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Pengecatan Imunohistokimia HER2 Menggunakan Susu Skim dan Normal Serum Arya Iswara; Sri Sinto Dewi; Yulfa Ariza Masruro
Biomedika Vol 10 No 1 (2017): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

HER2 (Human Epidermal Growth Factor Receptor 2) merupakan suatu reseptor pada permukaan sel yang berpengaruh pada proliferasi jaringan, mutasinya dapat menjadi onkogen. Over ekspresi dari HER2 pada kasus kanker dapat dilihat dengan teknik imunohistokimia (IHC). Protein blocking merupakan salah satu langkah dalam pengecatan IHC yang berfungsi menghalangi ikatan non spesifik pada jaringan dengan menggunakan normal serum dan protein solution (susu skim). Tujuan penelitian mengetahui gambaran hasil pengecatan IHC menggunakan normal serum dan susu skim. Penelitian secara eksperimental dengan pendekatan cross sectional. Sampel penelitian jaringan kanker payudara HER2 positif dengan stadium +2 dari satu organ dan pasien yang sama. Pengecatan IHC menggunakan teknik Strep (Avidin) Biotin Complex. Pengecatan menggunakan normal serum didapatkan hasil +2, menggunakan susu skim 1% didapatkan hasil +3, sedangkan menggunakan susu skim 2% dan 3% didapatkan hasil +2. Terdapat perbedaan yang signifikan antara normal serum dengan susu skim 1%. Tidak terdapat perbedaan yang signifikan antara normal serum dengan susu skim 2% dan susu skim 3%. Simpulan adalah normal serum dapat diganti dengan susu skim 2%.

Rasio Penutupan Luka pada Tikus Diabetes Diinduksi Streptozotocin dengan Perlakuan Dressing Tipe Pasif dan Interaktif (Penelitian Pendahuluan) Eko Naning Sofyanita; Arya Iswara
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v3i2.8045

Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol.

POLA SENSITIVITAS Eschericia coli TERHADAP ANTIBIOTIK METRONIDAZOLE Arya Iswara
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Escherichia coli (E. coli) have a potency to be a pathogen bacteria cause an illness. Antibiotic treatments to a patient have a purpose to eliminate the pathogen bacteria. Bacteria resistance to antibiotic was influenced by the intensity of antibiotic treatment in a region, the uncontrolled antibiotics treatments would increase the antibiotic resistance of bacteria. This research was using four different sample, they were feces from diarrhea, ice block, waters from well, and ketchup. Approximately 1 gram from each sample diluted in 10 mL of BHI and then incubated for 6 hours in 37 C. After 6 hours incubations, the samples inoculated in EMBA and incubated in 37 C for 24 hours. The metallic green colored bacteria colony inoculated in Mac Conkey Agar and incubated in 370C for 24 hours. The separated colony sub cultured into HIA and tested using biochemistry test. Sensitivity test conducted using the Kirby Bauer method. The test result showed that 5 samples were sensitive to metronidazole and 26 samples wereresistant to metronidazole.Key word: E.coli, metronidazole, sensitivity.

EFEKTIVITAS INFUSA KULIT JERUK PURUT (Citrus hystrix DC.) TERHADAP PERTUMBUHAN Candida albicans PENYEBAB SARIAWAN SECARA in vitro Zakiyatul Khafidhoh; Sri Sinto Dewi; Arya Iswara
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Candida albicans is a normal flora in the oral mucosa, tongue, and palate, but it could become the pathogenic one. If the amounts were excess so that it could cause thrush. Kaffir lime peel infusion contains saponins, tannins, flavonoids, coumarin which are active antifungal compound. The purpose of this study was to examine the effectiveness of kaffir lime peel infusion to the growth of Candida albicans which causes thrush with a consentration of 10%, 15%, 20% and contact time of 5 minutes, 10 minutes, 15 minutes. Method of this study used an experimental research laboratory. Suspension Candida albicans 100 mL with dilution 10-4 of McFarland 0,5 and contacted to the kaffir lime peel infusion 10%, 15%, 20% with three repetitions. After 5 minutes, 10 minutes, and 15 minutes put 100 μL and then inoculated into SGA antibiotics then incubated at 37˚C for 48 hours. Based on the results of this study concluded that kaffir lime peel infusion can inhibit the growth of Candida albicans. The best results was the 20% consentration and contact time of 15 minutes with an average colonies of 3×104 CFU/100 μL. The higher consentration of kaffir lime peel infusion the more able to inhibit the growth of Candida albicans and the longer the contact time kaffir lime peel infusion, the more can inhibit the growth of Candida albicans.Keywords: Infusion, Citrus hystrix DC., Candida albicans.

