnormality, and hypertension), type 2 diabetes, andcardiovascular disease. The effort to overcome obesity is needed by understanding thedevelopment of obesity. Therefore, it is urgent to carry out preclinical trials by using an obeseanimal model however it is still limited. The objective of this research was to study the effect ofhigh energy diet with animal fat and soluble carbohydrate to produce obese cynomolgusmonkeys (Macaca fascicularis). Animals used in this study were 15 adult males dividedequally into three treatment groups and were given diets for 4 months. The 3 groups were 1)receiving diet A consists of beef tallow without egg yolk; 2) receiving diet B consists of beeftallow and egg yolk, (energy 4,207 cal/g, fat 19.62%, and starch 60.34% in both first two diet) 3)receiving diet C consists of monkey chow as control (energy 4,330 cal/g, fat 5.55%, and starch51.38%). Measurement were taken every four weeks for body weight, crown rump length, hipdiameter, abdominal skin thickness, body mass index (BMI), nutrient consumption anddigestibility. Results showed that animals received diet B had significant increase in bodyweight and BMI at week 4 and 8. Hip diameter and abdominal skin thickness were significantstarting at week 4 in animals receiving diet with egg yolk (p<0.05) compared to the other twogroups. Protein consumption and digestibility in group with diet A and B was lower (p<0.05)compared to control animals. However, digestibility for dry matter, fat, starch and energy wereabout 90%, meaning that the rations were considered adequate in developing obese monkey.The diet formula containing tallow and egg yolk increased body weight based on BMI criteriaabove 25 kg/m2.Key words: obesity, cynomolgus, egg yolk, body mass index, digestibility
KAJIAN PAKAN BERSUMBER ENERGI TINGGI PADA PEMBENTUKKAN MONYET OBES Oktarina, Ria; Mansjoer, Sri Supraptini; Astuti, Dewi Apri; Suparto, Irma Herawati; Sajuthi, Dondin
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3145

Abstract

ABSTRACTHigh Energy Diet to Develop Obese Animal Model in Cynomolgus Monkeys (Macacafascicularis). Obesity is a primary predisposition for diseases such as metabolic syndrome(insulin resistance, blood lipid abnormality, and hypertension), type 2 diabetes, andcardiovascular disease. The effort to overcome obesity is needed by understanding thedevelopment of obesity. Therefore, it is urgent to carry out preclinical trials by using an obeseanimal model however it is still limited. The objective of this research was to study the effect ofhigh energy diet with animal fat and soluble carbohydrate to produce obese cynomolgusmonkeys (Macaca fascicularis). Animals used in this study were 15 adult males dividedequally into three treatment groups and were given diets for 4 months. The 3 groups were 1)receiving diet A consists of beef tallow without egg yolk; 2) receiving diet B consists of beeftallow and egg yolk, (energy 4,207 cal/g, fat 19.62%, and starch 60.34% in both first two diet) 3)receiving diet C consists of monkey chow as control (energy 4,330 cal/g, fat 5.55%, and starch51.38%). Measurement were taken every four weeks for body weight, crown rump length, hipdiameter, abdominal skin thickness, body mass index (BMI), nutrient consumption anddigestibility. Results showed that animals received diet B had significant increase in bodyweight and BMI at week 4 and 8. Hip diameter and abdominal skin thickness were significantstarting at week 4 in animals receiving diet with egg yolk (p<0.05) compared to the other twogroups. Protein consumption and digestibility in group with diet A and B was lower (p<0.05)compared to control animals. However, digestibility for dry matter, fat, starch and energy wereabout 90%, meaning that the rations were considered adequate in developing obese monkey.The diet formula containing tallow and egg yolk increased body weight based on BMI criteriaabove 25 kg/m2.Key words: obesity, cynomolgus, egg yolk, body mass index, digestibility
KAJIAN TINGKAH LAKU MONYET EKOR PANJANG (Macaca Fascicularis) OBES DALAM KANDANG INDIVIDU Pijoh, Deyv; Astuti, Dewi Apri; Mansjoer, Sri Supraptini; Sajuthi, Dondin; Suparto, Irma Herawati
ZOOTEC Vol 40, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.697 KB) | DOI: 10.35792/zot.40.2.2020.30455

