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Kualitas Daging Ikan Kurisi (Nemipterus japonicus) Hasil Tangkapan Nelayan di Pelabuhan Perikanan Branta, Pamekasan: Quality of Kurisi Meat (Nemipterus japonicus), Results of Fisherman Catching in Branta Fishing Port, Pamekasan Abdus Salam Junaedi; Fortunata Riana; Harfatia Chandra Puspita Sari; Witria Witria; Muhammad Zainuri
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 2 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i2.31169

Abstract

Quality control over the catch of fishermen in the Branta Fishery Port, Pamekasan is still not well-implemented. This study was aimed to evaluate the quality of kurisi fish meat based on the value of the Total Plate Count (TPC), diversity and total heterotrophic bacteria and pathogens on TSA, EMB, SSA, and TCBS media. The heterotrophic bacterial TPC (TSA) value was 8.59 Log CFU/g with 7 species diversity and the highest total of species abundance was between 2-336 colonies (BH3). The pathogenic bacterial TPC (EMB) value was 3.72 Log CFU/g with 6 species diversity and the highest total of species abundance was between 784-1009 colonies (BPE4). The BPE1 bacterial isolate which was metallic green was suspected to be Escherichia coli. The pathogenic bacterial TPC (SSA) value was 4.12 Log CFU/g with 5 species diversity and the highest total of species abundance was between 35-450 colonies (BPS1), and the BPS1 bacterial isolate which was black was suspected to be Salmonella sp. The pathogenic bacterial TPC (TCBS) value was 5.41 Log CFU/g with 2 species diversity and the highest total of species abundance was between 0-44 colonies (BPT1). The green and yellow BPT1 and BPT2 bacterial isolate were suspected to be Vibrio parahaemolyticus and Vibrio vulnificus.
UJI BAKU MUTU PROBIOTIK IKAN BERBAHAN DASAR AIR LIMBAH CUCIAN BERAS, KULIT BAWANG PUTIH (Allium sativum), DAN FERMENTASI EKSTRAK DAUN KELOR (Moringa oleifera) M. Atho'illah; Mara Dwi Fadila; Abdus Salam Junaedi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 17, No 4 (2021): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.17.4.240-246

Abstract

Probiotik merupakan mikroba hidup dengan sifat menguntungkan. Jumlah air limbah cucian beras sangat melimpah dan memiliki kandungan nutrisi yang cukup banyak, terutama karbohidrat sebesar 85-90%. Air limbah kulit bawang putih berfungsi untuk menghambat pertumbuhan bakteri seperti Escherichia coli, Staphylococcus aureus, dan Salmonella enteritidis. Daun kelor (Moringa oleifera) merupakan tumbuhan yang mempunyai kandungan protein sebesar 27-29%. Tujuan penelitian ini adalah mengevaluasi kombinasi terbaik probiotik yang dapat digunakan dalam budidaya perikanan guna meningkatkan pertumbuhan dan kualitas ikan. Penelitian ini menggunakan rancangan acak faktorial (RAF) 4x4 dengan 2 faktor. Faktor pertama berupa kultur fermentasi ekstrak daun kelor yang terdiri atas 4 perlakuan, yaitu 10%; 20%; 30%; dan 40% dengan kombinasi antara air limbah cucian beras dan air limbah kulit bawang putih sebesar 1:1. Faktor kedua berupa lama waktu fermentasi probiotik yang terdiri atas 4 perlakuan, yaitu 1; 2; 3; dan 4 minggu. Kontrol negatif berupa air limbah cucian beras dan kulit bawang putih dengan perbandingan 1:1 dan kontrol positif dengan penambahan probiotik EM4 berkonsentrasi 10%. Setiap perlakuan diuji menggunakan 3 pengulangan. Metode penelitian ini menggunakan metode observatif. Hasil penelitian menunjukkan bahwa probiotik ikan yang diamati menghasilkan pelet pada permukaan dasar wadah fermentasi, gelembung gas, perubahan warna menjadi cokelat kuning, perubahan bentuk wadah fermentasi, bau khas seperti bau tape, dan tidak adanya kontaminasi jamur. Probiotics are a living microbe with a beneficial nature. Rice dishwater is plentiful and has a good amount of nutrition, especially a carbohydrate of 85-90%. Garlic skin wastewater helps prevent the growth of bacteria such as Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. Kelor (Moringa oleifera) is a plant that has a protein content of 27-29%. The aim of this study is to evaluate the best possible combination of probiotics that can be used in fisheries cultivation to promote the growth and quality of fish. The study uses randomly vectorial design 4x4 with 2 factors. The first factor is the four treatments fermentation culture extract of kelor leaves, which is 10%; 20%; 30%; and 40% by the combination of rice dishwater and garlic wastewater by 1:1. The second factor is long-term probiotic fermentation, consisting of 4 treatments, 1; 2; 3; and 4 weeks. The negative control of rice and garlic skin dishwater by comparison 1:1 and positive control with an additional probiotic EM4 concentrates 10%. Each treatment is tested using three repetitions. This research method using observative. Research indicates that probiotic fish observed produce pellets on the ground surfaces of fermentation containers, gas bubbles, discoloration to yellow chocolate, fermentation containers change in shape, typically smells like tape, and an absence of fungal contamination. 
Nilai Total Plate Count (TPC) Dan Jumlah Jenis Bakteri Air Limbah Cucian Garam (Bittern) Dari Tambak Garam Desa Banyuajuh Kecamatan Kamal Kabupaten Bangkalan Nur Hamida Laili; Indah Wahyuni Abida; Abdus Salam Junaedi
Juvenil Vol 3, No 1 (2022)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v3i1.15075

