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Journal : Jurnal Teknologi Kesehatan

Perbandingan Reduksi Glukosa Pada Urin Menggunakan Pemanasan Api Spirtus Dan Waterbath 100oC Dengan Metode Benedict Anita Kurniyawati; Fitri Fadhilah; Anggi Nopiani
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 15 No. 1 (2019): Juni
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

The high prevalence of DM is a global problem that must be handled by health workers around the world. The number of DM patients in the world in all age groups was 382 million people in 2013 and is estimated to increase 55% to 592 million sufferers in 2035. In Indonesia, provinces with the highest prevalence of DM are D.I. Yogyakarta with 2.6%, both Jakarta 2.5%, and third North Sulawesi with 2.4%. The prevalence of DM in South Kalimantan was ranked 13th at 1.4%. This study aims to determine the differences in the results of urine reduction examination benedict method using spirtus flame with waterbath 100° C. This research method is a laboratory experiment. The results of this study indicate that the urine reduction examination by heating flame flame and waterbath 100oC shows the same results from negative (-) until positive (+4). Examination of urine reduction by heating the flame of spirits and waterbath 100oC does not affect the results. But there is a difference in the process with heating flame methylate a longer time is 3-5 minutes, when boiling the solution in the tube explodes and can only work one by one sample, while the heating of 100oC waterbath the time required is relatively faster, 2 minutes, not explode when boiling, and can work 6-8 samples (depending on the tube rack you have). If the number of patients is much better the urine reduction examination benedict method uses a 100oC waterbath heating.
Efektifitas Suhu Dan Lama Penyimpanan Pada Preparasi Sampel Darah Terhadap Volume Serum Pada Pemeriksaan Kadar Glukosa Puasa, Kolesterol Total Dan Trigliserida Fitri Fadhilah; Ani Riyani; Anggi Nopiani
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 15 No. 2 (2019): November
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

This research is aimed to determine whether any differences between the volume of serum and the result of fasting glucose level test, total cholesterol, and triglycerides on the serum achieved by temperature variation and storage duration. This is an experimental research that has been done on October 2012 at Clinical Laboratory. From the research, there are average storage amounts of serum volume for 10 until 30 minutes on 80C raised to 93,4%, on 250C from the storage in 10 until 30 minutes raised to 138,8%, and on 370C from the storage 10 until 30 minutes raised to 30%. Based on statistical calculations derived Sig. (2-tailed) of <1.000, for the Sig. > 0.05 showed no significant correlation to storage time 10 minutes, 20 minutes and 30 minutes. ANOVA’s test result indicated that grade F achieved is less from grade F critical, that means there are no differences or meaningful effects from temperature and storage duration treatments to the result of fasting glucose level test, total cholesterol, and triglycerides . There is the increasing of serum volume after the temperature treatment is given and the storage duration on blood samples preparation. Moreover, there are no significant differences for the result of fasting glucose test, total cholesterol, and triglycerides.