Abstract

STUDY ON BEHAVIOUR OF THE LONG TAIL MONKEY (Macaca fascicularis) OBESE IN INDIVIDUAL CAGE. This study was designed to obtain information of development behaviour the obesity of long-tailed monkey (Macaca fascicularis) fed with high energy obes diet. The need of obese monkeys highly to model byomedis obesity. Obesity is now a universal problem that needs to be explored and studied. Therefore, the objective of this research was to develop obese adult male monkeys with high energy food for 12 months and monitoring their behaviour. Fifteen adult male monkeys 6-8 years old with the weight of between 4-5 kg were divided equally into three groups. The first group was body weight average 4.50 kg, the second group was body weight average 5.00 kg; and the third group was body weight average 4.75 kg. The results showed that body weight gave a significant effect (P <0.01) on daily behaviour and also gave a significant effect (P<0.01) on fed behaviour frequently,drink behaviour, agonistic behaviour, grooming behaviour, and locomotion behaviour .Keywords: Behaviour frequently, Long-tailed monkey (Macaca fascicularis), Obesity)
Diurnal Patterns of Testosterone and Cortisol Metabolites in Fecal of Javan Gibbons (Hylobates moloch) in Captivity PUDJI ASTUTI; TUTY LASWARDI YUSUF; ERIC HAYES; HERA MAHESHWARI; LUTHFIRALDA SJAHFIRDI; DONDIN SAJUTHI
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (78.12 KB) | DOI: 10.4308/hjb.13.2.69

Abstract

The aims of this research were to determine diurnal patterns of testosterone and cortisol metabolites to predict the testis functional status. In this study, fecal testosterone and cortisol were quantified in 77 samples from three male Hylobates moloch during a course of three months period. These data showed that the highest concentration of fecal testosterone occured at 18.00-06.00 (23.61 ng/g dried feces), then declined gradually. The lowest concentration was in the evening (5.54 ng/g dried feces). Our tests showed that there was a decrease in the mean testosterone concentration from 06.00-10.00 to 10.00-14.00 to 14.00-18.00. For cortisol, the highest concentration occured at 06.00-10.00 (597.84 ng/g dried feces), then decline gradually in the evening (225.73 ng/g dried feces). Key words: Hylobates moloch, feces, testosterone, cortisol, diurnal pattern
High carbohydrate as obese diet is not yet available commercially for monkeys. Therefore, this preliminary study was to carry out nutrient intake and digestibility of cynomolgus monkeys (Macaca fascicularis) fed with high soluble carbohydrate diet compared to monkey chow. Five adult female macaques (average body weight 2.67 kg) were made to consume freshly  diet. Commercial monkey chows (contains 3500 cal/g energy and 35% starch) were fed to three adult females (average body weight 3.62 kg). Nut DEWI APRI ASTUTI; IRMA HERAWATI SUPARTO; DONDIN SAJUTHI; I NENGAH BUDIARSA
HAYATI Journal of Biosciences Vol. 16 No. 4 (2009): December 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.16.4.147

Abstract

High carbohydrate as obese diet is not yet available commercially for monkeys. Therefore, this preliminary study was to carry out nutrient intake and digestibility of cynomolgus monkeys (Macaca fascicularis) fed with high soluble carbohydrate diet compared to monkey chow. Five adult female macaques (average body weight 2.67 kg) were made to consume freshly  diet. Commercial monkey chows (contains 3500 cal/g energy and 35% starch) were fed to three adult females (average body weight 3.62 kg). Nutrient intakes and digestibility parameters were measured using modified metabolic cages. Result showed that  average of protein, fat, starch, and energy intakes in treatment diet were higher than control diet (T-test). Fat intake in the treatment diet was three times higher, while starch and energy intakes were almost two times higher than monkey chow. Digestibility percentage of all nutrients were the same in both diets except for the protein. The study concludes that the freshly prepared high sugar diet was palatable and digestible for the cynomolgus monkeys. Further studies are in progress to develop obese diet high in energy content based on fat and source of starch treatments.         Key words: Macaca fascicularis, obese, metabolic cages, intake, digestibility
Fecal Steroid Profile of Female Javan Gibbons (Hylobates moloch) Maintained in Pairing-Typed Cage Hera Maheshwari; Luthfiralda Sjahfirdi; Pudji Astuti; Bambang Purwantara; Hadi Sukardi Alikodra; Dondin Sajuthi; Reviany Widjajakusuma
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.43 KB) | DOI: 10.4308/hjb.17.1.43

