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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Matematika dan IPA AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal CARADDE: Jurnal Pengabdian Kepada Masyarakat Jurnal Mangifera Edu Jurnal Kefarmasian Indonesia Proceeding Biology Education Conference Berkala Penelitian Hayati Kaunia: Integration and Interconnection of Islam and Science Journal
Laksmindra Fitria
Universitas Gadjah Mada
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Profil Hematologi Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Jantan dan Betina Umur 4, 6, dan 8 Minggu Fitria, Laksmindra; Sarto, Mulyati
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Animal model is very important in biomedical research. Prior to apply in human or other primates, experiments using lower animals model such as rodents must be conducted (preclicical studies). Wistar rats (Rattus norveg
Ameliorative Effect of Infused Watercress on Rat Galactopoiesis following Maternal Separation Hartantyo, Rahadian Yudo; Erlitaningsih, Ucik; Gerald, Jeremia; Sari, Indah Permata; Fitria, Laksmindra; Mulyati, Mulyati
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.566 KB)

Abstract

Galactopoiesis is the maintenance of milk production. The process that regulated primarily by prolactin hormone. The research was conducted to evaluate galactopoiesis activity by measuring prolactin level and milk production continuity of lactating rats after oral administration of infused watercress. Twenty lactating rats were randomly divided into five groups (two control groups and three variations of infused watercress dose groups). Serum prolactin was measured using enzyme-linked immunosorbent assay. Milk yield was measured using indirect milk measurement method by Sampson and Jansen. Milk protein level was measured using Kjedahl method. The treatment of infused watercress (10 g/kg body weight), started from postnatal day 3 until 12, indicated ameliorative effect on rat galactopoiesis by increasing milk yield and prolactin levels, but not milk total protein.
Uji Toksisitas Oral Akut Single Dose Filtrat Buah Luwingan (Ficus hispida L.f.) pada Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Laksmindra Fitria
Mangifera Edu Vol 4 No 1 (2019): Mangifera Edu
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.364 KB) | DOI: 10.31943/mangiferaedu.v4i1.39

Abstract

Genus Ficus is important plant for health benefits. Hairy fig (F. hispida) trees grow abundantly in tropical regions and fruiting throughout the year. In West Asian countries, the fruit is commonly used as food and medicinal materials. However, in Indonesia, the fruit has not been utilized. First step in exploring the potential of natural resources for consumption is toxicity test to provide information about safety and adverse effects. This research was aimed to study acute oral toxicity of young and ripe hairy fig fruits using Wistar rats as model. Procedure followed OECD Guideline Test No.420 with modification. Pure filtrate (100 %) of young or ripe fruits were administered orally at volume 1 mL/individual on day-0 in fasting animals. Control received distilled water in the same way. Parameters observed including mortality, sublethal effects, behavior/activities, body weight, complete blood count, as well as evaluation of liver, heart, and renal functions. Results showed that values for all variables fluctuated during the experiment but eventually back into normal range. However, the number of lymphocytes elevated until the end of experiment (day-14) thus increased the total leukocytes count. Accordingly, we are preparing to conduct further toxicity tests to investigate this finding. Key words : acute oral toxicity, Ficus hispida, fruit filtrate, hairy fig, single dose toxicity
Profil Hematologi Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Jantan dan Betina Umur 4, 6, dan 8 Minggu Laksmindra Fitria; Mulyati Sarto
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 2 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i2.473

Abstract

Animal model is very important in biomedical research. Prior to apply in human or other primates, experiments using lower animals model such as rodents must be conducted (preclicical studies). Wistar rats (Rattus norveg
Potensi Propolis Sebagai Imunomodulator Pada Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar yang diinduksi Penisilin-G Laksmindra Fitria; Onny Nurrahman Marwayana
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 2 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i2.937