Abstract

ABSTRAKAir limbah cucian garam (Bittern) mengandung berbagai macam mineral dan mikroorganisme halofilik. Keberadaan mikroba indigenous yang ada pada air limbah cucian garam belum banyak digali potensinya baik dari jumlah maupun jenisnya. Penelitian ini bertujuan untuk mengetahui nilai Total Plate Count (TPC) dan jumlah isolat mikroba dari air limbah cucian garam (Bittern) yang berasal dari tambak garam Desa Banyuajuh, Kec. Kamal, Kab. Bangkalan. Penelitian ini dilakukan dengan metode purposive sampling, dimana sampel bittern di ambil dari tambak garam desa Banyuajuh, Kec. Kamal dengan melakukan pengukuran salinitasnya terlebih dahulu. Metode penghitungan nilai total bakteri menggunakan metode Total Plate Count (TPC) dan untuk identifikasi bakteri dilakukan isolasi bakteri heterotroph pada media TSA. Hasil penghitungan TPC didapatkan pada seri pengenceran 10-5 yaitu 416 CFU/ mL, sementara pada seri pengenceran 10-6 didapatkan sebesar 157 CFU/ mL, dan seri pengenceran 10-7 didapatkan sebesar 114 CFU/ mL.Sedangkan hasil isolasi didapatkan 10 isolat jenis bakteri Bittern.Kata Kunci: Bakteri Halofilik, Limbah Cucian Garam (Bittern), Total Plate Count (TPC)ABSTRACTSalt washing wastewater (Bittern) contains a wide variety of minerals and halophilic microorganisms. The existence of indigenous microbes in salt washing wastewater has not been explored for its potential, both in terms of number and type. This study aims to determine the value of Total Plate Count (TPC) and the number of microbial isolates from salt washing wastewater (Bittern) originating from the salt ponds of Banyuajuh Village, Kec. Kamal, Kab. Bangkalan. This research was conducted by purposive sampling method, where bittern samples were taken from the salt ponds of Banyuajuh village, Kec. Kamal by measuring the salinity first. The method of calculating the total value of bacteria using the Total Plate Count (TPC) method and for bacterial identification, heterotroph bacteria were isolated on TSA media. The results of the TPC calculation were obtained in the 10-5 dilution series, namely 416 CFU/mL, while the 10-6 dilution series obtained 157 CFU/mL, and the 10-7 dilution series obtained 114 CFU/mL. While the isolation results obtained 10 isolates. Bittern bacteria. Keywords: Halophilic Bacteria, Salt Laundry Waste (Bittern), Total Plate Count (TPC)