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RESISTANCE EVALUATION ON POPULATIONS OF CROSSES BETWEEN TRANSGENIC POTATO KATAHDIN RB AND NON-TRANSGENIC ATLANTIC AND GRANOLA TO LATE BLIGHT (Phytophthora infestans) IN CONFINED FIELD TRIAL Ambarwati, Alberta Dinar; Herman, Muhammad; Purwito, Agus; Sumaraw, Sientje Mandang; Aswidinnoor, Hajrial
Indonesian Journal of Agricultural Science Vol 12, No 1 (2011): April 2011
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Late blight resistance gene (RB gene) isolated from Solanum bulbocastanum, is a broad resistance gene against all races of Phytophthora infestans. The gene was transformed into Katah-din event SP904 and SP951 using Agrobacterium tumefaciens and these transgenic plants have been crossed with susceptible potato cultivars Atlantic and Granola. Populations of the crosses have been molecularly characterized for the integration of the RB transgene. The study aimed to evaluate the resistance of the populations of crosses between transgenic Katahdin RB  and susceptible non-transgenic parents (Atlantic and Granola) to late blight in a confined field trial at Pasir Sarongge, Cianjur, West Java. A total of 84 clones originated from four popula-tions were evaluated for resistance to late blight. These included 22 clones of Atlantic x transgenic Katahdin SP904, 16 clones of Atlantic x transgenic Katahdin SP951, 19 clones of Granola x transgenic Katahdin SP904, and 27 clones of Granola x transgenic Katahdin SP951. Observations of the late blight infection were conducted when late blight symptoms were detected, i.e. at 56, 60, 63, 70, and 77 days after planting (DAP). The result showed there were high variations in the resistance level of all the 84 clones tested. Clones of crosses between susceptible parents (Atlantic or Granola) and resistant parents (transgenic Katahdin SP904 or Katahdin SP951) showed a similar pattern based on the area under disease progress curve (AUDPC) value, i.e. 377.2 greater than the AUDPC of the resistant parents (180.1), but smaller than that of the susceptible parents (670.7). Observation at 77 DAP resulted four resistant potato clones having resistance score of 7.0-7.6, higher than the transgenic parents Katahdin SP904 (4.6) and Katahdin SP951 (6.8), i.e. clone B8 (Atlantic x transgenic Katahdin SP951) with resistance score of 7.6 and clones B26 (Atlantic x transgenic Katahdin SP951), C183 (Granola x transgenic Katahdin SP904), and D89 (Granola x transgenic Katahdin SP951) with resistance score of 7. These four transgenic potato resistant clones need to be further developed as promising potato clones to late blight.
DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica) Ibrahim, Meynarti Sari Dewi; Hartati, Rr. Sri; Rubiyo, Rubiyo; Purwito, Agus; Sudarsono, Sudarsono
Indonesian Journal of Agricultural Science Vol 14, No 2 (2013): October 2013
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.
Efficacy of RB gene in transgenic potato Katahdin SP904 and SP951 to West Java isolates of Phytophthora infestans Ambarwati, A. Dinar; Sumaraw, S. M.; Purwito, Agus; Herman, M.; Suryaningsih, E.; Aswidinnoor, Hajrial
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
Publisher : Jurnal AgroBiogen

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Potato late blight, caused by Phytophthora infestans is one of the most devastating plant disease. Potato yield losses due to this disease ranged from 47-100%. A major late blight resistance gene, called RB, previously was identified in the wild potato species Solanum bulbocastanum. RB gene has been integrated into cultivated potato Katahdin using Agrobacterium-mediated transfor-mation, and showed durable and broad spectrum resistance either in laboratory assay or in confined field trial. Evaluation of transgenic Katahdin SP904 and SP951 was conducted to verify whether the RB gene with broad spectrum to all known races of P. infestans in the United States and in Toluca, Mexico was also effective against P. infestans isolates in Indonesia. Efficacy of RB gene was evaluated for foliar and tuber resistance to West Java isolates. Transgenic Katahdin were more resistant in foliar than non transgenic plants, at 14 days after inoculation. Diseases intensity of transgenic Katahdin SP904 and SP951 were 19.8-43.8%, whereas non transgenic Katahdin, Granola, and Atlantic were 46.9-100%. In contrast to the foliar resistance phenotype, RB-containing tubers in transgenic Katahdin did not exhibit increased resistance to Lembang, Pangalengan and Galunggung isolates. Tubers of transgenic Katahdin SP904, SP951, and non transgenic Katahdin showed lesion volume of 0.93, 0.91, and 0.91 cm3, respectively. RB gene in transgenic Katahdin showed efficacy against late blight P. infestans in foliar, but did not showed efficacy in tuber. Transgenic Katahdin RB thus providing a potential source of resistance for breeding programs.