Abstract

Propolis is a honeybee product collected from plants sap. This resinous substance is used to build and maintain the nest. Propolis has long been declared as a safe material for health maintaining. The purpose of this research was to study the potential use of Propolis as an immunomodulator in Penicillin-G-induced Wistar rats. A total of 30 male rats aged 5 months with 200-240 grams body weight were divided into 6 groups: Control Placebo (im injection of aquabidest+oral administration of NaCl 0.9%); Control Penicillin (im injection of 21.6 mg Penicillin+oral administration of NaCl 0.9%); Control Propolis (im injection of aquabidest+oral administration of 27.0 mg Propolis); Treatment 1 (im injection of 21.6 mg Penicillin+oral administration of 13.5 mg Propolis); Treatment 2 (im injection of 21.6 mg Penicillin+oral administration of 27.0 mg Propolis); and Treatment 3 (im injection of 21.6 mg Penicillin+oral administration of 40.5 mg Propolis). Leukocytes profile (total and differential counts), body temperature, and urine acidity (pH) before and after treatment were determined as pretest and posttest data. Data were analyzed statistically using ANOVA followed by DMRT (p <0.05). Results showed that administration of Propolis significantly increased neutrophils count above the normal range (more than 4.41x103/L), whereas significantly decreased lymphocytes count below the normal range (less than 6.75x103/L). This causes the value of N/L ratio increased significantly. Administration of Penicillin and the combination with Propolis significantly increased body temperature (above 38C), while treatment with Propolis alone did not induce fever or inflammation (normal temperature 37-38C). Administration of Propolis reduced urine acidity due to the impact of the administration of Penicillin (pH 5.5-7.0). It can be concluded that oral administration of Propolis in Penicillin G-induced male Wistar rats exhibited immunosuppressive effect on lymphocytes. It was suggested as the role of Caffeic acid phenethyl ester (CAPE), the main bioactive compound in Propolis which also acts as an antiinflammatory agent. Further research is still needed to support this finding.Keywords: CAPE, immune system, immunomodulators, Penicillin, Propolis
Voluntary Diving Exercise Improves Hippocampus-dependent Learning in Rats Rahadian Yudo Hartantyo; Helen Eko Putro; Epa Yohana Toga Torop; Laksmindra Fitria; Mulyati Mulyati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11168

Abstract

Several physiological alterations that arise in response to hypoxemia conditions when diving are intended to protect oxygen-sensitive organs from the hypoxic condition. Hippocampus, known for its central function in memory formation, is sensitive to hypoxic conditions. This research aimed to study the effect of voluntary diving exercise (VDE) on the hippocampus-dependent learning performance of rats. Rats were divided into control and diving groups, with swim and voluntary diving exercises every day for 60 days, respectively. Observation of memory consolidation was carried out using Morris Water Maze (MWM) and Novel Object Recognition (NOR) tests. In the MWM test, the escape latencies in the diving group were shorter than those in the control group. Consistent results were obtained in the MWM probe trial which the time spent in the target quadrant was significantly longer in the diving group. Moreover, the diving group spent more time exploring the novel object in the NOR test. Based on the results, we can conclude that 60 days VDE significantly improves hippocampus-dependent learning capacity in trained rats.
Ulva lactuca Linnaeus Potentially Promotes Reproductive Indices and Depressive-like Behavior of Hypertriglyceridemia Male Wistar Rats (Rattus norvegicus Berkenhout, 1769) Anggoro Chandra Yulistiyanto; Mayumi Hersasanti; Rahadian Yudo Hartantyo; Laksmindra Fitria; Abdul Razaq Chasani; Mulyati Mulyati
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.57924

