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- Suliantari
Southeast Asian Food and Agricultural Science and Tecnology Center, Institut Pertanian Bogor, Bogor; dan Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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Cronobacter sakazakii MEMASUKI KONDISI VIABLE BUT NONCULTURABLE SELAMA PEMBENTUKAN BIOFILM Yesica M. R. Sinaga; Ratih Dewanti-Hariyadi; - Suliantari
Jurnal Teknologi dan Industri Pangan Vol. 27 No. 2 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.059 KB) | DOI: 10.6066/jtip.2016.27.2.140

Abstract

Studies show that nonsporeformer food-borne pathogens may enter a viable but nonculturable (VBNC) state under stress conditions. This research aimed to study the ability of Cronobacter sakazakii to enter a VBNC state during biofilm formation on stainless steel (SS) surfaces and its resuscitability. C. sakazakii YRt2a pGFPuv mutant and wildtype (WT) originally isolated from powder infant formula (PIF) were used in this study. Biofilms were developed on SS surfaces in 1/10 Trypticase Soy Broth (TSB). Culturability of the biofilms was monitored by swabbing and plating the WT or mutant sessile cells onto Trypticase Soy Agar (TSA) or TSA containing 100 μg/mL ampicillin (TSAA), respectively. Meanwhile, their viability was measured using direct microscopic (DMC) count based on green fluorescence for mutant isolates and direct viable count (DVC) for the WT using a fluorescence microscope. Biofilm of C. sakazakii pGFPuv mutant on SS entered VBNC state after 25 days of incubation, while the WT C. sakazakii biofilms was still culturable until day 63. Sodium pyruvate in solid and liquid medium was not able to resuscitate the biofilm cells of C. sakazakii pGFPuv in VBNC state. C. sakazakii pGFPuv mutants enter VBNC state faster than the WT isolates. Depleted nutrient is thought to drive biofilm of C. sakazakii pGFPuv to enter VBNC.
SINTASAN Cronobacter sakazakii pGFPuv SELAMA PENYIMPANAN JAGUNG PIPILAN BERKADAR AIR AWAL BERBEDA DI BERBAGAI RH Karina Nola Sinamo; - Suliantari; Ratih Dewanti-Hariyadi
Jurnal Teknologi dan Industri Pangan Vol. 27 No. 2 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.529 KB) | DOI: 10.6066/jtip.2016.27.2.148

Abstract

Cronobacter sakazakii is an opportunistic foodborne pathogen reported to cause necrotizing enterocolitis, bacteremia, and meningitis in certain groups of infant. C. sakazakii has been reported to survive at low aw or dryness. Presence of wild-type C. sakazakii in dry product is difficult to be distinguished from naturally occuring C. sakazakii. A pGFPuv mutant of C. sakazakii has been reported to have similar growth pattern, thus has the potential to be used in further investigation. The study aimed to evaluate the effect of initial moisture content and relative humidity (RH) on the survival rate of C. sakazakii pGFPuv in corn kernels during storage at room temperature. The study consists of drying corn kernels to achieve moisture contents of 12 and 16% (w.b), inoculation of C. sakazakii pGFPuv, and storage at RH 50, 70 and 90% for 12 weeks. Every two week, corn kernels were sampled and the moisture content was measured using oven method, water activity was measured with aw meter, and total C. sakazakii pGFPuv was enumerated by spread plate method. Meanwhile, total bacteria, mold and yeast were enumerated by pour plate method. Corn kernels achieved equilibrium moisture content and aw after two weeks of storage. The number of C. sakazakii decreased rapidly during storage at RH 70 and 90%, however they could survived at RH 50% for 12 weeks, especially when the initial moisture content was 16%. The total bacteria decreased by 3.5-3.9 Log CFU/g during storage at three RHs, but mold and yeast increased rapidly at RH 90%.
Co-Authors Karina Nola Sinamo Ratih Dewanti -Hariyadi Yesica M. R. Sinaga