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All Journal Quagga CHEMPUBLISH JOURNAL Klinikal Sains (Jurnal Analis Kesehatan) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Ilmiah Farmako Bahari Nusantara Science and Technology Proceedings Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal Teknologi Kesehatan
Fitri Fadhilah
Sekolah Tinggi Analis Bakti Asih
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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

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THE EFFECT OF TEST TUBE STERILIZATION FROM SERUM LIPEMIC AGAINST LEVELS OF TRIGLYCERIDE GPO-PAP METHOD Fitri Fadhilah; Ana Bina Sari; Astika Aprilianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.878

Abstract

In terms of analytic factors, it is important to define acceptable levels of common interferences, such as lipemia or hemolysis. For triglyceride, the laboratory technician must define whether samples with excess lipemia will be included in the study; this depends, in part, on whether the interferences affect the methods. In most laboratories, glass or plastic that is in direct contact associated with bio hazardous material is usually disposable. If not, it must be decontaminated according to appropriate protocols. Immediately rinsing glass or plastic supplies after use, followed by washing with a powder or liquid detergent designed for cleaning laboratory supplies and several distilled water rinses, may be insufficient. To ensure that all remaining fat from lipemic serum that attached to the tube wall has been removed, then the sterilization process is carried out so that a sterile tube is obtained. The purpose of this study is to determine the effect of test tube sterilization from serum lipemic against levels of triglyceride GPO-PAP (Glycerol-3-phosphate oxidase-p-aminophenazone) examination. This research method was a laboratory experiment. We used 8 times repetition with tubes used first are given liquid fat and cleaned by sterilization, washed with surfactant and washed with water only. By using statistical tests ANOVA obtained of this study showed results p>0.05 which is mean the treatment that used did not show a significant difference in the treatment of ordinary water-washed tubes with sterilized tubes and surfactant washed tubes. The conclusion of this study is cleaning of the test tube with the sterilization method is recommended because to avoiding the fear of remaining pollutants that can affect the results, It can also minimize the life of bacteria and viruses from the sample to be examined. However, if the sterilization method is difficult to do because of limited equipment and so on, the use of surfactants and the correct method of cleaning the tube is enough to remove impurities such as fat.
COMPARISON OF GLUCOSE REDUCTION IN URINE USING BENEDICT METHOD HEATED BY METHYLATED FLAME WITH 100°C WATERBATH Fitri Fadhilah; Noviana Vanawati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1075

Abstract

The high prevalence of Diabetes Melitus (DM) is a global problem that must be solved by health workers around the world. This study aims to determine the differences in the results of urine reduction examination using benedict method heated by spirtus flame and waterbath 100°C. This research method is a laboratory experiment. The results of this study indicated that the urine reduction examination by heating methylated flame and waterbath 100oC shows the same results from negative (-) until positive (+4). Examination of urine reduction by heating the methylated flame and waterbath 100oC did not affect the results. However, there are a difference in the process of urine reduction examination by heating flame which was need a longer time up to 3-5 minutes. Additionally, the solution in the tube could be exploded. It was also time consuming which could only carry out one by one sample. Meanwhile, the heating of urine by using 100oC waterbath is relatively faster, which only took 2 minutes. The urine was not also exploded when it was boiled and the heating process could perform 6-8 samples at the same time (depending on the tube rack). In conclusion, the heating method of urine by using waterbath was better than spirtus (methanol) flame since it could carry out large sample in one time and it was safer for the laboratory personnel.
Co-Authors Ana Bina Sari Anggi Nopiani Ani Riyani Anita Kurniyawati Astika Aprilianti Euis Yuliani Fajar Fauzi Abdullah Hilda Dila Wanti Noviana Vanawati Nurul Assidiqiah Opik Taufiqurrohman Sandra A. Riyadi Sandra Amalia Riyadi Vanessa Ayu Sumirat Yati supriatin supriatin