Abstract

Estrone conjugate (E1C) and pregnanediol glucuronide (PdG) were predominant steroid metabolites of estrogen and progesterone in feces of most primates and could be used to evaluate ovarian function. These metabolites were determined along with records of genital swelling throughout 3-4 months period from three female Javan Gibbons (Hylobates moloch) maintained in pairing-typed cage at Schmutzer Primate Center, Jakarta (Ullah) and at Taman Margasatwa Taman Sari, Bandung (Donna and Citah). Following methanolic extraction of lyophilized fecal powder, samples were analyzed using enzyme linked immunosorbent assay (ELISA) for E1C and PdG.  In all of the three females observed, both hormone profiles did not indicate any regular cycle of ovarian function even though genital swellings were sometimes observed. In one female (Donna) the hormone patterns showed clear signs of cycle irregularities with extended luteal phase of 40 days and erratic pattern of follicular phase. Of the other two females, no ovarian cycle was found. The data indicate that the fecal steroids analysis is a practical and valuable diagnostic tool for providing reliable information on ovarian function in Javan Gibbon. Factors affected reproductive hormonal profile should be taken in consideration in trying to achieve success in captive breeding program for this species.
Volatile Compounds of Pluchea indica Less and Ocimum basillicum Linn Essential Oiland Potency as Antioxidant PAINI SRI WIDYAWATI; CHRISTOFORA HANNY WIJAYA; PENI SUPRAPTI HARDJOSWORO; DONDIN SAJUTHI
HAYATI Journal of Biosciences Vol. 20 No. 3 (2013): September 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.623 KB) | DOI: 10.4308/hjb.20.3.117

Abstract

This research was conducted to identify volatile compounds of pluchea and basil essential oils and their antioxidant capacity to scavenge a DPPH (1,1-diphenyl-2-picrylhidrazyl) free radical and inhibit lipid peroxidation. Essential oil of pluchea and basil leaves was prepared by hydrodistillation method and then their volatile compounds were identified by GC-MS. The volatile compounds in the essential oil of pluchea leaves consist of 66 components with (10S,11S)-Himachala-3-(12)-4-diene (17.13%) made up the highest proportion of volatile compounds. Basil leaves had 70 volatile components in which the major components were (E)-3,7-dimethyl-2,6-octadienal  (23.98%) and (Z)- 3,7-dimethyl-2,6-octadienal (17.35%). Total phenol levels in pluchea and basil essential oils were 275 and 209 ppm, respectively. DPPH scavenging activity of the essential oil of pluchea leaves was lower than that of basil leaves, conversely inhibition activity of lipid peroxidation in palm oil of pluchea essential oil was higher than that of basil leaves.
Analysis of Intestinal Mucosal Immunoglobulin A in Sprague Dawley Rats Supplemented with Tempeh SUSAN SOKA; ANTONIUS SUWANTO; IMAN RUSMANA; DONDIN SAJUTHI; DIAH ISKANDRIATI; KATHARINA JESSICA
HAYATI Journal of Biosciences Vol. 22 No. 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1507.883 KB) | DOI: 10.4308/hjb.22.1.48

