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All Journal Pharmaciana: Jurnal Kefarmasian JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Any Guntarti
Faculty of Pharmacy, Universitas Ahmad Dahlan
Medicine & Pharmacology Public Health

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Effect of regional variation on the total flavonoid level of ethanol extract of mangosteen (Garcinia mangostana) peels Any Guntarti; Juniati Annisa; Mulki Mughniy; Fitria Rizqi
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 8, No 2, (2017)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol8.Iss2.art9

Abstract

Background : Currently, traditional medicines have been widely used by the public. One of them is mangosteen peel. Extract of mangosteen peel contains alkaloids, glycosides, steroids, flavonoids, polyphenols, and tannins. In order to ensure the quality of the extract so that its chemical content can be guaranteed, it is necessary to standardize the quality of the extract that consists of specific parameters. Objective : This study aims to determine the specific parameters using Thin-Layer Chromatography (TLC) and total flavonoid level of the ethanol extract of mangosteen peel from Kalimantan, Java, and Sumatra. Methods : This research was an explorative study. Extraction technique used was maceration with 70% ethanol solvent. The tested specific parameters, namely the identity of the extract with TLC and total flavonoid level, were determined afterward using visible spectrophotometry with the AlCl3 reagent. Results: The result of the qualitative test with TLC showed that the extract of mangosteen peel contains flavonoids, terpenoids, and anthraquinones. Total flavonoid level of ethanol extract of mangonsteen peel from Kalimantan, Java, and Sumatra were (0.301 ± 0.009); (0.398 ± 0.015); (0,747 ± 0,010) mg QE/g extract, respectively. Conclusion : Extract of mangosteen peel contains flavonoids, terpenoids, and anthraquinones. Total flavonoid level of ethanol extract of mangonsteen peel from Kalimantan, Java, and Sumatra were (0.301 ± 0.009); (0.398 ± 0.015); (0,747 ± 0,010) mg QE/g of extract, respectively.
Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication Nina Salamah; Any Guntarti; Laela Hayu Nurani
Pharmaciana Vol 13, No 1 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.471 KB) | DOI: 10.12928/pharmaciana.v13i1.25694

Abstract

Halal medicine is an interesting topic to always discuss because it is a priority choice for Muslim consumers, one of which is halal capsules. Currently, molecular biology techniques such as real-time polymerase chain reactions are rapidly developing, including for the analysis of non-halal components based on DNA sequences. This study aimed to validate the quantitative PCR method for identifying DNA in gelatin-based products and to apply the confirmation method designed for capsule samples on the market circulating in Yogyakarta to prove the halalness of these samples. Validation of the porcine DNA detection analysis method on standard extraction of porcine gelatin using primer pairs obtained in previous studies. Validated methods are used for testing market capsule shells. The qPCR method using D-loop primers is specifically capable of amplifying porcine gelatin DNA up to a concentration of 0.5 pg/µL, with a CV value in the amplification response of porcine gelatin DNA isolates (1000 pg/µL) of 0.85% which meets the test criteria using the PCR. Three samples of commercial soft capsules tested gave a positive amplification response, meaning that the samples tested contained porcine DNA, and one negative sample, which probably had non-porcine gelatin. The application of this method is also very useful for ensuring the authenticity of the capsule shell, especially from cross-contamination and counterfeiting. 
Co-Authors Fitria Rizqi Juniati Annisa Laela Hayu Nurani Mulki Mughniy Nina Salamah