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All Journal Quagga CHEMPUBLISH JOURNAL Klinikal Sains (Jurnal Analis Kesehatan) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Ilmiah Farmako Bahari Nusantara Science and Technology Proceedings Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal Teknologi Kesehatan
Fitri Fadhilah
Sekolah Tinggi Analis Bakti Asih
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THE EFFECT OF TEST TUBE STERILIZATION FROM SERUM LIPEMIC AGAINST LEVELS OF TRIGLYCERIDE GPO-PAP METHOD Fitri Fadhilah; Ana Bina Sari; Astika Aprilianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.878

Abstract

In terms of analytic factors, it is important to define acceptable levels of common interferences, such as lipemia or hemolysis. For triglyceride, the laboratory technician must define whether samples with excess lipemia will be included in the study; this depends, in part, on whether the interferences affect the methods. In most laboratories, glass or plastic that is in direct contact associated with bio hazardous material is usually disposable. If not, it must be decontaminated according to appropriate protocols. Immediately rinsing glass or plastic supplies after use, followed by washing with a powder or liquid detergent designed for cleaning laboratory supplies and several distilled water rinses, may be insufficient. To ensure that all remaining fat from lipemic serum that attached to the tube wall has been removed, then the sterilization process is carried out so that a sterile tube is obtained. The purpose of this study is to determine the effect of test tube sterilization from serum lipemic against levels of triglyceride GPO-PAP (Glycerol-3-phosphate oxidase-p-aminophenazone) examination. This research method was a laboratory experiment. We used 8 times repetition with tubes used first are given liquid fat and cleaned by sterilization, washed with surfactant and washed with water only. By using statistical tests ANOVA obtained of this study showed results p>0.05 which is mean the treatment that used did not show a significant difference in the treatment of ordinary water-washed tubes with sterilized tubes and surfactant washed tubes. The conclusion of this study is cleaning of the test tube with the sterilization method is recommended because to avoiding the fear of remaining pollutants that can affect the results, It can also minimize the life of bacteria and viruses from the sample to be examined. However, if the sterilization method is difficult to do because of limited equipment and so on, the use of surfactants and the correct method of cleaning the tube is enough to remove impurities such as fat.
COMPARISON OF GLUCOSE REDUCTION IN URINE USING BENEDICT METHOD HEATED BY METHYLATED FLAME WITH 100°C WATERBATH Fitri Fadhilah; Noviana Vanawati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1075

Abstract

The high prevalence of Diabetes Melitus (DM) is a global problem that must be solved by health workers around the world. This study aims to determine the differences in the results of urine reduction examination using benedict method heated by spirtus flame and waterbath 100°C. This research method is a laboratory experiment. The results of this study indicated that the urine reduction examination by heating methylated flame and waterbath 100oC shows the same results from negative (-) until positive (+4). Examination of urine reduction by heating the methylated flame and waterbath 100oC did not affect the results. However, there are a difference in the process of urine reduction examination by heating flame which was need a longer time up to 3-5 minutes. Additionally, the solution in the tube could be exploded. It was also time consuming which could only carry out one by one sample. Meanwhile, the heating of urine by using 100oC waterbath is relatively faster, which only took 2 minutes. The urine was not also exploded when it was boiled and the heating process could perform 6-8 samples at the same time (depending on the tube rack). In conclusion, the heating method of urine by using waterbath was better than spirtus (methanol) flame since it could carry out large sample in one time and it was safer for the laboratory personnel.
Effect of Varied Concentrations of Ethylhene Glycol as Pooled Serum Preservative on the Stability of Alkaline Phosphatase (ALP) Ezyme Fitri Fadhilah; Sandra A. Riyadi; Euis Yuliani
Nusantara Science and Technology Proceedings Federation of Islamic Medical Associations
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2204

