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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Prosiding Seminar Biologi JSDH BERITA BIOLOGI Jurnal Penelitian Sains Jurnal Penelitian Pertanian Tanaman Pangan Menara Perkebunan Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik)

LISDAR I. SUDIRMAN

Bagian Mikologi, Departemen Biologi, Institut Pertanian Bogor

Author-ID : 624776

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Peran Makhluk Tersembunyi dan Terabaikan bagi Kesehatan dan Lingkungan I Sudirman, Lisdar
Prosiding Seminar Biologi 2015: Seminar Nasional Mikrobiologi Kesehatan dan Lingkungan
Publisher : Prosiding Seminar Biologi

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Cendawan yang termasuk dalam fungal kingdom adalah organisme unik dan bukan termasuk dalam dunia tumbuhan. Cendawan dapat bersifat mikroskopis dan makroskopis (makrofungi, jamur) yang dapat dilihat oleh mata telanjang. Cendawan mempunyai kemampuan berasosiasi dengan organisme lain seperti dengan ganggang dan atau sianobakteri sehingga terbentuk organisme lain yang dinamakan liken. Makrofungi dan liken mempunyai peran menguntungkan bagi kesehatan dan lingkungan. Daerah tropis, termasuk Indonesia kaya akan keragamanÂ ke dua organisme tersebut tetapi kurang dieksplorasi dan diteliti sehingga pemanfaatannya jauh tertinggal dari negara di dunia, bahkan dengan negara di Asean. Pada makalah ini akan dipaparkan potensi makrofungi atau jamur terutama jamur pelapuk kayu putih (white rot fungi) dan liken dalam bidang kesehatan dan lingkungan dalam mengatasi polutan.Kata Kunci: cendawan, liken, makrofungi

PATOGENISITAS CENDAWAN Lecanicillium sp. PTN01 TERHADAP PENGGEREK TONGKOL JAGUNG Helicoverpa armigera (HUBNER) (LEPIDOPTERA: NOCTUIDAE) Ginting, Sempurna; Santoso, Teguh; Munara, Yayi; Anwar, Ruly; Sudirman, Lisdar
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Helicoverpa armigera is one of the most important agricultural pests because it has a high reproductive rate and resulted in economic losses. One of the H. armigera control techniques that is in accord with IPM principle is the utilizing of entomopathogenic fungus. The aim of this research was to test the pathogenicity of Lecanicillium (Lecanicillium sp. PTN01) against H. armigera. Pathogenicity test was conducted by conidia application of Lecanicillium sp. PTN01 on both the larvae and eggs at density of 105, 106, 107 conidia/ml. The control group was only treated with steril water. Polymerase chain reaction (PCR) was performed for molecular identification Lecanicillium sp. PTN01. The results showed that Lecanicillium sp. PTN01 able to inhibit egg hatching 13.75%, and cause mortality of first instar larval survival 98.75%. The result of virulence test to the highest larval mortality at 107 conidia/ml density was 41,25%, with the values of LT25, 50, 75 were 3.95, 7.12, 12.82 (days) and LC25, 50, 75 were 4.6 x 105, 1.7 x 106, 4.6 x 109 (conidia/ml). The DNA sequence analysis of ITS 1 and ITS 4 primers showed that Lecanicillium sp. PTN01 was similar to fungus species L. kalimantanense strain BTCC F23 with 94% homology.

Keragaman Genetik berbagai Isolat Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) dan Virulensinya terhadap Crocidolomia pavonana Trizelia, Trizelia; Santoso, Teguh; Sosromarsono, Soemartono; Rauf, Aunu; Sudirman, Lisdar I
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The objectives of the research were to study the genetic variability of various Beauveria bassiana (Bals.) Vuill. isolates andvirulence to Crocidolomia pavonana. Thirteen isolates of B. bassiana were isolated from Nilaparvata lugens (Stål),Leptocorisa oratorius (F.), Thrips sp., Hypothenemus hampei (Ferr.), Crocidolomia pavonana (F.), Spodoptera litura (F.),Chrysodeixis chalcites (Esp.), and Riptortus linearis (L.) pests from different geographical locations in Indonesia. Fourteendifferent random primers were used to amplify DNA. Clustering of isolates were obtained using Unweight Pair GroupMethod Avarage (UPGMA). The results showed that only OPA08 primer generated more polymorphic DNA fragments.Result of clustering analysis showed that B. bassiana isolates forms two groups having genetic similarity equal to 55%.Clustering of isolates does not correlate to host type nor geographic origin. Mortality of second instar C. pavonana larvaewas dependent on the fungal isolates. At a concentration of 108 conidia/ml, isolate Bb-La2 had the highest virulence whichcaused 65% mortality of 2nd instar larvae. Isolates of B. bassiana is virulent against C. pavonana larvae present in the samegenotype group.