EFEK PEMBERIAN Morinda citrifolia,L PADA KADAR TGF-Β SERUM DAN EKSPRESI KOLAGEN PADA GINJAL TIKUS DIABETES NEFROPATI Arya Iswara; Udadi Sadana; Indranila Kustarini
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2014: PROSIDING SEMINAR NASIONAL HASIL - HASIL PENELITIAN & PENGABDIAN
Publisher : Universitas Muhammadiyah Semarang

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Background: Morinda citrifolia L contain components that lowering the oxidative stress andthe ability to regenerate the renal function through the measurement of renal extra cellularmatrix and growth factor. The detection from renal functions remission can be measuredthrough the transforming growth factor beta (TGF-β) level, and collagen deposition. Aims: This experiment was to prove the Morinda citrifolia L effect to lowering the TGF-β level andcollagen deposition in streptozotocin-induced nephropathy diabetic Sprague Dawley rats.Method: The post test only control group design experiment in thirty rats randomized intopositive control group and four treatment group and induced with streptozotocin in 40mg/kgWBdoses. The rats treated with Morinda citrifolia extract which was divided into four doses(10;20;40;80mg/dL) then determine TGF-β level and collagen deposition. The data analyzedusing SPSS ver 17 with oneway-ANOVA and post hoc LSD, Kruskal-Wallis and Mann-WhitneyU and Spearman’s Rho with confidence interval 95% (p<0,05). Result: The statisticallyanalysis from rats treated with morinda extract resulted a significant result in TGF-β level, correlation between TGF-β level and collagen deposition (p<0,05). Conclusion: Morindacitrifolia treatment can substantially improve the activity of the renal function, shown by lowerTGF-β level and better density of collagen deposition compared with control group and 10-20mg/dL morinda treatment dose showed the best result.Keywords: Nephropathy diabetics, Morinda citrifolia, TGF-β level and collagen deposition.

VARIASI KONSENTRASI KOH DAN WAKTU CLEARING TERHADAP KUALITAS PREPARAT AWETAN Pediculus humanus capitis Arya Iswara; Fitri Nuroini
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Pembuatan sediaan permanen entomologi/insekta diawali dengan perendaman dalam KOH, dilanjutkan dengan proses dehidrasi, proses clearing dan proses mounting. Perendaman dalam KOH bertujuan menipiskan lapisan kitin pembentuk eksoskeleton pada insekta. Tahapan Clearing merupakan salah satu tahapan pembuatan awetan permanen yang bertujuan menjadikanstruktur parasit insekta terlihat lebih jelas, jernih, dan transparan. Penelitian dilakukan dengan variasi perendaman dalam larutan KOH 5%, 10%, 15%, dan 20%. selama 24 jam, serta variasi lama waktu clearing 5, 15, 25, dan 60 menit. Pengamatan sediaan awetan permanen dilakukan dengan menilai kualitas sediaan awetan permanen. Kualitas sediaan awetan permanen meliputi kejernihan, kualitas warna, dan keutuhan sediaan awetan permanen. Hasil penelitian menunjukkan hasil yang bervariasi, kualitas yang buruk didapatkan pada kombinasi antara variabel waktu clearing 5 menit pada seluruh variasi konsentrasi KOH dan pada variabel waktu clearing 15menit pada konsentrasi KOH 5%. Hasil dengan kualitas baik ditunjukkan pada variabel waktu clearing 15 menit pada konsentrasi KOH 10%, 15%, dan 20%, serta pada variabel vaktu clearing 25 dan 60 menit pada seluruh variasi konsentrasi KOH. Hasil kualitas yang baik disertai dengan peningkatan dan penurunan nilai skoring. Hasil dari penelitian ini dapat ditarik kesimpulan bahwa semakin lama waktu clearing akan menghasilkan preparat yang lebih baik dengan nilai skoring yang berbeda berdasarkan persentase KOH yang digunakan.Keywords: KOH, clearing, Pediculus humanus capitis

Pengaruh Variasi Waktu Clearing Terhadap Kualitas Sediaan Awetan Permanen Ctenocephalides Felis Arya Iswara
Jurnal Labora Medika Vol 1, No 1 (2017): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Sediaan awetan permanen adalah teknik pengawetan preparat untuk berbagai macam parasit, salah satunya adalah Ctenocephalides felis. Proses pembuatan preparat awetan melalui tahapan clearing. Clearing merupakan proses perendaman didalam larutan xylol selama 15 menit yang bertujuan menjadikan struktur Ctenocephalides felis terlihat jernih. Hasil penjernihan akan menghasilkan hasil yang maksimal apabila digunakan waktu perendaman dalam xylol selama semalam. Hasil perendaman semalam dapat memperlihatkan struktur tubuh Ctenocephalides felis yang lebih jelas, jernih dan transparan. Lamanya waktu penjernihan yang diperlukan dirasa kurang efektif sehingga dirasa untuk dilakukan penelitian penggunakan waktu yang lebih pendek, penelitian ini bertujuan untuk mengetahui pengaruh variasi waktu clearing terhadap kualitas sediaan awetan permanen Ctenocephalides felis. Penelitian ini merupakan penelitian eksperimen. Sampel yang didapatkan diproses untuk dilakukan pembuatan sediaan permanen dengan menggunakan 3 variasi waktu clearing yaitu 5, 15 dan 25 menit.Hasil penelitian menunjukkan kualitas sediaan dengan perlakuan clearing 25 menit lebih baik dibandingkan kualitas sedian dengan perlakuan clearing 15 menit dan 5 menit. Penelitian ini membuktikan bahwa semakin lama dilakukan proses clearing maka semakin baik kualitas sediaan awetan permanen yang didapatkan.

