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All Journal Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana
Yesy Febnica Dewi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Asosiasi Lokus Mikrosatelit BMS1282 dengan Kejadian Kawin Berulang pada Sapi Bali Yesy Febnica Dewi; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Bali cattle has an important role to fulfill the meat demands in Indonesia. The improvement of molecular technology will facilitated bali cattle genomic selection. The aim of this research was to verify association between BMS1282 microsatellite locus and repeat breeding in bali cattle. Totalling 52 bali cattle blood samples was taken from UD Sari Laba, Bangli, Bali as DNA resources. DNA was extracted using blood mini Kit qiagen and microsatellite locus was amplified by Polymerase Chain Reaction (PCR). Alleles were separated with 6% PAGE and visualized using silver stain. There were 5 alleles in BMS1282 locus which the alleles size varied from 148 to 156 bp. Allele 152 was the higest frequency (54.81%) and allele 156 was the lowest frequency (1.92%). The value of heterozygosity (Ho), He, and PIC was 0.346, 0.626, and 0.569. consecutively. Statistical analysis revealed that BMS1282 locus has no assosiation with the repeat breeding (P>0.05). This result indicates that BMS 1282 locus has no important role in regulating the repeat breeding.
Efektifitas Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Penurunan Kadar Glukosa Darah Tikus Putih Jantan (Rattus novergicus) Yang Di Induksi Aloksan Yesy Febnica Dewi; Made Suma Anthara; Anak Agung Gde Oka Dharmayudha
Buletin Veteriner Udayana Vol. 6 No.1 Pebruari 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun sirih merah (Piper crocatum) terhadap penurunan kadar glukosa darah tikus putih jantan (Rattus novergicus) yang diinduksi aloksan. Penelitian ini menggunakan 20 ekor tikus putih jantan  (Rattus novergicus) yang diadaptasi selama tiga minggu dan dibagi secara acak menjadi lima kelompok dan masing-masing kelompok berjumlah empat ekor. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL). Ada lima perlakuan yang diberikan sebagai berikut: perlakuan I sebagai kontrol diabetes tanpa diberikan perlakuan, perlakuan II sebagai kontrol negatif (aloksan 120 mg/kg bb), perlakuan III aloksan + suspensi ekstrak daun sirih merah 2% (dosis 50 mg/kg bb), perlakuan IV aloksan + suspensi ekstrak daun sirih merah 2% (dosis 100 mg/kg bb), perlakuan V sebagai kontrol positif (Aloksan + Glibenklamid 1 ml/kg bb). Hasil penelitian menunjukkan bahwa ekstrak daun sirih merah (Piper crocatum) 2% pada dosis 50 mg/kg bb, maupun dosis 100 mg/kg bb dapat menurunkan kadar glukosa darah tikus putih jantan (Rattus novergicus) dan ekstrak daun sirih merah (Piper crocatum) 2% memiliki efek yang sebanding dengan glibenklamid sebagai penurun glukosa darah.
Co-Authors Anak Agung Gde Oka Dharmayudha I Ketut Puja i Nengah Wandia Made Suma Anthara