Abstract

Excessive consumption of fatty foods can lead to hyperlipidemia, which is often coupled with hypertriglyceridemia (HTG), a condition where blood plasma triglyceride (TG) levels elevated beyond normal levels. This condition may disturb physiological functions of the body, such as reproductive functions, and other physiological imbalances leading to chronic stress and depression. Ulva lactuca is a potential natural treatment for HTG, as it contains various nutrients to aid physiological functions. This seaweed also has high levels of Cd, which can increase depression. Therefore, research on the potential benefits of U. lactuca should be followed by an investigation of its health risks. This research aimed to examine the effects of HTG and treatment with U. lactuca on reproduction and depressive-like behavior of male Wistar rats (Rattus norvegicus Berkenhout, 1769). The data collected in this research include body weight, serum TG concentration, gonadosomatic index (GSI), serum testosterone concentration using competitive ELISA, and depressive-like behaviors assessed using the Forced Swim Test (FST) and Open Field Test (OFT). Data were analyzed using One-Way ANOVA followed by DMRT, independent- and paired-samples t-test, and Kruskal-Wallis H test with a significance value of α=0.05. Body weight, serum TG and testosterone concentration, GSI, and depressive-like behaviors were increased by the HTG condition. Ulva lactuca at the1500 mg/kg BW/day did not significantly affect body weight, testosterone concentration, and depressive-like behaviors of HTG rats. Meanwhile, this treatment significantly increased the GSI and depressive-like behaviors of healthy rats. These results suggest that Ulva lactuca treatment not only enhances gonad growth and development but also increases depressive-like behaviors.
Single-dose Acute Oral Toxicity Study of Chloroform Extract of Snake Plant (Sansevieria trifasciata Prain.) Leaf in Wistar Rats (Rattus norvegicus Berkenhout, 1769) Laksmindra Fitria; Isma Cahya Putri Gunawan; Wilda Bunga Tina Sanjaya; Maura Indria Meidianing
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 1 (2022): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69389

Abstract

Sansevieria trifasciata is one of popular ornamental plants which also believed possessing therapeutic effects due to their phytochemical constituents. Secondary metabolites of plants can be toxic to other organisms; therefore, toxicity studies must be carried out to investigate adverse effects prior to further exploration as potent candidates of medicinal plants. This research aimed to evaluate toxicity and safety of consuming chloroform extract of S. trifasciata leaf (CESTL) in acute phase using female Wistar rats as model animal. Procedure referred to OECD Guidelines for the Testing of Chemicals, Test No. 420: Acute Oral Toxicity - Fixed Dose Procedure with single-dose administration of 2000 mg/kg bw. Results demonstrated that during 14 days of the experiment, neither mortality and sublethal effects as signs of toxicity were detected. There were no significant differences during the experiment between treatment groups and control in body weight, core temperature, individual and social behavior, food and water intake, as well as hematological profile, clinical biochemistry parameters, and relative organ weight (visceral organs indices). Almost all values were maintained within normal range (baseline) with fluctuation as normal physiological dynamics appeared relatively similar in all groups. Therefore, it can be concluded that no-observed-adverse-effect-level (NOAEL) for single-dose oral administration of CESTL with the dose 2000 mg/kg bw and can be classified in the hazard of Category 5 based on Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Based on this finding, we will continue to conduct further study to assess the repeated-dose acute oral toxicity. 
UJI TOKSISITAS ORAL REPEATED DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) MENGGUNAKAN MODEL TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR Laksmindra Fitria; Rosita Dwi Putri Suranto; Indira Diah Utami; Septy Azizah Puspitasari
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3936