Abstract

Quality controls aim to ensure the quality, accuracy, and thoroughness of an examination. Internal quality assurance is carried out by the laboratory itself using control materials, both commercial control materials and homemade control materials. This controlled material is then used to ensure the quality of an inspection. The stability of the control material is strongly influenced by the storage temperature where the recommended temperature is -20? which will be stable for up to 6 months and 2-8? which is stable for 6 days. In the manufacture of homemade control materials, a stable preservative, antifreeze, and antibacterial is needed but still maintains the integrity of the group serum. The preservative used is ethylene glycol. This study aims to determine the optimal concentration of ethylene glycol in stabilizing alkaline phosphatase levels in refrigerator temperature storage (4-8ºC) for 60 days. In this study, tests were conducted on pooled sera preserved with ethylene glycol in varying concentrations, namely 7.5%; 10%; 12.5%; 15%; and 17.5% and observed the stability of the Alkaline Phosphatase enzyme in pooled sera for 60 days with an interval of 10 days with a storage temperature of 4-8?. The study was conducted in July-August 2021 in the BLUD laboratory of RSUD dr. Ben Mboi, Manggarai. The results showed that the stability of alkaline phosphatase persisted until day 30, began to decrease on day 40, and continued to decrease until day 60 in each different concentration variation. different, namely 7.5%; 10%; 12.5%; 15%; and 17.5% had the same stability up to 30 days. The recommendation from the author is to observe at different temperatures so that the stability of Alkaline phosphatase can be known.
PENGARUH LAMANYA PENCAHAYAAN TERHADAP KADAR BILIRUBIN TOTAL METODE KOLORIMETRIC DIAZO fitri fadhilah
Klinikal Sains : Jurnal Analis Kesehatan Vol 7 No 1 (2019): JUNI
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.715 KB) | DOI: 10.36341/klinikal_sains.v7i1.597

Abstract

Pemeriksaan laboratorium klinik merupakan system yang dapat menentukan keputusan mengenai suatu diagnosis penyakit melalui hasil laboratorium. Pemeriksaan laboratorium klinik dengan hasil yang berkualitas sangat diperlukan, salah satu pemeriksaan laboratorium yang harus dijaga kualitasnya adalah tentang penanganan sampel. Penanganan sampel yang baik memberikan hasil penanganan spesimen yang akurat, pemeriksaan yang membutuhkan penanganan sampel yang baik seperti pada pengukuran bilirubin karena mudah berubah ke stabilannya sehingga perlu pemeriksaan segera. Jenis penelitian ini merupakan eksperimen yaitu melakukan pemeriksaan laboratorium untuk mengetahui stabilitas kadar bilirubin total serum yang terpapar cahaya dan tidak terpapar cahaya selama 3 jam. Berdasarkan uji Anova didapatkan hasil sampel yang terpapar cahaya di 30 menit pertama penurunan kadar bilirubin sekitar 4% sedangkan setelah 1 jam penurunan sebanyak 10% dan pada waktu 3 jam penurunan kadar bilirubin total pada sampel sebesar 25%. Kesimpulannya yaitu, terdapat pengaruh yang signifikan terhadap lamanya serum yang terpapar cahaya, cahaya saja dapat mempengaruhi kadar bilirubin total serum sebanyak 10 persen dalam kurun waktu 1 jam dan 25 persen dalam kurun waktu 3 jam, dan waktu maksimal yang dibutuhkan dalam mempertahankan kadar bilirubin hanya sekitar 30 menit.
Perbandingan Reduksi Glukosa Pada Urin Menggunakan Pemanasan Api Spirtus Dan Waterbath 100oC Dengan Metode Benedict Anita Kurniyawati; Fitri Fadhilah; Anggi Nopiani
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 15 No. 1 (2019): Juni
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.424 KB)

Abstract

The high prevalence of DM is a global problem that must be handled by health workers around the world. The number of DM patients in the world in all age groups was 382 million people in 2013 and is estimated to increase 55% to 592 million sufferers in 2035. In Indonesia, provinces with the highest prevalence of DM are D.I. Yogyakarta with 2.6%, both Jakarta 2.5%, and third North Sulawesi with 2.4%. The prevalence of DM in South Kalimantan was ranked 13th at 1.4%. This study aims to determine the differences in the results of urine reduction examination benedict method using spirtus flame with waterbath 100° C. This research method is a laboratory experiment. The results of this study indicate that the urine reduction examination by heating flame flame and waterbath 100oC shows the same results from negative (-) until positive (+4). Examination of urine reduction by heating the flame of spirits and waterbath 100oC does not affect the results. But there is a difference in the process with heating flame methylate a longer time is 3-5 minutes, when boiling the solution in the tube explodes and can only work one by one sample, while the heating of 100oC waterbath the time required is relatively faster, 2 minutes, not explode when boiling, and can work 6-8 samples (depending on the tube rack you have). If the number of patients is much better the urine reduction examination benedict method uses a 100oC waterbath heating.
Efektifitas Suhu Dan Lama Penyimpanan Pada Preparasi Sampel Darah Terhadap Volume Serum Pada Pemeriksaan Kadar Glukosa Puasa, Kolesterol Total Dan Trigliserida Fitri Fadhilah; Ani Riyani; Anggi Nopiani
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 15 No. 2 (2019): November
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.422 KB)