ANALYSIS OF COMBINING ABILITY, HETEROSIS EFFECT AND HERITABILITY ESTIMATE OF YIELD-RELATED CHARACTERS IN SHALLOT (Alium cepa var. ascalonicum Baker) Farid, Noor; Sugiharto, Arifin Noor; Herison, Catur; Purwito, Agus; Sutjahjo, Surjono Hadi
AGRIVITA, Journal of Agricultural Science Vol 34, No 1 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Low productivity of shallot in Indonesia can be improved through development of high yielding variety. The availability of genetic information related to the character being improved is inevitable for effective breeding program. In this study, seven shallot genotypes were evaluated for their combining ability for yield improvement using half diallel cross.  Heterosis effect and heritability estimate was also investigated for yield-related characters.  The results showed that there were significant differences in general combining ability (GCA) and specific combining ability (SCA) among the evaluated genotypes. Tiron and Timor had the greatest GCA.  The greatest SCA and heterosis value was found in crosses of Kuning/Tiron, Timor/Bima Juna, Tiron/Timor and Kuning/Sibolangit. Heterosis effect varied from low to high. Broad sense heritability estimate for all characters was high, but narrow sense heritability was low for most charaters.  The dominant gene action observed on all yield-related characters suggests that the evaluated genotypes are potential to be used in breeding for high yielding hybrid varieties.   Keywords: GCA, SCA, heterosis, heritability, shallot
Induksi Kalus Embriogenik dan Daya Regenerasi Kopi Arabika Menggunakan 2,4- Dichlorophenoxyacetic Acid dan 6-Benzyladenine Ibrahim, Meynarti Sari Dewi; Hartati, Rr Sri; Rubiyo, Rubiyo; Purwito, Agus; Sudarsono, Sudarsono
Jurnal Tanaman Industri dan Penyegar Vol 4, No 2 (2013): Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Embriogenesis somatik kopi Arabika (Coffea arabica L.) masih mengalami kendala dalam meregenerasikan planlet dari eksplan yang dikulturkan. Kemampuan eksplan daun membentuk embrio dalam proses embriogenesis somatik kopi sangat dipengaruhi oleh komposisi media dan zat pengatur tumbuh. Penelitian bertujuan untuk melihat pengaruh pemberian 2,4-Dichlorophenoxyacetic Acid dan 6-Benzyladenine dalam proses pembentukan kalus embriogenik dan daya regenerasi kopi Arabika. Penelitian dilakukan di Unit Pengembangan Benih Unggul Pertanian, Badan Penelitian dan Pengembangan Pertanian pada bulan Juli 2011 sampai Desember 2012. Bahan tanaman yang digunakan adalah daun kopi Arabika varietas S795 koleksi Balai Penelitian Tanaman Industri dan Penyegar. Rancangan perlakuan menggunakan rancangan acak lengkap dengan 6 ulangan, masing-masing ulangan terdiri dari 5 eksplan. Induksi kalus menggunakan 5 kombinasi perlakuan 2,4-D 1 mg/l + BA 0 mg/l; 2,4-D 1 mg/l + BA 1 mg/l; 2,4-D 1 mg/l + BA 2 mg/l; 2,4-D 2 mg/l + BA 1 mg/l; dan kontrol (tanpa penambahan 2,4-D dan BA). Peubah yang diamati meliputi jumlah kalus, persentasi kalus embriogenik, berat basah kalus, dan jumlah proembrio. Hasil penelitian menunjukkan perlakuan 2,4-D 1 mg/l + BA 0 mg/l; 2,4-D 1 mg/l + BA 1 mg/l; 2,4-D 1 mg/l + BA 2 mg/l; dan 2,4-D 2 mg/l + BA 1 mg/l dapat membentuk kalus kecuali perlakuan kontrol. Berat kalus, persentasi kalus embriogenik, dan jumlah proembrio tertinggi diperoleh pada media kombinasi 2,4-D 2 mg/l dan BA 1 mg/l. Kalus yang mampu beregenerasi berasal dari media kombinasi 2,4-D 1 mg/l dan BA 2 mg/l dengan persentasi 16,67% dengan 6 kecambah per 0,2 gram kalus.Kata Kunci: Coffea arabica, 2,4-Dichlorophenoxyacetic Acid, 6-Benzyladenine, embriogenesis somatikRegeneration of planlets from cultured explants has been an obstacle in somatic embryogenesis of arabica coffee (Coffea arabica L.). The ability of leaf explants to generate embryos in somatic embryogenesis process of coffee was affected by composition of media