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All Journal Buletin Penelitian Tanaman Rempah dan Obat BERITA BIOLOGI Buletin Penelitian Tanaman Rempah dan Obat
L. Agus Sukamto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management

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PENGARUH ORYZALIN TERHADAP TINGKAT PLOIDI TANAMAN GARUT (Maranta arundinacea L.) L. Agus Sukamto; Albertus H. Wawo; Fajarudin Ahmad
Buletin Penelitian Tanaman Rempah dan Obat Vol 21, No 2 (2010): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v21n2.2010.%p

Abstract

The Effect of Oryzalin on Ploidy Level of Arrowroot Plant (Maranta arundinacea L.)Arrowroot is propagated vegetative so its genetic variation is very narrow. The narrow genetic variation could be broadened through ploidy manipulation. Oryzalin could induce ploidy level of chromosomes and broaden plant genetic variation. Increase in chromosome number usually corresponds with increase stomata, corm, and starch contents. The aims of this research were to broaden germplasms of arrowroot plant by doubling its chromosomes and seeking for individual plants that potentially poly-ploidy to yield increase. This experiment had been done in experimental garden of Cibinong Science Center, February-December 2009. A five-node rhizome was soaked in Oryzalin solution of 0; 10; 20; 30; 40; 50; and 60 µM for 6 days and washed with water. The rhizome was then grown in a polybag containing soil and compost (1:1) in field with 30% shaded net. Oryzalin treatment at high concentration on arrowroot rhizome inhibited plant growth but it increased plant growth at low concentration (10 µM). Several arrowroot plants resulted from Oryzalin treatments were potential polyploid plants. Their stomata were bigger/longer, greener, more rounded, thicker, and more undulated leaves than the control. 
REGENERASI TANAMAN BELIMBING MELALUI KULTUR AKAR SECARA IN-VITRO L. Agus Sukamto
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1278

Abstract

Star fruit(Averrhoa carambola L.)is considered originally from Indonesia. In-vitro propagation of star fruit- is a very few.Propagation of star fruit through leaf,seedling and cotyledon culture have succeeded but its propagation through root culture has not been reported.This experiment was carried out to know the responses of star fruit root culture.Roots of young seedling of star fruit grown in-vitro were cut and cultured on one-halfMurashige & Skoog (MS)medium with or without addition of1 mg/16-ben^ylaminopurine (BA).The response of explants were determined by age of the seedlings which young seedlings were more response than the old ones. Somatic embryogenesis occurred on explan star fruit roots after 9 weeks incubation. Explants produced little callus on excised roots andprofused multiple shoots on MS with addition of BA, whereas explants only produced a few shoots without any producing callus on medium MS without any addition ofBA. This indicated that star fruit can be propagated from its root which is a very rare success of the other plant regeneration from root culture.
BIOLOGI PERBUNGAAN TANAMAN AVOKAD (PERSEA AMERICANA MILL.) L. AGUS SUKAMTO
BERITA BIOLOGI Vol 3, No 1 (1985)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i1.1367

Abstract

L. AGUS SUKAMTO 1985.The flowering biology of avocado (Persea americana Mill.). Berita Biologi 3 (1) 8 - H.- A study on the flowering biology of avocado available at Bogor Botanic Garden was made.The observation was conducted in respects to the opening and closing of the flowers, the versality of the pollen grains which are then related to the fruit production.The climatic condition as well as pollination agents which may affect on the vertilization in avocado are also discussed.
PENGARUH ORYZALIN TERHADAP TINGKAT PLOIDI TANAMAN GARUT (Maranta arundinacea L.) L. Agus Sukamto; Albertus H. Wawo; Fajarudin Ahmad
Buletin Penelitian Tanaman Rempah dan Obat Vol 21, No 2 (2010): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v21n2.2010.%p

Abstract

The Effect of Oryzalin on Ploidy Level of Arrowroot Plant (Maranta arundinacea L.)Arrowroot is propagated vegetative so its genetic variation is very narrow. The narrow genetic variation could be broadened through ploidy manipulation. Oryzalin could induce ploidy level of chromosomes and broaden plant genetic variation. Increase in chromosome number usually corresponds with increase stomata, corm, and starch contents. The aims of this research were to broaden germplasms of arrowroot plant by doubling its chromosomes and seeking for individual plants that potentially poly-ploidy to yield increase. This experiment had been done in experimental garden of Cibinong Science Center, February-December 2009. A five-node rhizome was soaked in Oryzalin solution of 0; 10; 20; 30; 40; 50; and 60 µM for 6 days and washed with water. The rhizome was then grown in a polybag containing soil and compost (1:1) in field with 30% shaded net. Oryzalin treatment at high concentration on arrowroot rhizome inhibited plant growth but it increased plant growth at low concentration (10 µM). Several arrowroot plants resulted from Oryzalin treatments were potential polyploid plants. Their stomata were bigger/longer, greener, more rounded, thicker, and more undulated leaves than the control. 
Co-Authors Albertus H. Wawo Fajarudin Ahmad