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All Journal Dimas: Jurnal Pemikiran Agama untuk Pemberdayaan Sainstek : Jurnal Sains dan Teknologi
Roza Helmita
Mahasiswa Pascasarjana Institut Pertanian Bogor
Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Social Sciences Other

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POLA DISTRIBUSI MITOKONDRIA SEL-SEL TROFOBLAS BLASTOSIS MENCIT (Mus Muculus Albinus) DAN PENGARUHNYA TERHADAP KEGAGALAN HATCHING DAN IMPLANTASI Helmita, Roza; Djuwita, Ita; Purwantara, Bambang
Sainstek : Jurnal Sains dan Teknologi Vol 7, No 1 (2015)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.687 KB) | DOI: 10.31958/js.v7i1.121

Abstract

Implantation is the most critical stage in the establishment of pregnancy. In mammals, it has been estimated that between 25% and 60% of conceptuses are lost before or at the time of implantation. The objectives of  this study were to investigate  the ability of blastocyst hatching and attachment of trophoblast cells in in vitro, the outgrowth and differentiation of trophoblast cells in in vitro culture of hatched and non hatched blastocyst, the activity of mitochondria NADH-CoQ reductase and the pattern of mitochondrial distribution. Blastocysts were collected from mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatching within 24 hours, 48 hours and non hatching. Embryos were cultured in DMEM medium in 5% CO2 incubator at 37°C for 10 days. The trophoblasts monolayer were processed for Giemsa staining and histochemistry analysis of NADH-tetrazolium reductase activity. The outgrowth of trophoblast cells were measured using eyespiece micrometer. The results showed the ability of blastocyst hatching in in vitro were different. The trophoblast outgrowth diameter of 24 h hatched blastocyst was significantly different with the 48 h hatched and non hatched blastocyst. The activity of NADH-CoQ reductase of 24 h and 48 h hatched blastocysts showed higher intensity than the non hatched blastocysts (P<0,05) and the distribution of mitochondrial in trophoblast cell cytoplasma of 24 h and  48 h hatched blastocyst were homogen around nucleus whereas thoes of non hatched blastocyst were cluster and heterogen. In conclusion, in in vitro study the failure of blastocysts hatching and implantation was due to the failure of outgrowth and differentiation of the trofoblast cells and the impairment of NADH-CoQ reductase activity in complex I mitochondria to produce energy and the pattern of mitochondrial distribution.Key words: mitochondrial distribution, NADH- tetrazolium reductase, trophoblast, implantation 
Knowledge Enhancement Among Members of the Women Farmers Group (WFG) through Islamic Science-Based Soil Ecology Education Delfita, Rina; Helmita, Roza; Haviz, Muhammad; Fajar, Najmiatul; Marneli, Diyyan; Putra, Aidhya Irhash; KF, Dwi Rini; Aini, Latifah Zahra
Dimas: Jurnal Pemikiran Agama untuk Pemberdayaan Vol 23, No 2 (2023)
Publisher : LP2M of Institute for Research and Community Services - UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/dms.2023.232.18554

Abstract

Farmers' knowledge about soil ecology, which is a key factor in increasing the productivity of agricultural products, needs to be evaluated considering that various counseling efforts to increase agricultural yields have not shown satisfactory results. Farmers still unequally manage farmland, providing excessive chemical fertilizers without regard to soil ecology. The purpose of this education is to evaluate the knowledge of the Women's Farmers Group (WFG) before and after conducting soil ecology education based on Islamic science. The subjects of this education were 15 members of WFG Bunga Padi, Jorong Kawai, Nagari Batu Bulek, Lintau Buo Utara, Tanah Datar, West Sumatra, Indonesia. The community-based research (CBR) approach is used in this educational activity. The stages of educational activities start from socialization, laying the basis of the research and the research design, providing pretests and materials, gathering information and analyzing data on biological factors, capillarity of groundwater, water holding capacity, soil aeration, soil fertility, and soil pH, and the evaluation (post-test). The results show an increase in knowledge and understanding among WFG Bunga Padi members after Islamic science-based soil ecology education. It is hoped that, over time, their mindset in managing farmland will be balanced, with less reliance on chemical fertilizers and more emphasis on soil ecology. As a result, Islamic science-based soil ecology education could have a role in improving farmland and laying the foundation for organic farming.
Co-Authors Aidhya Irhash Putra Aini, Latifah Zahra Bambang Purwantara ITA DJUWITA KF, Dwi Rini M. Haviz Marneli, Diyyan Rina Delfita