Abstract

Hairy fig is a tropical medium-sized tree that produces abundant fruits throughout the year. In some Asian countries, the fruits are consumed as traditional medicine and food ingredient. Meanwhile in Indonesia there has not been much use. A series of oral toxicity tests must be conducted to study the possibility of toxic effects and the safety before further exploration. Oral single dose toxicity study of young and ripe hairy fig fruit filtrate has been carried out. Results demonstrated no observed adverse effect level (NOAEL) at a concentration of 100%. This study was aimed to continue the oral toxicity test with repeated dose following standard toxicity procedure by OECD Test Guideline No. 407 with some modifications. Parameters observed were mortality, sublethal effects consisted of physical conditions and behavior, body weight, core temperature, complete blood count, as well as liver, heart, and renal functions by measuring ALT, AST, and creatinine, respectively. Sampling points on day 0, 7, 14, 21, and 28. Results showed no observed adverse effect level (NOAEL) in both young and ripe fruits filtrate at a concentration of 100% however with a tendency to cause anemia and associate with renal dysfunction. Therefore, it is necessary to perform similar method of toxicity test but with lower concentration, also continue with further toxicity tests (subchronic and chronic periods).  
UJI TOKSISITAS ORAL AKUT FILTRAT PSEUDOBULB ANGGREK MERPATI (DENDROBIUM CRUMENATUM SWARTZ.) PADA TIKUS PUTIH (RATTUS NORVEGICUS BERKENHOUT, 1769) GALUR WISTAR Laksmindra Fitria; Annisa Ridhowati; Intan Woro Prawesti
Kaunia: Integration and Interconnection Islam and Science Vol. 11 No. 2 (2015)
Publisher : Fakultas Sains dan Teknologi UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/kaunia.969

Abstract

Orchid species Dendrobium nobile Lindl. is commonly consumed by Chinese people as a natural drug that can cure various diseases. Pseudobulb is a part of the plant used for this purpose. Dendrobium crumenatum Swatrz. or Pigeon Orchid is epiphytic wild orchid, which widely found in Indonesia. As belong to the same genus, D. crumenatum could have similar potency to D. nobile. This research was carried out to study acute oral toxicity of Pigeon Orchid pseudobulb filtrate in Wistar Rats (Rattusnorvegicus Berkenhout, 1769) as initial assay prior to explore its potential as medicinal plant. Procedure followed OECD Test Guideline 425, which refers to animal welfare. Parameters observed including mortality, sublethal conditions, daily behavior, appetite, body weight, hematology test, and blood chemistry test. Results showed that no mortality was found as well as sublethal conditions that affected on health. Behavior investigation demonstrated that all individual exhibited normal activity. Furthermore, appetite increased, indicated by daily intake, which resulted in increasing body weight. Hematology test determined that no negative effects on the profile of erythrocytes, leukocytes, and platelets. Blood chemistry test stated that levels of glucose, total cholesterol, HDL, and LDL were within normal range. Levels of ALT and creatinine were also normal, indicating that liver and kidney functions were not impaired. In conclusion, Pigeon Orchid pseudobulb filtrate can be categorized as practically nontoxic. Based on this finding, exploration on Pigeon Orchid as a natural drug material can be conducted.
Co-Authors Abdul Razaq Chasani Aditya K. Karim Aditya Krishar Karim Albert Lonardo Andreas S. Budi, Andreas S. Anggoro Chandra Yulistiyanto Annisa Ridhowati Annisa Ridhowati, Annisa Chalivya Aska Rarafifi Cut M. Tiraya, Cut M. Dian Kristiawati Eko Agus Suyono Eko Agus Suyono Enggal Prayogo Epa Yohana Toga Torop Erlitaningsih, Ucik Fajar Lukitowati Gerald, Jeremia Hartantyo, Rahadian Yudo Helen Eko Putro Indah Permata Sari Indah Riwantrisna Dewi Indira Diah Utami Intan Woro Prawesti Intan Woro Prawesti, Intan Woro Isma Cahya Putri Gunawan Kholidatus Silmi Kindi Adam Lia Lavi Illiy Maura Indria Meidianing Mayumi Hersasanti Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Sarto Mulyati Sarto Mulyati Sarto Mulyati Sarto Onny Nurrahman Marwayana Paul J. Kawatu Putri Dian Islami Rahadian Yudo Rahadian Yudo Hartantyo Rahadian Yudo Hartantyo Rahadian Yudo Hartantyo Rosita Dwi Putri Suranto Rury Eprilurahman Septy Azizah Puspitasari Slamet Widiyanto Slamet Widiyanto Tantri Ajeng Salma Salsabila Tika Candra Dewi Wilda Bunga Tina Sanjaya Windah Dwi Nuraini Mappalotteng