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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Buletin Penelitian Tanaman Rempah dan Obat BERITA BIOLOGI Buletin Penelitian Tanaman Rempah dan Obat
Fajarudin Ahmad
Lembaga Ilmu Pengetahuan Indonesia (LIPI), Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Veterinary

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PENGARUH ORYZALIN TERHADAP TINGKAT PLOIDI TANAMAN GARUT (Maranta arundinacea L.) L. Agus Sukamto; Albertus H. Wawo; Fajarudin Ahmad
Buletin Penelitian Tanaman Rempah dan Obat Vol 21, No 2 (2010): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v21n2.2010.%p

Abstract

The Effect of Oryzalin on Ploidy Level of Arrowroot Plant (Maranta arundinacea L.)Arrowroot is propagated vegetative so its genetic variation is very narrow. The narrow genetic variation could be broadened through ploidy manipulation. Oryzalin could induce ploidy level of chromosomes and broaden plant genetic variation. Increase in chromosome number usually corresponds with increase stomata, corm, and starch contents. The aims of this research were to broaden germplasms of arrowroot plant by doubling its chromosomes and seeking for individual plants that potentially poly-ploidy to yield increase. This experiment had been done in experimental garden of Cibinong Science Center, February-December 2009. A five-node rhizome was soaked in Oryzalin solution of 0; 10; 20; 30; 40; 50; and 60 µM for 6 days and washed with water. The rhizome was then grown in a polybag containing soil and compost (1:1) in field with 30% shaded net. Oryzalin treatment at high concentration on arrowroot rhizome inhibited plant growth but it increased plant growth at low concentration (10 µM). Several arrowroot plants resulted from Oryzalin treatments were potential polyploid plants. Their stomata were bigger/longer, greener, more rounded, thicker, and more undulated leaves than the control. 
Keragaman Genetik Kultivar Pisang Diploid (AA) Koleksi Cibinong Science Center Berdasarkan Marka RAPD dan ISSR Yuyu Suryasari Poerba; Fajarudin Ahmad
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2584

Abstract

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value which also has good dietary source. Here, the genetic variation of 20 diploid (AA) banana cultivars from Cibinong Science Center collection were analyzed. Random amplified polymorphic DNAs (RAPDs) and Inter Simple Sequence Repeats fingerprinting of these banana cultivars were carried out by four primers of RPDSs and two primers of inter simple sequence repeats (ISSRs) led to DNA amplification. The amplification products of RPADs and ISSRs were polymorphic, 97.83% and 95%, respectively. Size of the bands was varied from 350bp to 2.0 kbp. The range of genetic distance was from 0.06 to 0.07. The molecular data showed that these banana varieties were diverse collection.
GENETIC VARIATION OF WILD Musa acuminata COLLA FROM INDONESIA Yuyu Suryasari Poerba; Diyah Martanti; Fajarudin Ahmad
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 2 (2019)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (965.188 KB) | DOI: 10.11598/btb.2019.26.2.896

Abstract

Indonesia is the center of origin and diversity of Musa acuminata Colla, one of the progenitors of cultivated bananas today. However, the genetic variation of wild M. acuminata has not been studied extensively, thus this study aimed to assess the genetic variation of the Indonesian wild M. acuminata based on 10 RAPD (Random Amplified Polymorphic DNA) and 10 ISSR (Inter Simple Sequence Repeats) markers. The genetic properties of 17 populations of wild M. acuminata were analyzed by Popgene 1.32 based on Nei’s unbiased measures of genetic identity and genetic distance. Of the 443 DNA bands produced, 425 (95.94%) were polymorphic. Cluster analysis of the combined data of RAPD and ISSR produced a dendrogram which separated the population of M. acuminata (A genome) from M. balbisiana (B genome), but not from M. schizocarpa (S genome).  Nei’s genetic distance of the 17 populations of wild M. acuminata ranged from 0.3676 to 0.1634.  The highest genetic distance was observed between M. acuminata var rutilifes (from East Java) and M. acuminata var sumatrana (from West Sumatra). The percentages of polymorphic loci among the 17 populations of M. acuminata ranged from 9.93% to 39.73%. Nei’s gene diversity (h) ranged from 0.041 to 0.1418. M. acuminata var malaccensis population was the most diverse among the researched 17 M. acuminata. The high level of genetic diversity of the wild M. acuminata from Indonesia emphasizes the need for conservation and preservation of the natural population and its use in the banana breeding program.
ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR Yuyu Suryasari Poerba; Fajarudin Ahmad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.540

Abstract

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.
PENGARUH ORYZALIN TERHADAP TINGKAT PLOIDI TANAMAN GARUT (Maranta arundinacea L.) L. Agus Sukamto; Albertus H. Wawo; Fajarudin Ahmad
Buletin Penelitian Tanaman Rempah dan Obat Vol 21, No 2 (2010): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v21n2.2010.%p

Abstract

The Effect of Oryzalin on Ploidy Level of Arrowroot Plant (Maranta arundinacea L.)Arrowroot is propagated vegetative so its genetic variation is very narrow. The narrow genetic variation could be broadened through ploidy manipulation. Oryzalin could induce ploidy level of chromosomes and broaden plant genetic variation. Increase in chromosome number usually corresponds with increase stomata, corm, and starch contents. The aims of this research were to broaden germplasms of arrowroot plant by doubling its chromosomes and seeking for individual plants that potentially poly-ploidy to yield increase. This experiment had been done in experimental garden of Cibinong Science Center, February-December 2009. A five-node rhizome was soaked in Oryzalin solution of 0; 10; 20; 30; 40; 50; and 60 µM for 6 days and washed with water. The rhizome was then grown in a polybag containing soil and compost (1:1) in field with 30% shaded net. Oryzalin treatment at high concentration on arrowroot rhizome inhibited plant growth but it increased plant growth at low concentration (10 µM). Several arrowroot plants resulted from Oryzalin treatments were potential polyploid plants. Their stomata were bigger/longer, greener, more rounded, thicker, and more undulated leaves than the control. 
Co-Authors Albertus H. Wawo Diyah Martanti L. Agus Sukamto Yuyu Suryasari Poerba Yuyu Suryasari Poerba Yuyu Suryasari Poerba