and plant growth regulators. The objectives of the research was to examine the effect of 2,4-Dichlorophenoxyacetic Acid dan 6-Benzyladenine in the process of embryogenic callus and regeneration potential of arabica coffee. The study was conducted at Agricultural Superior Seed Development Unit, Indonesian Agency for Agricultural Research and Development (IAARD) from July 2011 to December 2012. Plant material used was leaves of S795 variety which is collected by Indonesian Industrial and Beverage Crops Research Institute. The research was arranged in completely randomized design with 6 replications, each replication consist of 5 explants. Callus induction used 5 treatments, i.e. 2,4-D 1 mg/l + BA 0 mg/l; 2,4-D 1 mg/l + BA 1 mg/l; 2,4-D 1 mg/l + BA 2 mg/l; 2,4-D 2 mg/l + BA 1 mg/l; and control (without 2,4-D and BA). Variables observed were number of callus, percentage of embryogenic callus, callus fresh weight and number of proembryo. Result showed that all treatments can produce the callus except control. Combination of 2,4-D 2 mg/l and BA 1 mg/l gave the highest of fresh weight of callus, percentage of embryogenic callus, and number of proembryo. Regenerating callus of 16.67% with the number of sprouts of 6 per 0.2 gram only derived from combination of 2,4-D 1 mg/l BA and 2 mg/l. 
Kajian Pendahuluan: Perpindahan Gen dari Tanaman Kentang Transgenik Katahdin RB ke Tanaman Kentang Non Transgenik Ambarwati, A. Dinar; Herman, M.; Purwito, Agus rifcb@indo.net.id; Sofiari, Eri; Aswidinnoor, hajrial
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3114

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ABSTRACTPreliminary study: Gene transfer from transgenic potato Katahdin RB to non transgenicpotato. One of the concerns associated with the release of transgenic crops, is the possibilityof the gene flow from transgenic crops to neighboring crops of the same species or to relatedspecies. In plants, gene flow is a routine process occur through the natural hybridization. Theopportunity for gene flow occur depends principally on two factors, the degree of sexualcompatibility between donor and recipient species, and the physical distance between thetwo. The experiment was conducted to determine whether the gene flow from transgenicpotato Katahdin RB to non transgenic was occurred, based on selection using a 50 mg/lkanamycin, and to estimate gene flow mediated by natural hybridization at different isolationdistances. Preliminary result indicated that a rapid and simple method using MS0 liquid mediawith kanamycin 50 mg/l was effective for screening the seeds. There was a gene flow fromtransgenic potato Katahdin RB to non transgenic, based on a rapid and simple selectionmethod using 50 mg/l of kanamycin as selectable marker. The isolation distance used in thestudy were 0.8, 1.6, 2.4, 3.2, 4.0, 4.8, 5.6, 6.4, 7.2, 8.0, 8.8, 9.6, 10.4, and 11.2 m from the row oftransgenic potato Katahdin RB. The gene flow through natural hybridization at a isolationdistances of (0.8 - 1.6 m), (2.4 – 4 m), and (4.8 – 6.4 m) from transgenic to non transgenic plantswere 13.78, 10.92, and 3.82%, respectively. At a distance of 7.2 – 8 m, the frequency of gene flowwas declined to 0%. The frequency of gene flow from transgenic potatoes to non transgenicpotatoes markedly decreased by increasing the isolation distance, and was negligible at 7.2 m.Key words : natural hybridization, transgenic potato RB, kanamycin selection
INDUKSI MUTASI DAN SELEKSI IN VITRO TANAMAN GANDUM (Triticum aestivum L.) Sari, Laela; Purwito, Agus; Soepandi, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 2 (2016): December 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.087 KB) | DOI: 10.29122/jbbi.v3i2.36

