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Okky Setyawati Dharmaputra

Institut Pertanian Bogor SEAMEO BIOTROP

Author-ID : 2452259

Medicine & Pharmacology

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Serangan Tribolium castaneum pada Beras di Penyimpanan dan Pengaruhnya terhadap Serangan Cendawan dan Susut Bobot Okky Setyawati Dharmaputra; Hariyadi Halid; Sunjaya Sunjaya
Jurnal Fitopatologi Indonesia Vol 10 No 4 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Tribolium castaneum is an important insect pest of stored milled rice in Indonesia. The effect of T. castaneum on fungal infection of stored milled rice was investigated together with moisture content and weight loss. Milled rice were placed in glass jars (250 g/jar) and infested with 5, 10, and 20 pairs of T. castaneum. As control, the jars contained only milled rice. Three replications were made for each treatment (including the control). The jars were stored in storage room for 1, 2, and 3 months. Moisture contents (based on wet weight) of milled rice were determined using the oven method. Fungi were isolated and enumerated using dilution plating, followed by pour plate method on Dichloran 18% Glycerol Agar (DG18). Weight loss (based on dry weight) was determined after 3 months of storage.The results showed, that the population of adult T. castaneum increased with the increase in the number of pairs of infested T. castaneum and storage duration. The moisture contents of milled rice were relatively constant during storage. It was assumed, that the moisture contents were more influenced by the environmental conditions rather than the occurrence of T. castaneum. Nineteen fungal species were isolated Aspergillus flavus and Penicillium citrinum were the dominant fungal species. Total fungal population decreased after 2 and 3 months of storage, with the increase in the number of pairs of infested T. castaneum. Weight loss increased with the increase in the number of pairs of infested T. castaneum. Based on weight loss of milled rice, the best storage duration was found when the number of pairs of T. castaneum was five pairs, the duration of storage was 1, 2 or 3 months of storage.

Mikobiota pada Buah Cabai untuk Pengendalian Hayati Colletotrichum capsici Okky Setyawati Dharmaputra; Lisdar Idwan Sudirman; Melly Fitriani
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Colletotrichum capsici is a pathogenic fungus causing anthracnose on various tropical fruits, especially chilli. Biological control agents have been used as an alternative method to control postharvest diseases. This study aims to examine the antagonistic potential of mycobiota on red chilli fruit against C. capsici. The pathogen was obtained from diseased red chilli fruits collected from three traditional markets in Municipality of Bogor, isolated on potato dextrose agar (PDA) medium containing chloramphenicol (100 mg L-1). Candidates of antagonistic mycobiota were isolated from healthy chilli fruits using serial diution method, followed by pour-plate method on PDA medium containing chloramphenicol (100 mg L-1). C. capsici isolate BIO51046 showed highest pathogenicity on chilli fruit var. IPB Perbani compared to other isolates. Seven isolates of filamentous fungi and 7 yeast isolates were obtained from healthy chilli fruits. Test of antagonism using dual culture method obtained 3 filamentous fungal isolates (Plectosphaerella cucumerina, MF2 and Aspergillus flavus) and 1 yeast isolate (Issatchenkia orientalis) which inhibited the growth of C. capsici BIO 51046 more than 70%. Plectosphaerella cucumerina and I.orientalis did not cause any diseases on chilli fruits var. IPB Perbani. Therefore, these 2 isolates were considered as potential antagonist against C. capsici BIO51046 as the causal agent of anthracnose of chilli

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