Abstract

Tempeh is a well-known Indonesian fermented food made from soybean. During the fermentation process, microorganisms play an important role in the flavor, texture, and nutritional quality of tempeh. Tempeh has been show to have immuno-modulatory and immune-stimulating properties that may also be caused by the microorganisms in tempeh as they interact between the microbial population in the intestinal tract. The objective of this study was to quantify IgA gene expression at both the transcription and translation levels in Sprague Dawley (SD) rats supplemented with tempeh. A total of 6 female SD rats were divided into 3 groups of 2 rats. The first group was the control and was fed a standard diet without tempeh. The second- and third group were fed with a standard diet supplemented with raw and cooked tempeh, respectively. Ileum tissue samples were collected after tempeh supplementation for 28 days. RNA was extracted from ileum samples, and measurement of IgA gene expression was further analyzed using semi quantitative real-time PCR. The concentration of IgA protein was quantified from ileum lysate using the half sandwich ELISA method. IgA gene expressions in rats supplemented with raw, and with cooked tempeh, were 1.18 and 1.17 fold higher, respectively, compared to the control group. Moreover, IgA protein secretion levels also increased 2.46 and 2.08 fold, respectively, compared to the control group. The result of this study indicates that both raw and cooked tempeh may stimulate IgA secretion, and also that both viable and non-viable microorganisms might stimulate IgA gene expression.
Identification of Diagnostic Mitochondrial DNA Single Nucleotide Polymorphisms Specific to Sumatran Orangutan (Pongo abelii) Populations Puji Rianti; Dyah Perwitasari-Farajallah; Dondin Sajuthi; Joko Pamungkas; Alexander Nater; Michael Krützen
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1662.775 KB) | DOI: 10.4308/hjb.22.4.149

