Darmadi Goenarso
Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung

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Konsumsi Oksigen, Kadar Hb Darah, Dan Pertumbuhan Ikan Mas, Cyprinus carpio, Diberi Pakan Campuran Ampas Kelapa Darmadi Goenarso; Suripto Suripto; K I Susanthi
Jurnal Matematika & Sains Vol 8, No 2 (2003)
Publisher : Institut Teknologi Bandung

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A laboratory experiment on carp (Cyprinus carpio) given a shredded coconut mixed diet has been conducted. Carp aged 4 – 5 months (n = 50; weight = 24,46-25,78 g), were kept individually in each aquarium (50x30x30 cm). The waste shredded coconut was mixed with commercial fish feed. Five concentrations (w/w) of mixed diet were given as treatment, i.e.: 30% (P30), 40% (P40), 50% (P50), and 60% (P60). Controlled fish was only given commercial feed. The experiment was done within 10 weeks with five replicates. Fish were fed twice daily as much as 10% of body weight. Measurements on oxygen consumption rate and body weight were done weekly. The fish were kept from food for 24 hours prior to measurements. The hemoglobin concentration was measured at the beginning and at the end of the experiment only. ANOVA was used for statistical tests. Results showed that the oxygen consumption rate of the treated fish was not different from the controlled fish. Relative growth rate decreased significantly. The average growth rate of fish treated with P30, P40, P50, P60, were respectively 0.042%, 0.041%, 0.031% and 0.018%. Growth rate of controlled fish was 0.104%. There was n o difference on the hemoglobin concentration measured at the end of the experiment (7.89 mg/dl) compared to the value at the start (7.91 mg/dl) of the experiment. It can be concluded that waste shredded coconut mixed diet might be used as fish feed. However, some improvements should be done on the composition of nutrition in the diet to increase the fish growth rate.
Efek Gosipol terhadap Kontraksi Usus Halus Mencit (Mus musculus) Swiss Webster Jantan secara in vitro Darmadi Goenarso; Suripto Suripto; Zulfiani Zulfiani
Jurnal Matematika & Sains Vol 9, No 1 (2004)
Publisher : Institut Teknologi Bandung

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The in vitro effect of gossypol on the small intestinal contractility of Swiss Webster mice have been examined. Thirty six sexually mature male mice with body weights of 30,5-36,5 g. were used and the tested concentrations of gossypol were 5, 7.5, and 10 ppm. Segments of the small intestine such as: duodenum, jejunum and ileum were isolated and immersed in Tyrode solution at 37 oC. The contractions of these organs were measured and recorded using a myograph and physiograph. As control, the contractions of these organs were recorded in Tyrode solution for 360 seconds. The solution was then replaced by gossypol-Tyrode solution and the contractions were recorded again for the next 360 seconds. The results showed that gossypol increased shortly and followed by gradual decreases of tension in all small intestinal segments. Gossypol also lowered the frequency of the intestinal contractions when compared with control. The strongest action of gossypol was found when the intestines were treated in 10 ppm, compared to the action of those treated with 7.5 ppm and 5 ppm. It is assumed that gossypol impaired peristaltic movements of the intestines and thus decreased digestibility of mice.