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Alveolar Bone Density after the Orthodontic Treatment in Patients of Rsgm Universitas Airlangga Ernie Maduratna Setiawatie; Mohamad Rubianto; Muhammad Naufal Ilmi Roswand
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14719

Background: There is a relation between the orthodontic therapy and the periodontal tissue. In the movingteeth and planning treatment, the periodontal tissue is very influential in an orthodontic therapy. During themovement of the teeth, all components of the periodontal tissue such as the bones, periodontal ligaments,and gingiva will change and move together. Some studies indicate a decrease in alveolar bone fraction andalveolar bone density. The changes in density are seen by comparing the new bone mineralization withthe old bones. Purpose: This research aims to analyze the differences in alveolar bone density after anorthodontic treatment in patients of RSGM Universitas Airlangga. Methods: This study used a patient’spanoramic radiograph at the time before the orthodontic treatment and the time after it. Photographs ofpanoramic radiographs were measured using a digital densitometer. Results: The results of the studyrevealed a decrease in alveolar bone density after orthodontic treatment, both anterior and posterior. Theaverage decrease in the anterior was 35.4%, while in the posterior was a decrease of 31.5%. Conclusion:There was a decrease in the alveolar bone density after orthodontic treatment in the anterior and posteriorsections.

MULTIPLE GINGIVAL RECESSION COVERAGE TREATED WITH VISTA TECHNIQUE USING ACELLULAR DERMAL MATRIX (ADM) COMBINED WITH PLATELET RICH FIBRIN (PRF): A CASE REPORT Rosa Pratiwi; Ernie Maduratna Setiawatie
ODONTO : Dental Journal Vol 6, No 1 (2019): July 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.6.0.56-61

Background: Gingival recession is an exposure of the root surface due to migration of the marginal periodontal tissues apical to the cemento enamel junction. There are so many root coverage procedures, among these techniques, VISTA technique combined with ADM and PRF offers minimally invasive approach.Case Management: A-40-year-old male visited Periodontics Clinic of DentalHospital Airlangga University with the chief complaint of poor aesthetic resulting from exposed root surfaces in upper right front region of the jaw. Root coverage by VISTA technique using ADM combined with PRF was planned to this case.Discussion: The minimal invasive VISTA technique approach, combined with ADMand PRF has a number of advantages for successful treatment of multiple recession defects. VISTA technique was used because it can maintain better blood supply and maintain critical papillary integrity. ADM works like an autogenous graft by providing a bioactive matrix consisting of collagen, elastin, blood vessel channels, and bioactive protein that support natural revascularization, cell repopulation, and tissue remodeling. Growth factors present in PRF plays a crucial role in hard and soft tissue repair.Conclusion: VISTA technique together with ADM and PRF membrane can besuccessfully used as a treatment method for multiple gingival recessions of Miller’s class I defects