Karakterisasi Î±-Amilase dari Aspergillus versicolor 3a1 yang Diproduksi pada Media Limbah Cair Tapioka Aini, Fitratul; Mubarik, Nisa Rachmania; Manaf, Lisdar A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

The aim of this experiment was to characterize A. versicolor 3a1 Î±-amylase produced on cassava liquid waste media. Two types of media, base and combination media, were used as a comparison. Cassava liquid waste media contains 1% cassava starch, 1% yeast extract, 0.13% KH2PO4, and 0.05% MgSO4 diluted in cassava liquid waste. Base media contains same composition but using aquadest as a solvent, and combination media using mixture of aquadest and cassava liquid waste. A. versicolor 3a1 Î±-amylase showed its maximum specific activity in cassava liquid waste, base, and combination media after 3, 7, and 4 days incubation, respectively. Crude extract of Î±-amylase fromA. versicolor 3a1 was precipitated in 20-80% (w/v) ammonium sulphate. Precipitation of A. versicolor 3a1 Î±-amylase with 70% (w/v) ammonium sulphate on cassava liquidwaste, 60% on base media, and 60% on combination media will increase its specific activity 16.6, 4.28, and 5.65 times, respectively, compared to the specific activities ofcrude before precipitation. Î±-Amylase crude extract from A. versicolor 3a1 from all media showed its highest specific activity at 70oC and pH 5.0, and addition of FeSO4 increased the specific activity. Precipitated A. versicolor 3a1 Î±-amylase from all media showed its highest specific activity at 70oC and pH 6.0. Addition of FeSO4 precipitated 3a1 Î±-amylase from base and combination media will increase its specific activity, while MgSO4 will increase its specific activity in cassava liquid waste media. Thermostability assay revealed that the crude and the precipitated 3a1 Î±-amylase were relatively stable at 70oC up to 180 minutes incubation, except for precipitated3a1 ï¡-amylase on cassava waste media. Crude Î±-amylase 3a1 was relatively stable at pH 5-9 up to 1 hour incubation with wide pH ranges, while the precipitated with narrow pH ranges.

Peningkatan Efikasi Cendawan Lecanicillium lecanii untuk Mengendalikan Telur Hama Kepik Coklat pada Kedelai Prayogo, Yusmani; Santoso, Teguh; Kartosuwondo, Utomo; Sudirman, Lisdar I.
Jurnal Penelitian Pertanian Tanaman Pangan Vol 30, No 1 (2011): April 2011
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Lecanicillium lecanii is considered as an effective entomopathogenic fungi to control eggs of brown stink bug on soybean. Its efficacy is indicated by reducing the level of hatching of brown stink bug eggs. One factor affecting the efficacy of the entomopathogen in the field is its intolerant to sunlight exposure. A research was conducted in the screen house of the Indonesian Legumes and Tuber Crops Research Institute (ILETRI), Malang, from March to August 2008. The study was aimed at determining the effect of addition of vegetable oils on the efficacy of lecanii to control stink bug eggs on soybean. The trial was arranged in a randomized complete design with two factors. The first factor consisted of three vegetable oils i.e, peanut oil, soybean oil, and coconut oil. The second factor consisted of four oil concentration i.e; 2 ml/l, 5 ml/l, 10 ml/l, and without oil (control). Result showed that addition of vegetable oils as adjuvant increased significantly the growth and development of the entomopathogen, as indicated by the increase of colony diameter and number of conidia of lecanii in vitro. The fungal conidia persisted on the soybean plants until seven days after the adjuvant application at the rate of 10 ml/l water, while the conidia persisted only for one day on plants which was not applied with the vegetable oils. Hatchability of the brown stink bug eggs was only 20% when peanut oil was used as an adjuvant. Not all hatched eggs of the brown stink bug developed into adult, hence, the number of adult bugs that cause damages on soybean pods were reduced. In general, the addition of vegetable oil increased efficacy of the entomopathogen up to 40%. Among the vegetable oils tested, peanut oil was the most effective to increase the efficacy of lecanii to control brown stink bug egg on soybean.

