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All Journal ANNALES BOGORIENSES YARSI Medical Journal Sainstek : Jurnal Sains dan Teknologi Makara Journal of Science
ITA DJUWITA
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Immunology & microbiology Medicine & Pharmacology Other

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Journal : Makara Journal of Science

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AKTIVITAS ANTIFUNGAL MINYAK ATSIRI JINTEN PUTIH TERHADAP Candida parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, DAN C. etchellsii MP18 Ridawati, Ridawati; Jenie, Betty Sri Laksmi; Djuwita, Ita; Sjamsuridzal, Wellyzar
Makara Journal of Science Vol. 15, No. 1
Publisher : UI Scholars Hub

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Abstract

Antifungal Activity of Cumin Oil Against Candida parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, and C. etchellsii MP18. Many kinds of spices are used in Indonesia, one of them is white cumin seed. This spice is used not only for cooking, but also for traditional medicine. This study reported of antifungal activity from white cumin’s essential oil. Extraction and identification of Cumin oil were carried out. We obtained 2.5-3.0% of white essential oil which was colorless or light yellow color. GCMS analysis revealed that there were 12 peaks. Based on peak’s intensity the oil were dominated by 4 compound i.e. cuminaldehide (35.44%), ρ-cymene (34.77%), β-pynene (15.08 %) and γ-terpinene (8.15%). Growth inhibition zone determination has been carried out by diffusion disc and direct method against yeast i.e. C. parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, and C. etchellsii MP18. The results showed that all of the yeasts were sensitive to cumin oil. The inhibition zone radius were 13.4-16.5 mm. The cumin oil showed the inhibition of yeast growth with MIC values of 0.028%-0.042% and MFC values 0.09%- 0.14%, while nystatin had MIC values 0.40%-0.50% and MFC values 3.0%-4.0%. The activity of cumin oil was very strong as antifungal.
Co-Authors Bambang Purwantara Betty Sri Laksmi Jenie Kaiin, Ekayanti Mulyawati Kusdiantoro Mohamad M Agus Setiadi Ridawati, Ridawati Roza Helmita TUTY LASWARDI YUSUF Wahono Esthi Prasetyaningtyas WELLYZAR SJAMSURIDZAL