Crude toxin of Aggregatibacter actinomycetemcomitans serotype-B increase PARP-1 expression in gingival epithelium Ernie Maduratna Setiawatie
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 1 (2012): March 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitants) serotype-B has long been associated with aggressive periodontitis. Gingival epithelial cell is exquisitely sensitive to the toxin so that may lead to disruption of the epithelial protective barrier, facilitating invasion and perturbation of the underlying connective tissue. Currently suggested that Aa serotypeB produce protein toxin that caused DNA strand breaks. PARP-1 is an abundant nuclear protein functioning as a DNA nick-sensor enzyme. PARP-1 was one of the first identified substrates of caspases, the main executioners of apoptosis. Therefore, a role for PARP-1 in the regulation of apoptosis has been suggested. Purpose: The purpose of this study was to prove PARP-1 expression in gingival epithelium caused by toxin exposure of A. actinomycetemcomitant serotype-B. Methods: This is an experimental study involving twenty adult mice strain Swiss Webster (balb C) divided randomly into two groups: control group (Group A) and toxin group (Group B). Both group were acclimated for one week before treatment. Group A was applied topically with sterile distillated water every 12 hours. Group B was applied topically by 100μg/ml of crude toxin A. actinomycetemcomitant serotype B at the buccal area of mandibular anterior teeth using Hamilton syringe. The mice were sacrificed at 24 hours after toxin application, and then the tissue sections of gingival epithelium were stained with immunohistochemistry to reveal the PARP-1 expression. The data were analyzed with t-test. Results: The PARP-1 expression exhibited an increase with the toxin group (mean= 48.9; SD= 2.01) compared with the control group (mean= 25.21; SD= 1.72). DNA fragmentation appeared from the agarose gel examination, marked as DNA laddering, indicate the cell apoptosis. Conclusion: In conclusion the crude toxin exposure of A. actinomycetemcomitant serotype-B leads to DNA fragmentation and increase PARP-1 expression.Latar belakang: Aggregatibacteractinomycetemcomitans (A. actinomycetemcomitant) serotype-B merupakan etiologi utama periodontitis agresif. Sel epitel gingiva sangat sensitif terhadap toksin sehingga dapat mengganggu epitel sebagai pertahanan awal gingiva, membantu invasi toksin dan mengganggu jaringan ikat dibawahnya. Saat ini diketahui bahwa toksin bakteri Aa serotype-B menyebabkan putusnya rantai DNA. PARP-1 merupakan protein dalam intisel yang berfungsi sebagai DNA nicksensor enzyme. PARP-1 merupakan penanda awal apoptosis, sehingga peran PARP-1 dalam pengaturan apoptosis perlu diteliti Tujuan: Tujuan dari penelitian ini adalah untuk meneliti ekspresi PARP-1 pada epitel gingiva yang dipapar toksin bakteri A. actinomycetemcomitant serotype-B. Metode: Penelitian eksperimen pada 20 mencit strain Swiss Webster (balb C) dibagi secara random dalam 2 kelompok, kelompok kontrol (Group A) dan kelompok perlakuan (Group B). Kedua kelompok diaklimasi sebelumnya selama 1 minggu. Kelompok diaplikasi secara topikal dengan air destilasi steril setiap 12 jam. Kelompok B diaplikasi 100 μg/ml toksin A. actinomycetemcomitant serotype-B secara topikal dengan menggunakan Hamilton syringe. Mencit dimatikan 24 jam setelah aplikasi toksin kemudian potongan epitel gingiva dilakukan pemeriksaan secara imunohistokimia untuk melihat ekspresi PARP-1. Data dianalisis dengan uji-t. Hasil: Ekspresi PARP-1 menunjukkan penigkatan pada kelompok perlakuan (Mean = 48,9; SD = 2,01) bila dibanding kelopok kontrol (Mean= 25,21; SD= 1,72). Tampak adanya gambaran DNA fragmentasi pada pemeriksaan gel elektroforesis yang menunjukkan adanya apoptosis. Kesimpulan: Dapat disimpulkan bahwa paparan toksin A. actinomycetemcomitant serotype-B menyebabkan DNA fragmentasi, dan meningkatkan ekspresi PARP-1.

Volatile sulphur compounds elimination: A new insight in periodontal treatment Ernie Maduratna Setiawatie; Rikko Hudyono
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 4 (2011): December 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Recent evidences had demonstrated a link between halitosis and apoptosis in periodontitis. Periodontal pathogenic micro-organisms produce volatile sulphur compounds (VSCs). VSCs are toxic to periodontal tissue. Purpose: The purpose of this paper was to reveal the mechanism of VSCs in periodontal breakdown according to the most recent knowledges. Reviews: Halitosis is mainly attributed to VSCs such as hydrogen sulfide, methyl mercaptan and dimethyl sulfide. Several studies demonstrated a strong relationship between VSCs and periodontal disease progression. VSCs are released from amino acid breakdown from food, protein, cells, blood and saliva. In prone subjects, the VSCs may cause alteration in tissue integrity by increasing its permeability and facilitate the endotoxin to penetrate the tissue barrier. They may also causing apoptotic in gingival and periodontal tissue, which are considered the main pathogenesis in aggravating the periodontitis. VSCs may also initiate the increase of proinflammatory cytokines which is considered to have negative effects in host response. Conclusion: VSCs had been shown to have detrimental effects in gingival and periodontal ligament cells. The use of chlorine dioxine agent and topical antioxidant is beneficial in controlling the periodontal disease severity.Latar belakang: Penelitian terakhir menunjukkan adanya hubungan antara halitosis dengan terjadinya apoptosis pada periodontitis. Mikroorganisme penyebab periodontitis memproduksi volatile sulphur compounds (VSCs) yang bersifat toksik terhadap jaringan periodontal. Tujuan: Tujuan penulisan ini adalah membahas mekanisme VSCs dalam menyebabkan kerusakan periodontal berdasarkan penelitian terakhir yang ada. Tinjauan pustaka: Halitosis seringkali dikaitkan dengan timbulnya VSCs seperti hidrogen sulfida, metil merkaptan, dan dimetil sulfida. Penelitian terakhir menunjukkan bahwa VSCs yang dilepaskan dari pemecahan asam amino makanan ternyata memiliki korelasi dengan kerusakan jaringan periodontal. Pada subjek yang peka, VSCs dapat menyebabkan terjadinya disintegritas epitel dengan meningkatkan permeabilitasnya sehingga endotoksin dapat masuk melewati epitel. Adanya VSCs tersebut memicu terjadinya apoptosis pada jaringan gingiva dan ligamen periodontal, dimana proses ini menyebabkan keparahan penyakit periodontal. VSCs juga dapat mencetuskan peningkatan sitokin proinflamasi yang dapat menyebabkan kerusakan jaringan periodontal. Kesimpulan: VSCs dapat menyebabkan kerusakan pada gingiva dan ligamen periodontal. Penggunaan bahan chlorine dioxine untuk dan antioksidan topikal sangat berguna dalam mengontrol keparahan penyakit periodontal.