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INDUCTION MUTATION AND SELECTION OF IN VITRO PLANT OF WHEAT (Triticum aestivum L.)The goal of this research was to produce wheat crop which is tolerant to lowland condition.Six varieties were used, Dewata, Selayar, Alibey, Oasis, Rabe and HP1744. This research consisted of 4 stages: production of the best callus on MS medium containing 3 g/L 2.4-D, induced mutation of embryogenic callus using EMS, in vitro selection of callus at temperature of 27–35°C, and callus regeneration. The best result for callus production was 76% for Dewata and 70% for Selayar varieties. Higher concentration of EMS and longer soaking time decreased the percentage of callus growth. LC50 for Dewata was 0.3% EMS at 30 minutes and that for Selayar was 0.1% EMS at 60 minutes. The higher the temperature was, the lower was the adaptation tolerant of the plants, and callus growth was inhibited. At the highest temperature (35°C) the callus did not grow at all.Keywords: Induced mutation, Triticum aestivum, EMS, in vitro selection, callusABSTRAKTujuan penelitian ini adalah untuk merakit tanaman gandum yang toleran pada dataran rendah. Varietas yang digunakan ada 6 yaitu Dewata, Selayar, Alibey, Oasis, Rabe dan HP-1744. Penelitian terdiri atas empat tahap yaitu induksi pembentukan kalus terbaik menggunakan media MS + 3 g/L 2,4-D (dipilih dua varietas yang terbaik), induksi mutasi kalus embriogenik menggunakan EMS, seleksi kalus in vitro pada suhu 27–35°C, dan regenerasi. Hasil induksi kalus terbaik terdapat pada varietas Dewata sebesar 76% dan Selayar sebesar 70%. Semakin tinggi konsentrasi EMS dan semakin lama waktu perendaman yang digunakan maka semakin menurun persentase pertumbuhan kalus. LC50 varietas Dewata adalah EMS 0,3% waktu 30 menit sedangkan LC50 varietas Selayar adalah EMS 0,1% waktu 60 menit.Semakin tinggi suhunya maka semakin berkurang toleran adaptasi tanaman tersebut, dan pertumbuhan kalus semakin sedikit. Bahkan pada suhu tertinggi yaitu suhu 35°C tidak ada pertumbuhan kalus sama sekali.Kata Kunci: Induksi mutasi, Triticum aestivum, EMS, seleksi in vitro, kalus
Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi Sari, Laela; Purwito, Agus; Sopandie, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6612

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The purpose of this study was to identify the selection criteria to obtain a superior mutant derived from the wheat plants of such varieties as Dewata, Selayar and Alibey, adaptive in medium land. The analysis of agronomic growth characters showed a significantly effect on a growth percentage of the initial growth (8 mutants), flowering time (1 mutant), panicle stem length (15 mutants), number of panicles (7 mutants), the number of grains per panicle (8 mutants), grain weight observed (8 mutants), grain weight per genotype (6 mutants), leaf area (2 mutants) and leaf greenness (5 mutants). The effects on the characters of ripe time, harvest, panicle length and plant height were not significant. The mutants of Dewata, Selayar and Alibey could be selected based on the characters of panicle stem length, number of grains per panicle and grain weight per observation because these characters generated more mutants than the other characters. The correlation analysis between the characters of growth and yield components of wheat mutants showed that the number of grains per panicle was positively correlated with the grain weight observed, while the length of panicle stem was positively correlated with grain weight per genotype, number of panicles and leaf area. Hopefully some mutants produced could adapt to the tropical medium land, thus adding to the diversity of wheat germplasm in Indonesia, thereby reducing the import of wheat to Indonesia.How to CiteSari, L., Purwito, A., Sopandie, D., Purnamaningsih, R. & Sudarmonowati, E. (2016). Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi. Biosaintifika: Journal of Biology & Biology Education, 8(3), 353-361.