Abstract

This research is aimed to determine whether any differences between the volume of serum and the result of fasting glucose level test, total cholesterol, and triglycerides on the serum achieved by temperature variation and storage duration. This is an experimental research that has been done on October 2012 at Clinical Laboratory. From the research, there are average storage amounts of serum volume for 10 until 30 minutes on 80C raised to 93,4%, on 250C from the storage in 10 until 30 minutes raised to 138,8%, and on 370C from the storage 10 until 30 minutes raised to 30%. Based on statistical calculations derived Sig. (2-tailed) of <1.000, for the Sig. > 0.05 showed no significant correlation to storage time 10 minutes, 20 minutes and 30 minutes. ANOVA’s test result indicated that grade F achieved is less from grade F critical, that means there are no differences or meaningful effects from temperature and storage duration treatments to the result of fasting glucose level test, total cholesterol, and triglycerides . There is the increasing of serum volume after the temperature treatment is given and the storage duration on blood samples preparation. Moreover, there are no significant differences for the result of fasting glucose test, total cholesterol, and triglycerides.
Penyimpanan Bakteri Escherichia coli Dan Streptococcus pneumonie Pada Media Cryoprotective Dengan Metode Freeze Drying Yati supriatin supriatin; Fitri Fadhilah Fadhilah; Vanessa Ayu Sumirat
Quagga : Jurnal Pendidikan dan Biologi Vol 12, No 1 (2020)
Publisher : Program Studi Pendidikan Biologi, FKIP Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v12i1.2148

Abstract

Proses perbanyakan dan penyimpanan bakteri menjadi hal yang harus diperhatikan karena cara penyimpanan sangat terkait dengan perubahan sifat bakteri yang disimpan. Di Laboratorium, kultur bakteri murni dipindahkan dengan metode subkultur. Kekurangan subkultur  berulang adalah memakan waktu, beresiko kontaminasi dan mutasi genetik. Penelitian ini bertujuan untuk mengembangkan penyimpanan bakteri jangka panjang yang aplikatif di Laboratorium Mikrobiologi serta menentukan jenis Cryoprotectan Agent yang dapat mempertahankan viabilitas sel Escherichia coli dan Streptococcus pneumonie dengan metode Freeze Drying. Metode penelitian yang digunakan adalah eksperimen dengan menambahkan Skim Milk 10% dan gliserol 10% sebagai Cryoprotectan Agent pada pelet sel Escherichia coli dan Streptococcus pneumonie dengan metode Freeze Drying. Digunakan kontrol tanpa penambahan Cryoprotectan Agent dengan metode Freeze Drying dan kontrol tanpa penambahan Cryoprotectan Agent  yang disimpan dalam refrigator (non Freeze Drying) kemudian jumlah koloni bakteri dihitung setelah masa simpan 2,4,6 minggu.Selanjutnya data yang diperoleh diolah secara statistik menggunakan metode statistik non parametrik kruskal wallis . Hasil penelitian menunjukkan bahwa Skim Milk 10% mampu mempertahankan viabilitas Sel Escherichia coli dengan persentase penurunan viabilitas paling rendah yaitu 4,2%, namun  jenis Cryoprotectan agent metode Freeze Dry belum mampu mengawetkan dan mempertahankan viabilitas sel Streptococcus pneumonie.Kata Kunci: Cryoprotectan Agent; Cell viability; Escherichia coli;, Streptococcus pneumonie; Freeze   Drying
AKTIVITAS ANTIKANKER KURKUMINOID TERHADAP SEL MELANOMA B16-F10 Sandra Amalia Riyadi; Fajar Fauzi Abdullah; Fitri Fadhilah; Nurul Assidiqiah
Jurnal Ilmiah Farmako Bahari Vol 13, No 2 (2022): Jurnal Ilmiah Farmako Bahari
Publisher : Fakultas MIPA Universitas Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jfb.v13i2.1458