Minyak Zaitun Sebagai Pengganti Xylene pada Prosesing Jaringan Histologis untuk Pewarnaan Kulit dan Hepar Mencit dengan Hematoxylin Eosin: Sebuah Studi Perbandingan Eko Naning Sofyanita; Arya Iswara; Djoko Priyatno
Jaringan Laboratorium Medis Vol 4, No 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8688

Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. Xylene is the most commonly used aromatic solvent for clearing agents and deparaffinizing agents in histopathology laboratories, but xylene is one of the most dangerous chemicals found in histology laboratories. Based on the dangers posed by xylene, a replacement material is needed. Several xylene substitutes such as reagents derived from limonene, aliphatic hydrocarbons, vegetable oils and mineral oils have been commercially developed, but the available xylene substitutes are still less effective, more expensive, and still as dangerous as xylene itself. Substitutes that are considered safer are from natural oils, one of which is olive oil which has similarities with xylene, namely in hydrocarbon and phenol compounds. This study used two types of tissue, namely skin and liver from mice (Mus musculus) which were cut into two parts; the first part uses xylene as and the other part uses olive oil as a clearing agent. The assessment of hematoxylin eosin staining results were determined based on the category of assessment of cell structure, such as the cell nucleus, cytoplasm, and color uniformity carried out by three readers and five fields of view for each microscopic slide at 40X magnification of the objective lens. Comparison of observations of the xylene group in skin and liver tissue 100% got a good score on the cell nucleus, cytoplasm, and color uniformity. (p=1,000). The olive oil group had a slight difference in color uniformity in liver tissue when compared to skin, but not statistically different (p=0.773). The comparison of the overall readings of the xylene and olive oil groups on skin and liver tissue also showed no statistically different (p=0.262). So it can be concluded that olive oil can be recommended as a substitute for xylene in the clearing process in histological tissue processing.

Perbandingan Kualitas Hasil Pewarnaan MenggunakanHematoxylinEosin danEkstrak Daun Jati Sebagai Pengganti Eosin Muhamad Jumardi; Arya Iswara; Gela Setya Ayu Putri; Tulus Ariyadi
Prosiding Seminar Nasional Unimus Vol 6 (2023): Membangun Tatanan Sosial di Era Revolusi Industri 4.0 dalam Menunjang Pencapaian Susta
Publisher : Universitas Muhammadiyah Semarang

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Penggunaan eosin bersifat karsinogenik apabila digunakan dalam jangka panjang secara terus menerus.Efek negatif dari penggunaan dapat menyebabkan kanker dan sisa limbah dapat merusak lingkungan.Diperlukan alternatif zat warna untuk mengurangi dampak penggunaan eosin salah satunya dengan pewarnaalami, salah satu contoh pewarna alami adalah daun jati muda. Daun jati muda memiliki kandungansenyawa pigmen antosianin yang memberikan warna merah yang memiliki kemampuan sebagaiantioksidan sehingga berpotensi untuk digunakan sebagai pewarna alami hematoxylin-eosin. Tujuan untukmengetahui perbandingan kualitas hasil pewarnaan menggunakan hematoxylin-eosin dan pewarna alami.Penelitian secara eksperimental menggunakan ekstrak daun jati. Sampel yang diteliti sebanyak 24 preparatjaringan kulit dengan 4 kelompok perlakuan yaitu, 6 preparat untuk pewarnaan Hematoxylin-Eosin, 6preparat untuk pewarnaan ekstrak daun jati 2:1 (gr/mL), 6 preparat untuk pewarnaan ekstrak daun jati 1:1(gr/mL), 6 preparat untuk pewarnaan ekstrak 1:2 (gr/mL). Hasil kualitas pewarnaan yang diwarnai denganekstrak daun jati 2:1 didapatkan kualitas kurang baik sedangkan 1:1 dan 1:2 didapatkan kualitas baik denganpresentase 100%. Uji statistik dengan Kruskal-Wallis-Test diperoleh hasil (p value <0,05) sehingga terdapatperbedaan yang signifikan terhadap penggunaan Hematoksilin-Eosin dan ekstrak daun jati 1:1 dan 1:2.Ekstrak daun jati dapat digunakan sebagai pewarna dalam pewarnaan Hematoxylin-Eosin. Kata Kunci : Daun Jati 2:1,1:1,1:2, Hematoxylin Eosin, Preparat Kulit.

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