In Vitro Induction Of Tetraploid Pummelo ’Nambangan’ (Citrus maxima (Burm.) Merr.) By Colchicine Treatment Using Germinated Seed, Shoot Tip And Cotyledonary Node As Explants Wulandari, Dyah Retno; Purwito, Agus; Susanto, Slamet; Husni, Ali; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i1.105

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Tetraploid citrus are important for interploidal hybridization to create triploid seedless citrus.  Colchicine is the most commonly used as antimitotic agent to induce polyploid plants.  Tetraploid induction by colchicine in Pummelo ‘Nambangan’ was conducted in vitro using different types of explants.  The aim of this research was to induce tetraploid pummelo ‘Nambangan’ by colchicine treatment using germinated seed, shoot tip and cotyledonary node as explants.  Tetraploid shoot induction was conducted by soaking germinated seeds, shoot tips and cotyledonary nodes in 0.1% colchicine for 1, 3 and 5 h.  Regenerant shoots were grown on MS medium and their growth was observed after four weeks in culture.  Ploidy level was determined using flow cytometry analysis.  Stomata density, length and width of stomatal guard cell were also recorded. The results showed that shoot elongation was inhibited by colchicine treatment.  Soaking of shoot tip explants in 0.1% colchicine for 1 h resulted in 66.66% of putative tetraploid shoots.  Compared to diploid shoots, tetraploids had lower stomata density but bigger in guard cell size. Keywords: colchicine, tetraploid, pummelo (Citrus maxima (Burm.) Merr.), flow cytometry, stomata,
MULTIPLIKASI IN VITRO ANGGREK HITAM (Coelogyne pandurata Lindl.) PADA PERLAKUAN KOMBINASI NAA DAN BAP Kartiman, Roni; Sukma, Dewi; Aisyah, Syarifah Iis; Purwito, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1006.134 KB) | DOI: 10.29122/jbbi.v5i1.2908

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In Vitro Multiplication of  Black Orchid (Coelogyne pandurata Lindl.) Using the Combination of NAA and BAPABSTRACTBlack orchid is an indigenous plant from Kalimantan, Indonesia. It becomes endangered because of forest over-exploitation and its low natural reproduction rate. Tissue culture is considered to offer a solution to conserve and propagate this species. The aim of this research was to evaluate the effect of Naphtalene Acetic Acid (NAA) and 6-Benzile Amino Purine (BAP) on shoots multiplication of black orchid. The basic medium used was a half of Murashige & Skoog (MS) composition supplemented with 150 mLL-1 coconut water. Initial explants used were 6-month-old shoots of germinating seeds. The shoot cultures were incubated for 23 weeks. Results showed that the best combination for shoot multiplication was NAA 0.0 mgL-1 with BAP 0.2 mgL-1. Shoot grew better on medium with BAP and without NAA while roots growth was better on medium without the two plant growth regulators. The addition of BAP up to 0.3 mgL-1 increased the leaf number, which however decreased at higher BAP concentration.Keywords: BAP, black orchid, Coelogyne pandurata, multiplication, NAA ABSTRAKAnggrek hitam merupakan flora langka asli Kalimantan, Indonesia. Keberadaa anggrek ini di alam semakin langka akibat eksploitasi berlebihan dan sulitnya perbanyakan secara alami. Kultur jaringan merupakan metode untuk mengatasi kelangkaan anggrek ini. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi NAA dan BAP terhadap multiplikasi anggrek hitam. Media dasar yang digunakan adalah ½ MS dengan penambahan air kelapa 150 mLL-1. Eksplan yang digunakan adalah tunas hasil semai biji umur 6 bulan. Kultur tunas diinkubasi selama 23 minggu. Hasil penelitian menunjukkan bahwa kombinasi terbaik untuk multiplikasi tunas adalah NAA 0 mgL-1 dengan BAP 0,2 mgL-1. Tunas tumbuh lebih baik dalam media dengan penambahan BAP tanpa NAA, sedangkan akar pada media tanpa NAA dan BAP. Penambahan BAP sampai 0.3 mgL-1 mampu meningkatkan jumlah daun, namun menurun dengan penambahan di atas konsentrasi tersebut.Kata Kunci: anggrek hitam, BAP, Coelogyne pandurata, multiplikasi, NAA
Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Anas D Susila Andri Ernawati Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atika Fathur Rahmi Atra Romeida Awang Maharijaya Bambang S. Purwoko Budi Marwoto Budi Marwoto Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Catur Herison dan Budi Winarto dan Sudarsono Dewi Citra Sari Dewi Sukma Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Iis Rahmawati Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Julius D. Nugroho Kartiman, Roni Kartiman, Roni Karyanti , Kikin H Mutaqin Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Marlin MASKROMO, ISMAIL MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi MUHAMMAD HERMAN Muhammad Herman Muhammad Mahmud MUHAMMAD SYUKUR Ni Made Armini Wiendi Nidya Ravenska Noor Farid Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie NURUL KHUMAIDA Nurwita Dewi Prima Muklisa Purba, Dumaris Priskila RAGAPADMI PURNAMANINGSIH Rd. Selvy Handayani Rd. Zainal Frihadian Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah ROEDHY POERWANTO Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati RR. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw Sari, Laela SIENTJE MANDANG SUMARAW Sientje Mandang Sumaraw SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO SUDIRMAN YAHYA Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syarifah Iis Aisyah Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Willy Bayuardi Suwarno Wulandari, Dyah Retno Yopi Kurniawan