Effect of Leaf Litters and Soils on Viability of Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill LISDAR IDWAN SUDIRMAN; YUSMANI PRAYOGO; YUNIMAR YUNIMAR; SEMPURNA GINTING
HAYATI Journal of Biosciences Vol. 15 No. 3 (2008): September 2008
Publisher : Bogor Agricultural University, Indonesia

Viability of Beauveria bassiana is extremely low due to toxic compounds in soils. This research was aimed to study the effect of four groups of media on viability of B. bassiana Bb-Pb2. The first group was leaf litters of onion, flowering white cabbage, cabbage, and chinese mustard, respectively; the second group was the soils containing decomposed residues of each plant of the first group; the third group was the mixtures of each media of both groups above (1:1), and the fourth group was natural top soil as a control. Each plastic bag filled with one kg of each medium was inoculated with ten ml of B. bassiana conidia (106/ml of concentration) and incubated in open area for 8 weeks. The results showed that all leaf litters of those plants and their compost soils affected the fungal viability. The highest decreasing number of colony was found on onionâ€™s leaf litters, soil containing of decomposed onion, and the mixtures of both media. The treated B. bassiana showed significant reducing abilities of growth, conidia production and conidia germination on PDA media, except the one of control. It is suggested that the Bb-Pb2 isolate might not be effective as bioinsecticide in the soils containing either those leaf litters or composts. Key words: Beauveria bassiana, viability, leaf litters, soils

Characterization Antimicrobes of Pliek U, A Traditional Spice of Aceh NURLIANA NURLIANA; LISDAR IDWAN SUDIRMAN
HAYATI Journal of Biosciences Vol. 16 No. 1 (2009): March 2009
Publisher : Bogor Agricultural University, Indonesia

Pliek u obtained by traditionally fermentation of coconut meat is a potential source of antimicrobial compounds. This research was aimed to detect of their active compounds by bioautographic method and to analyze their chemical composition by GC-MS. For this purposes, pliek u was extracted with ethanol 96% to get crude ethanol extract of pliek u (EEP) and to get ethanol extract of residual pliek u (EERP) which was previously extracted by hexane. Crude EEP was separated into four bioautographic spots with different Rfs (0.93, 0.71, 0.19, and 0.10) which were all shown to be active against Staphyloccoccus aureus. Similar result was shown by EERP, but only resulted three bioautographic spots (Rfs 0.77, 0.63, and 0.4). Crude EEP consisted of 22 components representing 99.98% with fatty acids, ester, alcohol as major constituents and aliphatic hydrocarbon. EERP consisted of 9 components representing 99.80% with alcohol as major constituents and fatty acids, ester, 4-Dibenzofuramine and amine as minor constituents. The present of many active compounds in pliek u supports the use of pliek u as spice to improve the quality of food and encourages further studies to determine those active compounds. Key words: antimicrobe detection, chemical composition, pliek u

Detection of Antimicrobial Compounds Isolated from Several Tropical Lentinus by Bioautographic Method LISDAR I. SUDIRMAN
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

The antimicrobial compounds extracted either from culture filtrates or mycelia of several tropical Lentinus species could be detected their existences and locations by bioautographic method. For this purpose, the crude extracts were deposited as spots on silica gel plates and developed in a n-butanol-acetic acid-water mixture (3:1:1). The dry silica gel plates were then seeded with Bacillus subtilis and incubated at 35 oC for one night. On these plates, the extracts were separated into several bioautographic spots or growth inhibition zones. In parallel, the spots were detected by viewing with chemical revelations or under ultraviolet radiations at 254 nm or 366 nm. On silica gel thin-layer chromatograms, the crude extracts of Lentinus were separated into several bioautographic spots; for the filtrate extracts of L. squarrosulus 55A into three spots (Rfs 0.75, 0.50, 0.17), the mycelial extracts of L. sajor-caju LSC8 into two spots (Rfs 0.77, 0.54), the mycelial extract of L. torulosus LU3 into two spots (Rfs 0.77, 0.48), the filtrate extracts of L. cladopus LC6 into one spot (Rf 0.76) but the mycelial extracts of this mushroom separated into two spots (Rfs 0.79, 0.54), the filtrate and mycelial extracts of L. cladopus LC4 into three spots respectively (Rfs 0.75, 0.61, 0.45 for the filtrate extract and Rfs 0.83, 0.73, 0.60 for mycelial extract). By this method, the active compounds were detected directly and it is a usual method for further work on the purification of the target compounds.

