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Iswari Saraswati Dewi

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian Jl. Tentara Pelajar 3A, Bogor 16111, Indonesia

Author-ID : 2434440

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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1

Respon Galur-galur Harapan Padi Sawah Dihaploid terhadap Salinitas pada Fase Bibit Anggita Duhita Anindyajati; Bambang Sapta Purwoko; Iswari Saraswati Dewi; Willy Bayuardi Suwarno
Indonesian Journal of Agronomy Vol. 49 No. 3 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Due to climate change, Indonesian rice breeders have recently experienced salt issues. Salinity has been reported in many studies to cause plant growth reduction and severe yield loss in rice. This research aimed at evaluating salinity tolerance of doubled-haploid (DH) lines derived from anther culture at the seedling stage. The research was conducted at ICABIOGRAD’s greenhouse using a split-plot randomized complete block design. The main plot was NaCl concentration (0 mM and 120 mM) and the subplot was rice genotypes consisting of 14 doubled-haploid (DH) lines, four new released varieties (NRV), one tolerant check (Pokkali), and one sensitive check (Inpara 4). Seedlings of each rice genotype were grown hydroponically supplemented with different levels of NaCl. Plant growth and visual injury according to Standard Evaluation System for Rice from IRRI were assessed 14 days after NaCl addition. The results showed that 120 mM NaCl reduced shoot height, root length, leaf number, tiller number, shoot and root fresh weight, shoot and root dry weight, total fresh and dry weight biomass. The evaluation based on the seedling’s visual injury concluded that 11 DH lines and 2 NRV were tolerant, and 3 DH lines and 2 NRV were moderately tolerant. Keywords: anther culture, evaluation, hydroponically, tolerance

Karakterisasi Fisikokimia Beras Galur-galur Padi Hitam Dihaploid Muhammad Jauhar Firdaus; Bambang Sapta Purwoko; Iswari Saraswati Dewi; Willy Bayuardi Suwarno
Indonesian Journal of Agronomy Vol. 50 No. 1 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The quality of grain and rice which includes physical and chemical quality (physicochemical) is a consumer preference that needs to be considered. The character of the quality of grain and rice includes appearance, texture and taste. The aim of the study was to measure or identify quantitatively the physicochemical character of the dihaploid rice lines of black rice. The experiment was conducted at the Quality Laboratory, Muara Experimental Station, Indonesian Center for Rice Research (BB Padi) from August to September 2021. The study used a completely randomized design with 10 replicates or samples per genotype. The genotypes used consisted of 16 (14 dihaploid lines of black rice and two check varieties, namely Aek Sibundong and Jeliteng). Observations of physical quality included water content, broken husk rice, head rice, milled rice, rice shape, length of rice and grains of rice lime. The chemical characters of rice observed were amylose content, gelatinization temperature and organoleptic. The results showed that the AW6 line had a close similarity with the check variety shown in the cluster analysis in Group 1. The distinguishing traits in Group 1 were 13.6% moisture content, 88.7% head rice, 76% broken rice, 70% milled rice, score alkaline 1-4, medium to high gelatinization temperature, lean shape, savory taste and small liming. The AW6 line was included in Group 1 because it had similar characteristics to the check varieties. Keywords: amylose, black rice, physicochemical

Evaluasi Galur-galur Padi Sawah Dihaploid Hasil Kultur Antera Menggunakan Seleksi Indeks Cucu Gunarsih; Bambang Sapta Purwoko; Iswari Saraswati Dewi; Willy Bayuardi Suwarno; Nafisah
Indonesian Journal of Agronomy Vol. 50 No. 1 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Pemulia tanaman melakukan seleksi multi-karakter dengan metode yang sesuai, salah satunya adalah indeks seleksi. Analisis multivariat dapat digunakan dalam menyusun model indeks seleksi. Percobaan ini bertujuan untuk menyeleksi galur-galur dihaploid dengan potensi hasil yang tinggi dan memiliki penampilan agronomi baik berdasarkan indeks seleksi. Percobaan dilakukan pada bulan Agustus 2018 sampai dengan September 2019 menggunakan rancangan acak lengkap dengan tiga ulangan dan satu faktor yaitu genotipe. Genotipe yang diuji ialah 89 galur dihaploid padi sawah hasil kultur antera ditambah 4 varietas pembanding (Ciherang, Inpari 30 Ciherang Sub1, Inpari 41, dan Inpari 35 Agritan). Unit percobaan adalah satu bibit per pot. Model indeks seleksi disusun dengan mempertimbangkan hasil analisis ragam, analisis korelasi, analisis lintas, analisis komponen utama, dan ideotipe tanaman yang dituju. Hasil studi ini mengungkapkan bahwa terdapat keragaman sifat agronomi yang diuji dari galur-galur dihaploid. Model seleksi indeks yang disusun adalah sebagai berikut: I (nilai indeks) = 3*0.75 hasil + 0.85 jumlah gabah total per malai – 0.85 tinggi tanaman. Berdasarkan model tersebut, diperoleh 85 galur dengan nilai indeks dan produksinya lebih tinggi dibandingkan Inpari 41. Galur-galur dihaploid terpilih diikutsertakan pada pengujian berikutnya di lapangan. Kata kunci: heritabilitas, korelasi, sidik lintas, variabilitas

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