Abstract

The hypervariable region I of mitochondrial DNA has frequently been used to distinguish among populations, in particular in species with strong female philopatry. In such cases, populations are expected to diverge rapidly for hypervariable region I markers because of the smaller effective population size and thus increased genetic drift. This rapid divergence leads to the accumulation of mutations exclusively found in one population, which may serve as diagnostic single nucleotide polymorphisms (SNPs). To date, diagnostic SNPs distinctive to Sumatran orangutan populations have not yet been described. However, given the continuously declining numbers of Sumatran orangutans, this information can be vital for effective conservation measures, especially regarding reintroductions of orangutans in rehabilitation centers. Phylogenetic analyses of 54 samples of Sumatran orangutans from nine sampling sites with good provenance, we found five major clades and a total of 20 haplotypes. We propose a total of 52 diagnostic SNPs that are specific to Sumatran orangutan populations. Data can be used to develop restriction fragment length polymorphism assays to carry out genetic assignments using basic laboratory equipment to assign Sumatran orangutan to their population of origin.
Co-Authors . Permanawati Achmad - Taher Achmad Taher Achmad Taher Achmad, Taher Adi Winarto Agus Harsoyo Agus Harsoyo Agus Lelana Agus Wijaya Ahmad Kamal Nasution Alexander Nater Anita Esfandiari Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Anwar Wardy Warongan Arief Boediono Aryo Tedjo Bambang Purwantara Bayu Febram Prasetyo Bayu Rosadi Budiman Bela C Hanny Wijaya C. Hany Wijaya Cecep Kusmana CHAIRIN N MAROEF Chusnul Choliq Chusnul Choliq Chusnul Choliq Cucu K Sajuthi Dadang Makmun Dadang Makmun Daldiyono Daldiyono Daldiyono Daldiyono Dedi R. Setiadi DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati DIAH ISKANDRIATI DIAH ISKANDRIATI Diah Iskandriati Douglas M. Bowden Dwi Atmoko Agung Nugroho DYAH PERWITASARI -FARAJALLAH Ekowati Handharyani Entang Iskandar Entang Iskandar Entang Iskandar Erdiansyah Rahmi Eric Hayes ERIC HAYES Eric Hayes ERLIN LISTIYANINGSIH Erni Sulistiawati ESTHER ARIFIN Fauzi Abdurrahman Munggaran Fhady Risckhy Loe Fitriya Nur Annisa Dewi Fransiska R Zakaria FUNGKEY HOETAMA Gede Eko Darmono Gregory K. Wilkerson Hadi Sukadi Alikodra Hadi Sukardi Alikodra Hajrawati Hajrawati Harrold Halilintar MR Subu Taopan Henny Nuraini Henny Nuraini HERA MAHESHWARI Huda Darusman Huda S Darusman Huda Salahudin Darusman Huda Shalahuddin Darusman Huda Shalahudin Darusman I Gusti Agung Arta Putra I Gusti Ngurah Sudisma I Ketut Sutama I NENGAH BUDIARSA I Nengah Budiarsa I Nengah Budiarsa I wayan Teguh Wibawan IETJE WIENTARSIH Iis Afriantini Iman Rusmana Iman Supriatna IMELDA WINOTO Indra Bachtiar Irawan Jusuf Irma H Suparto Irma H Suparto Irma H Suparto Irma Herawati Suparto Irma Isnafia Arief Irma Suparto J. Mark Cline Jansen Manansang Jansen Manansang Jayusman Arsiyanti Joesoef Joko Pamungkas JONATHAN STURGIS Kanti Rahmi Fauziyah Kartika Sari KATHARINA JESSICA KEVIN RANDALL PORTER Koeswinarning Sigit Latifah K. Darusman Latifah K. Darusman Leni Maylina Lina Elfita Luluk Lailatul Hasanah Luthfiralda Sjahfirdi LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirdi Luthfiralda Sjahfirji M Agus Setiadi MARYATI SURYA Maxs Urias Ebenhaizar Sanam, Maxs Urias Michael Krützen MOSES AFFANDI Mozes R. Tolihere Muchidin Noordin MUHAMMAD AGIL Nanik Hidayatik Nur Hidayati Nurhidayat . Nurul Oktaviani Oriza Savitri Ariantie Paini Sri Widyati PATRICK J BLAIR Peni Harjosworo PENI SUPRAPTI HARDJOSWORO Permanawati Permanawati Permanawati Permanawati PERMANAWATI PERMANAWATI Pijoh, Deyv Praira, Willy PUDJI ASTUTI Pudji Astuti Pudji Astuti Puji Rianti Puspita Sari R. Harry Soehartono R. Iis Arifiantini R.P Agus Lelana RATNA TAN Retno Wulansari Reviany Widjajakusuma Reviany Widjajakusuma Ria Oktarina RICHARD F GRANT Rini Widayanti Rumlaklak, Yanse Yane Sela S Mariya Sela S. Mariya SELA SEPTIMA MARIYA Sela Septima Mariya Septi Iriani Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Setyo Widi Nugroho Setyo Widodo Silmi Mariya Silmi Mariya Silmi Mariya Silmi Mariya SILMI MARIYA Silvia Triwidyaningtyas Sony Hartono Wijaya Sri Rahmatul Laila Sri Soepraptini Mansjoer Sri Supraptini Mansjoer Srihadi Agungpriyono Suharyanto Suharyanto Suharyanto Suharyanto Sulistiyani . SULISTIYANI SULISTIYANI SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani Sulistiyani, Sulistiyani Suparto, Irma Sus Derthi Widhyai Sus Derthi Widhyari SUSAN SOKA SUSANA WIDJAJA Suzy Tomongo TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Ari Prabowo Teguh Budipitojo Tejasari . Thomas Mata Hine Thomas Mata Hine Tiara Widyaputri Tri Wahyu Pangestiningsih Trini i Susmiat Trini Susmiati Tuti Suryati Tuti Suryati Tuty L Yusuf Tuty Laswardi Yusuf Tuty Laswardy Yusuf Unang Supratman Uus Saefullah Uus Saepuloh Uus Saepuloh Uus Saepuloh Uus Saepuloh UUS SAEPULOH UUS SAEPULOH Vetnizah Juniantito Villiandra Villiandra Villiandra Villiandra Villiandra Villiandra Vincentia Trisna Yoelinda Wijaya, Agus Willy Praira Yanse Yanne Rumlaklak Yanti . Yanti Yanti Yasmina A Pramastri Yasmina A. Paramastri Yasmina Arditi Pramastri YASMINA PARAMASTRI Yoga Yuniadi Yohana Tri Hastuti Yohana Tri Hastuti Yohana Tri Hastuti Yuli Purwandari Kristiangingrum Yundari, Yundari Zulfitra Utami Putri Zulfitra Utami Putri Zuraida Zuraida