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Wahyu Dwijani Sulihingtyas
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KAPASITAS ANTIOKSIDAN DAN KADAR FENOLIK TOTAL DALAM SARI BUAH MENGKUDU TERFERMENTASI Anak Agung Sagung Desy Dwi Martayani Martayani; Ni Made Suaniti; Wahyu Dwijani Sulihingtyas
Jurnal Kimia (Journal of Chemistry) Vol. 11. No.2 Juli 2017
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.256 KB) | DOI: 10.24843/JCHEM.2017.v11.i02.p02

Abstract

Penelitian ini tentang pengujian kapasitas antioksidan dan kandungan fenolik total sari buah mengkudu terfermentasi dengan penambahan sari jahe dan gula merah. Tujuan penelitian ini adalah untuk menganalisis perubahan kapasitas antioksidan dan kandungan fenolik total dalam sari buah mengkudu sebelum dan setelah difermentasi selama beberapa hari. Kapasitas antioksidan dalam sari buah mengkudu semakin kecil setelah fermentasi yaitu dari 38,10 mgGAE/100 g (sebelum ditambahkan sari jahe, gula merah, tanpa difermentasi) menjadi 30,18; 28,68; dan 23,97 mgGAE/100 g berturut-turut setelah 2, 4, dan 6 hari fermentasi. Penurunan juga terjadi pada kadar fenolik total yaitu dari 54,46 menjadi 45,54; 39,39; dan 34,82 mgGAE/100 g.
EFEKTIVITAS SISTEM PENGOLAHAN INSTALASI PENGOLAHAN AIR LIMBAH SUWUNG DENPASAR TERHADAP KADAR BOD, COD, DAN AMONIA Wahyu Dwijani Sulihingtyas; I W. Budiarsa Suyasa; Ni M. Indra Wahyuni
Jurnal Kimia (Journal of Chemistry) Vol. 4, No. 2 Juli 2010
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

The effectivity of waste water treatment systems in Suwung Waste Water Treatmant Installation Denpasar,was investigated, by analyzing its effluent Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD)and ammonia contents. The aims of the research are to determine the effectivity of each stage of waste watertreatment and the optimum detention time of waste water in aerated lagoon and sedimentation pond, in order toreduce the BOD, COD and ammonia concentration.The results showed that the effectivity of treatment systems of waste water in Suwung Denpasar was low.The percentages of the reduction of BOD5, COD and ammonia in content stage I were 14.29, 15.66, and 33.74%respectively. More over, the reduction percentages in stage II were 20.83, 14.43, and 17.39% respectively. On theother hand, the reduction percentages from the initial to final stages were 32.14% for BOD5, 27.83% for COD and45.26% for ammonia. The optimum detention time of waste water in aerated lagoon and sedimentation pond forreducing parameters was 5 day and 40 hour respectivel
ALTERNATIF PENGGUNAAN SILIKA BENTONIT SEBAGAI PENGGANTI FENOL-KLOROFORM-ISOAMIL ALKOHOL DALAM EKSTRAKSI DNA SECARA LANGSUNG DARI TANAH HUTAN MANGROVE Khisan Qamariya; Wahyu Dwijani Sulihingtyas; I Nengah Wirajana
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.151 KB) | DOI: 10.24843/JCHEM.2016.v10.i01.p06

