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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Ali Husni
Balai Besar Bioteknologi dan Sumberdaya Genetika Pertanian Jl. Tentara Pelajar No. 3A, Bogor 16111, Indonesia
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Radiosensitivitas dan Seleksi Mutan Putatif Jeruk Keprok Garut (Citrus reticulata L.) berdasarkan Penanda Morfologi Karyanti ,; Agus Purwito; Ali Husni
Indonesian Journal of Agronomy Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.529 KB) | DOI: 10.24831/jai.v43i2.10417

Abstract

ABSTRACTMandarin Citrus cv. garut is a of local citrus variety with several superiority, but not competitive to imported citrus. Quality improvement have been the subject of citrus breeding programme. The objective of this research was to determine lethal dose 50 (LD50) and putative mutans regenerant selected based on morphological markers. Callus was irradiated at doses of 0, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Gy and regenerated through somatic embryogenesis. The result of radiosensitivity showed that lethal dose 50 (LD50) analyzed by Curve Expert 1.4 software was 75.31 Gy. Observation on callus showed variation on morphology and weight of callus. Callus growth was not inhibited at doses 0-50 Gy but at doses 60-100 Gy callus growth was inhibited. Gamma irradiation also affected the formations of somatic embryos. After six weeks on maturation medium, the highest number of embryo somatic was produced from doses of 20 and 100 Gy and following culture on germination medium, the highest number of planlet was produced from doses of 20 and 40 Gy. Based on morphological characters, the selected regenerants had variability of 0-58%.Keywords: gamma irradiation, germination, lethal dosis 50 (LD50), maturation, somatic embryo
Co-Authors Agus Purwito Karyanti ,