Fungal Succession and Decomposition of Acacia mangium Leaf Litters in Health and Ganoderma Attacked Standings SAMINGAN SAMINGAN; LISDAR IDWAN SUDIRMAN
HAYATI Journal of Biosciences Vol. 16 No. 3 (2009): September 2009
Publisher : Bogor Agricultural University, Indonesia

Leaf litters of Acacia mangium play an important functional role in ecosystem, producing sources of nutrients and giving diversity of microorganisms. Understanding the variation in fungal populations in A. mangium forest is important due to the roles of fungi in regulating populations of other organisms and ecosystem processes. For these purposes, the tests were conducted under two years old of health standing (2S) and Ganoderma attacked standing (2G) using litterbag method. Litter weight loss and lignin, cellulose, C, N contents were measured each month during eight months of decomposition, as well as fungal community involved was observed. Litter weight loss and lignin, cellulose, C, N contents were measured each month during eight months of decomposition, as well as fungal community involved was observed. After eight months of decomposition, litter weight losses were low up to 34.61% (k = 0.7/year) in 2S and 30.64% (k = 0.51/ year) in 2G, as well as lignin weight losses were low up to 20.05% in 2S and 13.87% in 2G. However, cellulose weight losses were 16.34% in 2S and 14.71% in 2G. In both standings, the numbers of fungal species were 21 and 20 respectively, while the total of fungal populations tends to increase after one month of decomposition and tend to decrease in the last three months. In the first and second months of decomposition fungal species were dominated by genera of Penicillium and Aspergillus and the last three months by Trichoderma, Phialophora, and Pythium. Key words: fungal succession, decomposition, leaf litters, A. mangium

Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4 LISDAR IDWAN SUDIRMAN
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Lentinus cladopus LC4 produced at least eight antimicrobial compounds (ACs) which are active against plant and human pathogens. Three ACs in its crude mycelial were extracted with methanol and partial purification was carried out with silicic acid column chromatography and by thin layer chromatography (PTLC). The antimicrobial activity was tested by paper disc method and antibiographic method. The chromatography purification eluted with dichloromethane containing 5% methanol gave one active fraction (FII). This fraction which was active against X. campestris pv. glycines and showing two inhibition zones against Bacillus subtilis on bioautographic plates with the Rfs 0.8 and 0.7. FI and FIII fractions eluted with dichloromethane containing 0 and 10% methanol performed one inhibition zone with Rfs 0.8 and 0.7 respectively. However, their activities were lower than that of FII fraction. The PLTC purification gave one separate fraction with Rf value of 0.73 and it was active against X. campestris pv. glycines. The compound of Rf 0.73 fraction should be further studied using TLC and HPLC to obtain the pure substance for molecule characterization.

Co-Authors . RINDITA A.A. Ketut Agung Cahyawan W AUNU RAUF Azizah Nurmalinda Dian Syahfitri Didiek Hadjar Goenadi Evarini Anna Ratnaningsih Fahrizal Hazra Fitratul Aini Fitratul Aini, Fitratul HENNY SULISTANY Henny Sulistiany HENNY SULISTIANY, HENNY Kartosuwondo, Utomo Laksmita Prima Santi Maria M. Misnawati Melly Fitriani Mersi Kurniati Munara, Yayi Nabila Tsoerayya Gustia Pudjas NISA RACHMANIA MUBARIK NURLIANA NURLIANA Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Prayogo, Yusmani Rika Indri Astuti Ruly Anwar Samingan Samingan Santoso, Teguh SEMPURNA GINTING SEMPURNA GINTING Siti Azzira Rahma Siti Nikmatin Soemartono Sosromarsono Trizelia . Yonny Koesmaryono Yunilda Rosa YUNIMAR YUNIMAR Yusmani Prayogo

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