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All Journal Jurnal Teknologi Dan Industri Pangan Microbiology Indonesia Jurnal Kedokteran Meditek
Kris Herawan Timotius
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Public Health

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KANDUNGAN ANTOSIANIN DAN ANTOSIANIDIN DARI JANTUNG PISANG KLUTUK (Musa brachycarpa Back ) DAN PISANG AMBON (Musa acuminata Colla) [Anthocyanin Content and Identification of Anthocyanidin of Banana Bract Klutuk variety (Musa brachycarpa Back) and Ambo Lydia Ninan Lestario; Dhanu Lukito; Kris Herawan Timotius
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 2 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.228 KB)

Abstract

The aims of this research were to determine total anthocyanin content and to explore the type of anthocyanidin of banana bract klutuk variety and ambon variety. The total anthocyanin content was determined with pH differential method, the data were then statistically-analyzed with t-test with 5 replications, whereas the exploration of anthocyanidin was based on Rf value and maximum absorbance of the spots on TLC, and also time retention of the peak on HPLC equiped with anthocyanidin standards. The results showed that the total anthocyanin content of banana bract of klutuk variety 909,44 ± 280,53 mg/ 100 g; which was significantly different from  ambon variety 1515,40 ± 193,74 mg/ 100 g. The type anthocyanidin of banana bract of klutuk variety include delphinidin and cyanidin, whereas ambon variety were delphinidin, cyanidin, and pelargonidin.      
Optimum Concentration of Glucose and Orange II for Growth and Decolorization of Orange II by Enterococcus faecalis ID6017 under Static Culture V. IRENE MEITINIARTI; ENDANG S. SOETARTO; EKO SUGIHARTO; KRIS HERAWAN TIMOTIUS
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.255 KB) | DOI: 10.5454/mi.2.2.5

Abstract

Growth and decolorization performance of bacterial grown on azodyes-containing-medium is influenced by various concentrations of carbon sources and azodyes. The optimum level of glucose and Orange II concentration for growth and Orange II decolorization by Enterococcus faecalis ID6017 are reported in this paper. The experiments were carried out in liquid static culture as batch experiments. Glucose and Orange II concentrations used in these experiments were 0.45, 0.90, 1.80 g l-1, and 40, 80, 120 mg l-1, respectively. The specific growth rate and decolorization rate of Orange II by E. faecalis were highest on the medium which contained at least 0.90 g l-1 glucose. It is necessary to note that glucose above 0.90 g l-1 gave no significant difference. On the medium containing 0.90 g l-1 glucose and 80 mg l-1 Orange II, E. faecalis grew with the highest specific growth rate (0.28 h-1) and Orange II decolorization rate (0.47 h-1). The maximum specific growth rate of biomass (μmax) and the halfsaturation coefficient (KS) under optimal conditions were 0.25 h-1 and 1.5 g.l-1, respectively. The kinetics of decolorization indicated that the process followed first order kinetics with respect to the initial concentration of Orange II. The inhibition constant (KI) was found to be 750 mg l-1 Orange II, indicating that Orange II concentration at e” 750 mg l-1 would inhibit bacterial growth to decolorize Orange II..
Products of Orange II Biodegradation by Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016 VINCENTIA IRENE MEITINIARTI; ENDANG SUTARININGSIH SOETARTO; KRIS HERAWAN TIMOTIUS; EKO SUGIHARTO
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.229 KB) | DOI: 10.5454/mi.1.2.1

Abstract

Chryseobacterium indologenes and Enterococcus faecalis were isolated from activated sludge of textile wastewater treatment plant. These bacteria had the ability to decolorize several azo-dyes. Degradation of azo dyes was initiated by decolorization (reduction of azo bond) which occurred in anaerobic condition. In this study, we focussed on biodegradation of Orange II by pure culture of C. indologenes ID6016 and E. faecalis ID6017, and to determine the metabolite products of Orange II degradation. The degradation of Orange II by both bacteria was carried out in batch experiments using liquid medium containing 80 mg/l Orange II, under sequential static agitated incubation. During the bacterial growth under static incubation (6 h), 66.1 mg/l Orange II were decolorized by 35.54 mg/l biomass of E. faecalis ID6017, but no decolorization found with C. indologenes ID6016. Based on HPLC results, the decolorized Orange II products were identified as sulfanilic acid and amino-naphthol. These metabolites were probably used or degraded by C. indologenes ID6016 under agitated incubation.
Isolasi Methicillin Resistant Staphylococcus aureus pada Mikrofon yang Digunakan saat Aktivitas Perkuliahan di Fakultas Kedokteran dan Ilmu Kesehatan Ukrida Donna Mesina Pasaribu; Amelia Graciella Tjiptabudy; Kris Herawan Timotius
Jurnal Kedokteran Meditek Vol 26 No 3 (2020): SEPTEMBER - DESEMBER
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v26i3.1892

Abstract

Microphone is an audio tool that is generally used during lecture. Microphone is not only used by one individual, but also by many people, a potential source of bacterial spread. The purpose of this study was to determine the presence or absence of Methicillin Resistant Staphylococcus aureus in the microphones used at the Faculty of Medicine and Health Sciences, Krida Wacana Christian University (FKIK UKRIDA). Research was conducted by using a descriptive study method with a total sampling technique. Samples taken in 2019 were all microphones used in the lecture process at the FKIK UKRIDA. A total of 26 microphones were examined by means of performing a swab on each microphone and planted on mannitol salt agar media, then transferred to nutrient agar. It was then followed by a catalase testing, gram staining, mannitol sugar testing, and sensitivity test of Staphylococcus aureus bacteria against oxacillin antibiotics. Results showed that 11 microphones contained Staphylococcus aureus bacteria and, on the sensitivity test results, all bacteria grown were sensitive to oxacillin antibiotics. The study concludes that no Methicillin Resistant Staphylococcus aureus found on the microphones used during lecture activities in FKIK UKRIDA.
Co-Authors Amelia Graciella Tjiptabudy Dhanu Lukito Donna Mesina Pasaribu ENDANG S. SOETARTO ENDANG SUTARININGSIH SOETARTO Lydia Ninan Lestario Saiful Akhyar Lubis Vincentia Irene Meitiniarti Yusak Maryunianta