Abstract

Research on comparing the metagenomic DNA extraction from mangrove forest soil with phenol-chloroform-isoamyl alcohol and with silica bentonite was conducted. The purpose of this study was to find the differences in quality and quantity of metagenomic DNA extracted. The silica bentonite was compared to meet the Green Chemistry concept. The metagenomic DNA from these extractions were analyzed by agarose gel electrophoresis and UV-Vis spectrophotometry. The results of electrophoresis showed that metagenomic DNA extracted with silica bentonite were relatively higher in concentration than with phenol-chloroform-isoamyl alcohol; but integrity of their DNA were the same. The results of UV-Vis spectrophotometry analysis showed that A260/230 ratio of the phenol-chloroform-isoamyl alcohol extract had higher relative purity level to humic acid. The A260/280 ratio of the final elution of the silica bentonite extraction showed the highest relative purity level to protein.
EKSTRAK DAUN SIRSAK (Annona muricata L.) SEBAGAI ANTIOKSIDAN PADA PENURUNAN KADAR ASAM URAT TIKUS WISTAR Ni Putu Rahayu Artini; Sri Wahjuni; Wahyu Dwijani Sulihingtyas
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 2 Juli 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation and identification of anti-oxidant  active isolate from soursop leave (Annona muricata L.) was carried out in this study. Methanol was used to macerate an 158 gram sample. This crude extract showed 77,22% of inhibition ability to reduce oxidation of DPPH (difenil-picrylhydrazine) at the fifth minute and 85,42% at the sixtieth minute inhibition time. Petroleum benzene, chloroform, n-buthanol, and water were applied to separate it. N-buthanol extract has the highest anti-oxidant reduction percentage which was 91,10% of inhibition ability to reduce oxidation at the fifth minute and 97,90% at the sixtieth minute inhibition time. From this research, it was obtained that the best dose of n-buthanol fraction applied to decrease uric acid was 200 mg/Kg BW with a percentage decrease of 86,29% (p=0,001).n-buthanol  fraction was identified by gas chromatography-mass spectrometer. The active compounds observed were: benzofuran,2,3-dihidro; tetradecana;  3-ethoxy-1,4,4a,5,6,7,8,8a-octahydroisoquinoline;  2-cyclohexen-1-one, 4-hydroxy-3,5,6-trimethyl-4-(3-oxo-1-butenyl). In conclusion, soursop leave (Annona muricata L.) extract  was active for decreasing uric acid of rat tested. For future research, this method could be applied to human in decreasing uric acid concentration in blood.
SINTESIS SILIKA GEL TERIMOBILISASI DIFENILKARBAZON DARI ABU SEKAM PADI MELALUI TEKNIK SOL GEL Ni Nyoman Sri Nopianingsih; I Wayan Sudiarta; Wahyu Dwijani Sulihingtyas
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.006 KB) | DOI: 10.24843/JCHEM.2015.v09.i02.p13

Abstract

A research on synthesis diphenylcarbazone immobilized silica gel from rice husk ash by sol-gel technique has been conducted. The purpose of this research was to synthesize the diphenylcarbazone immobilized silica gel from rice husk ash by sol-gel technique.  This research included modification of silica gel of rice husk ash and silica gel characterization by IR spectra and X-RD. The results showed that the diphenylcarbazone immobilized silica gel has been successfully synthesized. It can be seen from the FTIR spectrum that silica-imobilized revealed peaks at wave numbers of ±1087.85cm-1 indicating the presence of Si-O-Si. The peak seen at wave numbers of  ±952.84cm-1 indicating the presence of Si-O, at ±3361.93cm-1due to the Si-OH bond which was the main functional group  of silica gel. Another peaks at ±3313.71cm-1, ±800.46cm-1, ±1602.85cm-1,±1700 cm-1, and ±1602.85cm-1 suggesting the presence of N-H, aromatic C-H (substituted benzene), aromatic C=C, N=N and C=O stretching. X-RD data showed that the structure of diphenilcarbazone immobilized silica gel was amorphous.
BIOSORPSI Cr(III) PADA BIOSORBEN SERAT SABUT KELAPA HIJAU TERAMOBILISASI EDTA I Wayan Sudiarta; Wahyu Dwijani Sulihingtyas
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 1 Januari 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Studies on biosorption of Cr(III) on coconut fiber immobilized with EDTA biosorbent (B-EDTA) have been conducted. These studies included the optimization of EDTA immobilization on biosorbent, optimization of Cr(III) biosorption on B-EDTA biosorbent that include pH dan contact time biosorption, and also studied the mechanisms of Cr(III) interactions on biosorbent B-EDTA by squential desorption.The result showed that the optimum EDTA immobilization on biosorbent E-EDTA occured at ratio of 1mmol EDTA : 4g biosorbet (0,25mmol/g ), with the surface acidity of biosorbent B-EDTA of 2,18 mmol/g. Optimum biosorption process of Cr(III) on biosorbent B-EDTA occured at pH = 3, contact time of 120 minutes, with the biosorption capacity of Cr(III) on biosorbent B-EDTA of 8,32 mg/g. The squential desorption studies show that the interaction of Cr(III) on biosorbent B-EDTA occured predominantly through physical adsorption namely Van der Waals bonding.
Co-Authors Anak Agung Sagung Desy Dwi Martayani Martayani I Nengah Wirajana I W BUDIARSA SUYASA I Wayan Sudiarta Khisan Qamariya Ni M. Indra Wahyuni Ni Made Suaniti Ni Nyoman Sri Nopianingsih Ni Putu Rahayu Martini Sri Wahjuni