Hydroxyapatite combined with hyaluronic acid metronidazole gel increased the quantity of osteoblasts in the alveolar bone wistar rat Ernie Maduratna Setiawatie
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 4 (2015): December 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Bone graft material have been used extensively in bone healing and periodontal treatment. Alloplast such as hydroxyapatite are frequently used to repair and reconstruct bone defects. By merely applying hydroxyapatite for the treatment of bone is not fully effective yet to produce new bone regeneration. Locally applied high molecular hyaluronic acid (HA) has been shown to stimulate differentiation and migration of mesenchymal cells. Recent studies on regenerative surgical procedures indicate that reduction of bacterial burden at the wound site may improve the clinical outcome of regenerative therapy. Metronidazole has the greatest bacteriostatic effect. A clinical application of HA metronidazole gels during the surgical therapy may reduce the bacterial contamination of surgical wound site. Purpose: The purpose of this study was to examine the effect of combination hydroxyapatite and HA metronidazole gel 1% on osteoblast cell number after wound healing process in the wistar rats incisor tooth extraction socket. Method: Twenty seven wistar rats were divided randomly into 3 groups. The first group consisted of wistar rats given hydroxyapatite were subjected to the mandibular incisor extraction socket. The second group were given hydroxyapatite combined with HA metronidazole gel 1%. The control group were filled with blood. Wistar rats were euthanized on day 14 and then preparation for histological examination was stained using hematoxylin-eosin and then the numbers of the osteoblasts were calculated. Result: The differences in each group were tested by one way Anova test (α=0.05). The numbers of osteoblasts in each group had a significant difference (p<0.05): the highest numbers of osteoblasts were found in the group that was given hydroxyapatite combined with HA metronidazole, followed by the lower numbers of osteoblasts in the hydroxyapa ite group and the lowest numbers of osteoblasts were found in the control group. Conclusion: Adjunctive application of HA metronidazole to hydroxyapatite after tooth extraction increase the number of osteoblast in the treatment of bone defects on a wistar rat model.

Inhibition Activity Of Black Cumin Toothpaste Contain With Detergent Compared With Black Cumin Extract Non Detergent To The Growth Of Supragingiva Plaque Bacteria Resgita Nadila Masya; Noer Ulfah; Maria Elisea Kiswantoro Hadinoto; Bambang Hadi Sugito; Badai Septa Wahyudadi; Ernie Maduratna Setiawatie
Indonesian Journal of Dental Medicine Vol. 2 No. 2 (2019): July - December
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v2i2.2019.41-44

Background: Plaque can inhibit the antibacterial activity of salivary function, which can damage the periodontal tissue. Several preventive measures can be done to control plaque, mechanically or chemically. Mechanical plaque control in combination with chemical plaqie control such as toothpaste can reduce plaque. The role of SLS in toothpaste which can facilitate plaque detachment from the tooth surface. Herbal toothpaste has been developed using herbal ingredients that have antibacterial properties as black cumin (Nigella sativa). The contents of black cumin, thymoquinone, thymol, and tannins, have been proven to be effective in inhibiting the growth of plaque bacteria. Purpose: This study aims to identify the inhibitory ability of black cumin toothpaste extract containing detergent compared to black cumin extract toothpaste without detergent on the growth of supragingival plaque bacteria. Methods: This research method of diffusion of wells on BHIB media was conducted in four research groups namely negative control, cumin paste with SLS, non SLS cumin paste, and SLS and carried out bacterial planting on MHA media. Next measure the diameter of the inhibition zone around the well. Results: There was a significant difference in each group against the inhibitory growth of supragingival plaque bacteria on the Oneway ANOVA test results (p <0.05) Conclusion: Black cumin extract toothpaste containing 2% of detergent has better inhibition against supragingival plaque bacteria than non cumin detergent 3% black cumin toothpaste.