Abstract

Kurkuminoid berasal dari rimpang kunyit (Curcuma Longa L.) yang memiliki ketiga komponen utama penyusunnya diantaranya kurkumin, demetoksikurkumin dan bisdemetoksikurkumin. Secara struktur ketiganya hanya berbeda pada subtituen gugus metoksi, namun mampu memberikan efek biologis yang berbeda. Kurkumin dalam beberapa tahun kebelakang menjadi salah satu target pengembangan penelitian sebagai agen antikanker yang dilakukan terhadap beberapa sel kanker. Namun, kemampuan kurkumin sebagai antikanker menjadi sangat terbatas akibat rendahnya kelarutan kurkumin dalam air, yang mempengaruhi rendahnya penyerapan senyawa kurkumin oleh sel dalam tubuh. Maka dari itu menjadi sangat perlu dilakukannya penelitian lebih lanjut untuk melihat aktivitas antikanker dari senyawa kurkumin lainnya yaitu demetoksikurkumin dan bisdemetoksikurkumin. Rimpang kunyit yang telah dikeringkan kemudian dimaserasi dengan etanol selama 3x24 jam kemudian maserat yang diperoleh diuapkan dengan rotary evaporator sehingga didapatkan ekstrak pekat etanol kunyit. Selanjutnya dilakukan partisi dengan pelarut n-heksan, etil asetat, dan n-butanol. Fraksi etil asetat kemudian dipekatkan dan dilakukan berbagai jenis metode pemisahan sehingga didapatkan ketiga senyawa kurkuminoid. Selanjutnya, isolat yang sudah terkarakterisasi di lakukan uji sitotoksik terhadap sel B16-F10 dengan menggunakan Metode Reduksi Resazurin. Hasil penelitian menunjukkan adanya perbedaan aktivitas penghambatan yang signifikan diantara ketiga isolat. Bisdemetoksikurkumin memiliki aktivitas penghambatan tertinggi dengan nilai IC50 16,20 µg/mL. Setelah itu, demetoksikurkumin dengan nilai IC50 22,59 µg/mL dan yang terakhir adalah kurkumin dengan nilai IC50 152,71 µg/mL.
PENGARUH HEMOLISIS DALAM SERUM TERHADAP AKTIVITAS ENZIM ASPARTAT AMINOTRANSFERASE DENGAN METODE KINETIK-IFCC Hilda Dila Wanti; Fitri Fadhilah; Opik Taufiqurrohman
Journal of Indonesian Medical Laboratory and Science Vol 1 No 1: Oktober 2020
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v1i1.6

Abstract

The aspartate aminotransferase (AST) enzyme is a mitochondrial enzyme that functions to catalyze the alternating transfer of amino groups from aspartic acid to ?-oxaloacetic acid to form glutamic acid and oxaloacetic acid. AST is present in erythrocytes so that if hemolysis occurs, the erythrocytes rupture will cause an increase in the activity of the AST enzyme that comes out of erythrocytes. This study aims to determine the effect of hemolysis in serum on the examination of AST enzyme activity. This research method is experimental by comparing normal serum with serum hemolysis. The research sample involved 16 students who would be examined for AST enzyme activity. The results showed that the activity of AST enzymes in normal serum had an average of 24.63 IU / L whereas in serum hemolysis there was an average of 39.38 IU / L. The results of the data showed that there was an increase in AST enzyme activity in serum hemolysis compared to normal serum. The data were then analyzed using the T test, and the Sig. 0.001 where p <0.05 proved that AST enzyme activity in normal serum and serum hemolysis was stated to be significantly different.
Pewarnaan Preparat Apus Tipis Malaria (Plasmodium vivax Dan Plasmodium falcifarum) Menggunakan Ekstrak Murbei Sebagai Pengganti Eosin Pada Komposisi Giemsa Sandra Amalia Riyadi; Fitri Fadhilah
CHEMPUBLISH JOURNAL Vol. 6 No. 2 (2021): Chempublish Journal
Publisher : Department of Chemistry, Faculty of Science and Technology Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Malaria is caused by the plasmodium parasite and still a threat to Indonesia. The main examination is staining of thick and thin smear preparations using Giemsa. Because giemsa is expensive, its waste is difficult to decompose, toxic and rare. Therefore, there is a need for new inventions to utilize the potential of natural components like mulberry fruit, which contains anthocyanins for coloring agents, to lessen the impact and risk of giemsa. The purpose of this study was to determine the effect of macerated ethanol extract of mulberry with a ratio of 1:3 (w/v) as an alternative to eosin in Giemsa on staining of thin blood smears of malaria, as well as knowing the best concentration in staining. This type of experimental research by observing the clarity of shape, color of parasites P.vivax, P.falcifarum, erythrocyte cells also compared with 3% giemsa as a control. This study consisted of 32 samples, 8 repetitions and 4 treatments namely P1 (3% Giemsa control), Giemsa modified mulberry eosin P2 (20%), P3 (30%), P4 (40%). The quality of the preparations was assessed based on the clarity and contrast of the colors. Kruskal-Wallis and Mann-Whitney data analysis techniques were used. The results showed that mulberry extract could be an alternative dye to replace eosin in the composition of giemsa on malaria thin smear preparations. The most effective concentration was 30% which could give the Plasmodium nuclei the same quality of staining as the control, while for the erythrocytes the staining quality was not the same as the control.
Co-Authors Ana Bina Sari Anggi Nopiani Ani Riyani Anita Kurniyawati Astika Aprilianti Euis Yuliani Fajar Fauzi Abdullah Hilda Dila Wanti Noviana Vanawati Nurul Assidiqiah Opik Taufiqurrohman Sandra A. Riyadi Sandra Amalia Riyadi Vanessa Ayu Sumirat Yati supriatin supriatin