Number of Osteoblasts and Osteoclasts in Combination Carbonate Hydroxyapatite, Platelet Rich Fibrin (PRF) and Antioxidant in Socket Rats Wistar After Tooth Extraction Rachma Widarena; Hengky Marlie; M. Rubianto; Ernie Maduratna Setiawatie
Indonesian Journal of Dental Medicine Vol. 4 No. 1 (2021): January - June
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v4i1.2021.15-18

Background: Bone graft materials have been used extensively to support bone healing after tooth extraction. Bone healing could be increased reactive oxygen species (ROS) that prolong the phase of the inflammatory and delay reparative phase. Antioxidants are substances that can improve and reduce the number and the damage produced by ROS. Hence, the utilization of antioxidant which is utilized in conjunction to Carbonate hydroxyapatite is expected to increase the success of bone healing. Purpose: The aim of this study is to compare the number of osteoblasts and osteoclast in the process of bone healing after employing Carbonate Hydroxyapatite and antioxidant in the Wistar rats’ incisor tooth extraction socket. Methods: Twenty-seven male Rattus norvegicus strain Wistar rats were divided into 3 treatment groups. This study uses a post-test only control design. Sample of 27 rats were divided into 3 groups. Mandibular incisive is extraction. Group 1, socket is lefted to fill with blood (control). Group 2, socket is filled bonegraft and antioxidants and group III, socket is filled antioxidants, bonegraft and platelet rich fibrin (PRF). After that, the wound is sutured. On day 14, the mice are terminated then viewed in microscopy of osteoblasts. The preparation of the bone tissues was given the staining by hematoxylin-eosin and then the numbers of the osteoblasts and osteoclast were calculated. Results: Statistical testing by using one-way ANOVA has proved that there are significant differences in the number of osteoblasts in all 3 groups (p = 0.000). The highest numbers of osteoblasts were found in the group that was given Carbonate Hydroxyapatite combined with antioxidant and PRF and the lowest numbers of osteoblasts were found in the control group. Conclusion: Combination bonegraft, antioxidants and PRF could be increased the highest number of osteoblasts and could be decreased the lowest number of osteoclasts compared with control group and the group bonegraft and antioxidants.

Obat kumur Nigella sativa sebagai terapi alternatif pada gingivitis Westy Agrawanty; Ernie Maduratna Setiawatie
Makassar Dental Journal Vol. 3 No. 3 (2014): Vol 3 No 3 Juni 2014
Publisher : Makassar Dental Journal PDGI Makassar

Gingivitis merupakan suatu penyakit inflamasi di gingiva yang mempengaruhi jaringan periodonsium, tetapi tidak menyebabkan kehilangan perlekatan jaringan. Etiologi utamanya adalah bakteri plak sehingga tujuan utama terapi gingivitis adalah untuk mengurangi atau menghilangkan bakteri. Pada kasus ini, obat kumur dari ekstrak biji Nigella sativa digunakan sebagai terapi tambahan setelah perawatan mekanis (scaling dan root planing). Nigella sativa memiliki aktivitas antioksidan dan antimikroba. Seorang pasien wanita berumur 45 tahun dengan keluhan utama nyeri pada gusi depan, sering berdarah jika menyikat gigi dan ada pembengkakan khususnya di gusi depan rahang atas. Pasien tidak memiliki riwayat alergi, penyakit sistemik, dan riwayat penyakit dalam keluarga yang berkaitan. Dari pemeriksaan klinis, tampak gingiva anterior kemerahan dan terdapat inflamasi terutama di daerah margin gingiva rahang atas, dengan GI skor 2 dan BOP grade 2, dengan poket gingiva 4-5 mm. Setelah 4 minggu penggunaan obat kumur dua kali sehari, gingivitis hilang. Tekstur permukaan dan warna gingiva kembali normal, dan kedalaman poket hilang. Disimpulkan bahwa aktivitas antioksidan dan antimikroba obat kumur Nigella sativa efektif menghilangkan gingivitis.

MULTIPLE GINGIVAL RECESSION COVERAGE TREATED WITH VISTA TECHNIQUE USING ACELLULAR DERMAL MATRIX (ADM) COMBINED WITH PLATELET RICH FIBRIN (PRF): A CASE REPORT Rosa Pratiwi; Ernie Maduratna Setiawatie
Odonto : Dental Journal Vol 6, No 1 (2019): July 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Gingival recession is an exposure of the root surface due to migration of the marginal periodontal tissues apical to the cemento enamel junction. There are so many root coverage procedures, among these techniques, VISTA technique combined with ADM and PRF offers minimally invasive approach.Case Management: A-40-year-old male visited Periodontics Clinic of DentalHospital Airlangga University with the chief complaint of poor aesthetic resulting from exposed root surfaces in upper right front region of the jaw. Root coverage by VISTA technique using ADM combined with PRF was planned to this case.Discussion: The minimal invasive VISTA technique approach, combined with ADMand PRF has a number of advantages for successful treatment of multiple recession defects. VISTA technique was used because it can maintain better blood supply and maintain critical papillary integrity. ADM works like an autogenous graft by providing a bioactive matrix consisting of collagen, elastin, blood vessel channels, and bioactive protein that support natural revascularization, cell repopulation, and tissue remodeling. Growth factors present in PRF plays a crucial role in hard and soft tissue repair.Conclusion: VISTA technique together with ADM and PRF membrane can besuccessfully used as a treatment method for multiple gingival recessions of Miller’s class I defects

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